Ankit Gupta, Ph.D. Email & Phone Number

Redwood City, California, US

Redwood City, California, US

Critical Reagent ManagementSPRX Protein and Cell process development Generation and Characterization of critical reagents/monoclonal antibodies/polyclonal antibodiesFormulation development SPRX Potency Assay developmentGLP Stability Studies to support clinical programsProcess Characterization execution Involved in FMEA and DOE design

Redwood City, California, US

Biologics Downstream Process DevelopmentDevelopment/Tranfer of Potency Assays using AlphaLISA for stability testingGeneration and characterization of critical reagents for various clinical and non clinical assaysSupport R&D for lead identification and characterization using BiacoreCrystallographic studies of Target D domain complex esLead work with.

Redwood City, California, US

CAR-T: Working in the area of lead discovery, optimization and development. Supporting multiple discovery/development programs through characterization of lead target interaction by Biacore and purification of lead candidates for animal studies. Also, development of dual potency assays using Biacorewww.arcellx.com

New York, NY, US

- Directly involved in setting up the new lab at NYU Cancer Center- Instrumental in the purchase of the Pioneer system by comparison of various SPR-based technologies using the GFP and GFP-binding monobody pair - Optimized and established working protocols for ligand immobilization and regeneration with the Pioneer SPR system - Established new protocols.

Chicago, IL, US

• Designed, executed, analyzed and interpreted data for multiple projects on controlling epigenetic regulation using designer binding proteins based on the fibronectin scaffold called monobodies.
• Utilized phage display and yeast display libraries to select high affinity and specificity monobodies against therapeutically relevant epigenetic methyltransferases and reader domains.
• Completed a project in collaboration with a research group at Stanford University to understand the molecular mechanism of PRDM14 in germ cell development contributing by developing PRDM14-specific monobodies, in vitro.
• Collaborated with research groups at Univ. of Chicago, Univ. of Michigan and Ludwig Boltzmann Institute for Cancer research to investigate the phenotypic consequences of expressing WDR5-specific monobodies in various.
• Supervised and trained two research technicians that led to the development of specific monobodies to BET-family of bromodomains.
• Supervised an undergraduate student on a Ras/monobody crystallography project towards a work that was published in Nature Chemical biology

Kharagpur, West Bengal, IN

• The Students’ Alumni Cell IIT Kharagpur, is promoting its initiative “The Student Alumni Mentorship Programme” which offers the students the unique opportunity of interacting on a personal level with the well-placed.
• Mentored several undergraduate students from the Department of Biotechnology with career planning, graduate school applications and job opportunities in the pharma/biotech sector.

Rochester, NY, US

• Thesis: Role of a novel domain in SF1/U2AF complex formation and 3' splice site recognition
• Demonstrated using NMR and ITC that a highly conserved novel domain in SF1 is involved in SF1/U2AF65 interactions key to the first step of pre-mRNA splicing
• Established by ITC that the central domain in SF1 is essential for the cooperative RNA recognition by SF1/U2AF65 complex
• Utilized X-ray crystallography and Small angle X-ray scattering to characterize the role of SF1 central domain in conformational rearrangements in early stages of splicing
• Developed fluorescence based EMSA assays and methods for labeling of protein expressed in E.coli.
• Trained undergraduate and junior graduate students to use several biophysics instrumentation such as ITC, Fluorimeter and overseeing their protein purification strategies using AKTA FPLC systems.

Kharagpur, West Bengal, IN

• Computational identification of surface protein in Entamoeba histolytica as potential vaccine candidate and drug target
• Predicted new surface proteins in Entamoeba histolytica based on the presence of signal peptide, transmembrane domain, subcellular localization and glycosylation patterns.
• Developed a comprehensive Entamoeba histolytica proteome MySQL database containing information about various signals

Kharagpur, West Bengal, IN

• Computational identification of encystation specific gene in Entamoeba histolytica
• Identified consensus sequences in the promoter region of encystation specific genes
• Examined proteins predicted in the cell wall of Entamoeba histolytica for encystation specific consensus sequences in the promoter region

Trieste, IT

• Use of "Codon shuffling"​ method for the generation of denovo proteins and peptides
• Developed a comprehensive database of William P.C.Stemmer's and Frances H. Arnold's publications on directed evolution of enzymes and proteins.
• Developed databases containing secondary structure information for all combinatorial sequences of two unique heptad repeats used in the "codon shuffling" strategy
• Assisted in the design and expression of Thioredoxin template proteins used for preparing the library of fusion proteins screened for antimicrobial properties

Delhi, Delhi, IN

• Purification of phosphotransferase Spo0F and Spo0B and cloning of negative regulator phosphatase spo0E gene from Bacillus anthracis
• Expressed and purified milligram quantities of response regulator, Spo0F and phosphotransferase, Spo0E from Bacillus anthracis in E.coli BL21 cells
• Cloned the negative regulator phosphatase, Spo0E from Bacillus anthracis in pHTX, pGEX and YS5 expression plasmid vectors.

Phd, Biophysics And Structural Biology

M.S., Biophysics

B.Tech And M.Tech, Biotechnology And Biochemical Engineering