Development of novel chimeric antigen receptors (CAR) for their use in several immune cells (including T and natural killer (NK) cells). Characterization and optimization of their use in several malignancies in vitro and in vivo.

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Development of a platform for fast recovery, optimization and screening of T cell receptors (TCRs) for cancer immunotherapy. Technologies used: molecular cloning, plasmid library generation, cell culture, transfection, retroviral transduction, antibody and tetramer staining, flow cytometry, cell sorting (single cell or bulk), high-throughput screening.

Gene network elucidation using an optogenetic CRISPR/Cas9 system. Design, simulation and implementation of an optogenetic Cas9 system for genetic network elucidation. Different gene networks were simulated in Matlab using different perturbations for further implementation in mammalian cells.

Identification of pluripotency maintenance genes in hematopoietic stem cells from mice. Main tasks consisted of analyzing the image data and cell tracking, as well as molecular cloning and stem cell extraction from mice.

A splicing control system to aid gene therapy against bare lymphocyte syndrome (BLSI). Development of a splicing control system that aims to compact gene size to aid gene therapy for BLSI. Involved in cloning, mammalian cell culture, flow cytometry and flow cytometry data analysis.Doshi J, Willis K, Madurga A, Stelzer C, Benenson Y. Multiple Alternative Promoters and Alternative Splicing Enable Universal Transcription-Based Logic Computation in Mammalian Cells. Cell Rep. 2020 Dec 1;33(9):108437. doi: 10.1016/j.celrep.2020.108437. PMID: 33264624.

Expression and study of cyclooxygenase-2 (COX-2) in mammalian cells. Development of a cell line overexpressing COX-2. Involved cloning, expression, detection and purification of the protein in mammalian cells.