Amanda Nieman, Phd Email & Phone Number

-Member of the Tailored R&D Solutions (TRS) team, which focuses on customizing Promega's technology to specific customer needs.-Develop protein-protein interaction assays using NanoBRET™ and NanoBiT® technologies.-Design, build, and characterize CRISPR edited cell lines utilizing NanoLuc®, HiBiT, and HaloTag® technologies.-Design and perform NanoBRET™ target engagement assays. -Adapt aforementioned assays to high-throughput screening using a High Resolution Biosciences automation system.-Frequently employed techniques include: CRISPR/Cas9 genome editing, wide range of mammalian cell culture, PCR, dPCR, high-throughput screening, FACS, cell cloning, Gibson Assembly, mini prep, bacterial transformation, analysis of Sanger sequencing results, acoustic dispensing-Instrumentation used includes but is not limited to BioRad dPCR using QX200 and QX One, BD FACSMelody Cell Sorter, BioTek MultiFlo FX, Beckman Coulter Echo, Agilent Bravo, Liconic automated incubators-Software used includes but is not limited to JMP, Freezerworks, Benchling, SeqMan Pro, ImageJ, FlowJo, SeqBuilder Pro, GraphPad, SAP, MS Office, MS Teams

Madison, Wisconsin Area

Chemistry & Biochemistry

Study under the guidance of Dr. Alexander (Leggy) ArnoldBackground, Planning, and Preparation• Highly adaptable and quick to understand new areas of science through self-study of primary literature, internal documents, and other internet sources• Motivated to discuss novel findings with peers to better my understanding and promote interdisciplinary work • Maximize efficiency with good time management skills such as punctuality, multi-tasking and scheduling• Create and preserve a safe work environment though laboratory preparations and inspections as a biosafety officerLaboratory Skills, Communication, and Ethics• Maintain multiple mammalian cell lines and primary murine cell cultures• Perform in vitro assays including automated patch clamp, RT-qPCR, Western Blotting, ELISA, and high through-put fluorescence and luminescence-based assays• Execute in vivo studies using behavioral assays and disease models• Generate highly reproducible results, visualize data using analysis software such as MS Excel and GraphPad, and ethically report findings to supervisor• Collaborate with peers in other science fields to ensure laboratory safety, communicate results of shared interest, and perform interdisciplinary experiments• Work in shared workspaces and care for common equipment Mentoring and Soft Skills• Mentored new scientists in the laboratory • Coordinated learning outcomes with course instructors• Generated lesson plans and quizzes

Mequon, Wi

-Assisted the Quality Assurance team through product testing-Wrote standardized work procedures for a variety of production tasks-Created Microsoft Access database for labor tracking

Milwaukee, Wi

-Mentor: Dr. Edward Blumenthal-Investigated the possible interactions of genes with Drop Dead in Drosophila melanogaster-Techniques Utilized: PCR, RT-qPCR, gel electrophoresis

Doctor Of Philosophy (Ph.D.), Biochemistry

Bachelor Of Science (B.S.), Biomolecular Engineering

Activities and Societies: Society of Biological EngineersChemistry Minor Mathematics Minor

High School Diploma

4 years High Honors, Badger Girls State, Arion Music Award