Andrew John Email & Phone Number

Kharghar, Panvel, Maharashtra, India

Trombay, Maharashtra, India

Project Title: Validation and expression analysis of selected curcumin biosynthesis-associated genes from Curcuma Longa L.Overview:In this project, we aimed to analyze genetic variations in turmeric cell cultures by leveraging advanced sequencing and molecular biology techniques.Key Responsibilities and Achievements:Primer Design: Developed primers based on gene data assembled from raw gene reads obtained through Illumina sequencing, ensuring precise targeting of specific genetic sequences.Cell Culture Propagation: Propagated turmeric cell cultures using plant tissue culture techniques in specialized media, maintaining optimal conditions for growth and development.RNA Isolation: Isolated RNA using a unique protocol, achieving higher purity RNA samples which facilitated accurate downstream analysis.cDNA Conversion and qPCR Analysis: Converted RNA to cDNA and conducted quantitative PCR (qPCR) to analyze genetic variations, providing valuable insights into the genetic diversity within the turmeric cell cultures.Skills Utilized:1.Molecular biology techniques2.Plant tissue culture3.RNA isolation and purification4.Gradient and Quantitative PCR (qPCR) 5.Data analysis and interpretationThis project enhanced my skills in molecular genetics and plant biotechnology, while also contributing to our understanding of genetic variation in turmeric, potentially benefiting agricultural and medicinal applications.

Navi Mumbai, Maharashtra, India

Core Committee Member | Students Club for Bureau of Indian StandardsAmity University, MaharashtraAs a core committee member of the Students Club for the Bureau of Indian Standards (BIS) at Amity University, Maharashtra, I played a pivotal role in fostering a deep understanding of leadership and teamwork among our members. My involvement allowed me to appreciate the crucial role that standards play, not only in our professional lives but also in our daily routines.A highlight of my tenure was coordinating an industrial visit to Raptakos Brett & Co. Ltd., a renowned pharmaceutical company. This visit provided an invaluable opportunity for club members to witness firsthand how standards are implemented in the pharmaceutical industry, ensuring quality, safety, and efficiency in production processes.This role not only deepened my understanding of the importance of standards but also equipped me with the skills necessary for a successful career in any field.

Navi Mumbai, Maharashtra, India

Project Title: Starch Content Analysis in Rice Varieties Using Raman Spectroscopy.Key Responsibilities:Collected and prepared rice samples from different varieties for analysis.Utilized Raman spectroscopy to acquire spectral data of rice samples.Processed and analyzed the spectral data using advanced statistical methods including Principal Component Analysis (PCA) and Partial Correlation Linear Discriminant Analysis (PC-LDA).Skills Learned:1.Raman Spectroscopy2.Data Interpolation and Preprocessing3.Statistical Analysis (PCA, PC-LDA)4. Software Proficiency (Pruthak, LabSpec, Unscrambler X)

Navi Mumbai, Maharashtra, India

Millennium Fellowship, United Nations Academic Impact and MCN*Leadership Program Participant*- Selected as one of 31,000+ applicants for the prestigious Millennium Fellowship.- Engaged in a comprehensive leadership program focused on enhancing partnership-building and community impact skills.- Successfully completed the program and earned a certificate from the United Nations Academic Impact and MCN in recognition of my achievements.

Mumbai, Maharashtra, India

Project Title: Green Synthesis and Characterization of Gold and Silver NanoparticlesDescription:Conducted a project on the green synthesis of gold and silver nanoparticles using plant extract, focusing on their characterization and antioxidant properties.Responsibilities:1. Prepared plant extract for nanoparticle synthesis.2.Synthesized gold and silver nanoparticles using eco-friendly methods.3.Characterized nanoparticles using UV-Visible Spectroscopy and Nanoparticle Tracking Analysis (NTA).4,Evaluated antioxidant properties of synthesized nanoparticles.Achievements:Demonstrated successful green synthesis of gold and silver nanoparticles.Achieved high antioxidant activity with synthesized nanoparticles​​.

Navi Mumbai, Maharashtra, India

#Conducted in-depth research on Tuberculosis pathways, gaining a comprehensive understanding of its biochemical networks and their implications in disease progression.#Utilized "Cell Designer" software to generate structured and visually clear diagrams, effectively mapping out complex biochemical pathways.#Developed strong analytical and bioinformatics skills, enhancing the ability to interpret and present complex biological data.

Navi Mumbai, Maharashtra, India

During the course of my project under the guidance of my sir i was able learn about Chloroplast genome annotation of higher organisms.

Bachelor Of Technology - Btech, Biotechnology

Minor, Industrial Safety And Resource Management

Activities and Societies: For my coursework in ISRM, I designed a project to understand troubleshooting in modern industries. We focused.

Education record

Higher Secondary Education 12th

Education record

Secondary Education 10th STD