Andy Y. Email & Phone Number

San Carlos, California, United States

Redwood City, California, United States

Menlo Park, California, United States

Menlo Park, California, United States

South San Francisco, California

- Validated automation scripts for both Tecan Evo and Hamilton Nimbus Liquid Handling Workstations used for NGS Libraries.- Optimized the biochemistry process in the NGS library construction methods. - Developed and executed appropriate experiments to validate automation scripts for the Hamilton Star at various steps in the NGS biochemistry process construction.- Prepared & QC genomic libraries’ constructs from customer DNA through the processes of PCR, digestion & ligation assays, beads purification, quantitation, and gel analysis.- Executed quality control to assessed customer gDNA prior to sample preparation- Performed SNP genotyping on customer gDNA to confirm sample identities and gender mismatches

- Generated the infrastructure for the new QC Department by locating lab spaces, purchased equipment, wrote QC documentation, trained QC personnel, established the internal QC / QA product retain policy, set-up the incoming raw and final product inspections guidelines and product specifications. - Performed real-time and accelerated stability studies to determine expiration date and tolerances of reagents used in NGS library preparation automation platform .- Optimized and streamlined the current production process workflow to reduce COGs. - Conducted qPCR screening contamination assays to detect allelic ladder or DNA

- Assisted in the reagent stability studies that are use in generating genomic libraries andsequencing.- Performed vendor qualifications on reagents to find an alternate source supplier and to reduce costs. - Technical writer for new reagent process’s instructions and QC methods prior to manufacturing implementation; revised current documentation for process accuracy.- Performed troubleshooting on reagents in the genomic libraries processes that arises during production.- Sustained, formulated, or QC reagents to meet in-house inventory demands to support genomic libraries production utilizing Epicor (ERP System) and LIMs (Laboratory Information Management System).- Assisted accounting and finance departments in monthly physical inventory. - Trained newly hired employees in reagents formulation, functional QC assay, and data analysis.

- Worked with Quality Assurance & Control, Planning, Purchasing, and Material & Logistic and other departments to meet inventory demands for the Plan of Records (PORs) utilizing JD Edwards (ERP System) and Adobe Workflow.- Supervised a team of 5 research assistants and 1 technician for making genotyping and expression reagents.- Performed real time, accelerated, and freeze / thaw reagent stability studies to determine expiration dating.- Planned department goals, scheduled weekly projects, and reviewed reagent data for department. - Contributed to the launch of different Affymetrix's product lines.

- Formulated and performed functional testing on expression and genotyping reagents per batch records, test methods, and DOPs to meet inventory demands each quarter.- Experienced in generating Nonconformance Reports (NCMR) and deviations. - Experienced in various expression and SNPs Genotyping Assays involving but not limited to restriction digests, ligations, PCR, & RT-PCR, various purification methodologies, fragmentation, labeling, and micorarray hybridization. - Performed HPLC analytical tests & data analysis on materials used in making Affymetrix Reagents.

- Performed high-throughput universal assay to genotype mice using quantitative real-time PCR (Taqman) in a 384-well format and analyzed data.- Assumed management responsibilities in supervisor’s absence to attend meetings, review genotyping data, and report issues.- Identified unknown mouse genomic DNA sequences using a method “Genome Walker” involving BLAST searches, genomic DNA library constructions, PCR, and agarose gels.

- Assembled and analyzed mouse DNA sequences using Sequencher software to designed primers to facilitate the identification of homologous recombination events in mice knockouts used in PCR based screening assays - Performed high throughput genotyping on mouse DNA.- Imaged specific mice tissue samples for company’s DeltaBaseTM product.

- Screened for immunoglobulin subclasses (Ig) in mouse and rat utilizing immunoassay. - Generated, updated, and reviewed Department and Standard Operating Procedures to meet ISO standards. - Performed functional QC on products prior to release to Production for labeling and packaging.- Inspected final products, labels, and packaging prior to release into inventory. - Practiced Good Manufacture Procedures (GMPs) and Standard Operating Procedures (SOPs).- Tested ImmunoHistology Kits used in tissue staining.