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During my studies in Biotechnology (Bachelor’s degree at Milano-Bicocca University and Master’s degree at University of Brescia) I cultivated my passion for science and in particular for Biology.I performed several traineeships learning about Molecular Biology and also approaching to veterinary world. During that period I acquired theoretical and practical knowledge about cloning techniques for recombinant antigens' expression in bacteria and higher organisms in order to realize a diagnostic kit (ELISA test).My experience and participation in different projects during my master years in different research centers (Italy and Spain) has let me meet the new growing exosome and nanotechnology fields. During my master’s thesis in Barcellona I also improved my English communication skills, team working and networking.In Italy I worked on a diagnostic ELISA test for ovarian cancer detection in Bird's R&D, where I acquired fundamental skills for my carrier, such as, project planning and problems solving. I also worked for a veterinary and pharmaceutical company, FATRO Spa, as Quality Control Operator, focusing on cell coltures, used for avian vaccines check.I won a scholarship at IZSLER for a project about influenza A virus. In particular its aim is replication activity and pathogenesis evaluation of swine influenza A viruses using reverse genetics techniques, respiratory organoids and 3D cell coltures of swine origin. Now I am a researcher at IZSLER, Unit Vesicular Viruses and Biotechnology Products and I am following several projects.I am a multitasking, easygoing and dynamic person and my aim is to help to the development and execution of ideas and projects sharing motivations with colleagues to achieve goals. I am also highly motivated to work to improve people's health and like facing new challenges.
Izsler - Istituto Zooprofilattico Sperimentale Della Lombardia E Dell'Emilia Romagna
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Scientific ResearcherIzsler - Istituto Zooprofilattico Sperimentale Della Lombardia E Dell'Emilia Romagna Mar 2022 - PresentBrescia, Lombardia, Italia -
Research FellowIzsler - Istituto Zooprofilattico Sperimentale Della Lombardia E Dell'Emilia Romagna Nov 2019 - Feb 2022Brescia, Italia"Evaluation of swine influenza viruses replication and pathogenesis using reverse genetics techniques, respiratory organoids and 3D swine culture cells" -
Quality ControlFatro Spa Jun 2018 - Nov 2019Brescia, ItaliaQuality control of avian vaccine.Determination of virus titre (TCID50 and EID50) using both fibroblast embryos cells (CEF) and embryo chicken eggs. Main tested virus are Reovirus, Marek’s disease virus (MDV) andinfectious bursal disease virus (IBDV).Other ordinary activities are ELISA tests, serological test such as hemagglutination assays (HA) and hemagglutination inhibition assays (HI), microbiological and chemical tests.Draw up vaccine’s documentation and new procedures updating
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DipendenteBird Srl Nov 2017 - Jun 2018Brescia, ItaliaI worked on a diagnostic ELISA test for ovarian cancer detection in Bird's R&D:- reagents selection and characterization;- suppliers selection in accordance with ISO procedures;- procedures importation from partner, patent holders, to Bird;- preliminary protocol application and kit implementation for analytical performances control;- raw materials and reagents stability studies;- statistical data analysis;- intra and inter assay tests and quality control procedures;- mathematical algorithm development based on the analysis of LOD and CVs;- draw up project reports;
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DocenteCasa Dello Studente Cds Jan 2016 - Nov 2017Brescia, ItaliaDocente presso la filiale del Villaggio Sereno (BS) di Casa dello Studente di Matematica, Fisica, Chimica, Biologia e Inglese principalmente.
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Master ThesisGermans Trias I Pujol Research Institute (Igtp) May 2017 - Sep 2017Badalona, Spagna"Accurate determination of the amount of exosomes secreted by human reticulocytes by integrating colloidal nanoplasmonics and light scattering techniques" In University of Brescia I learnt how to isolate extracellular vesicles using colloidal system and to characterize them with different assays such as western blot, Atomic Force Microscopy (AFM) and colorimetric nanoplasmonic assay (CONAN).The aim of this project is to develop a robust and accurate method for the determination of the basic colloidal properties, including molar concentration, size distribution and stability in common buffers, of exosomes isolated from culture media of human reticulocyte cell lines and from THP1 cells and porcine serum. I compared and inter-calibrated two techniques, Nanoparticle Tracking Analysis (NTA) and a colorimetric nanoplasmonic assay (CONAN), in order to obtain a robust quantification of exosome formulations. In particular I focused on reticulocytes-derived exosomes (rex) because they have an important role in Malaria pathogenesis. Two main themes of my thesis were the inter-calibration and the isolation and characterization of exosome derived from different sources (reticulocytes, THP1 cells and porcine serum). I used different exosome isolation protocols: sucrose gradient and size exclusion chromatography. Exosomes were characterized by bead-based assay (using an alternative FACS analysis) for exosomal markers and with BCA assay for the presence of exogenous proteins (contaminants). I inter-calibrated CONAN and NTA, which have different physical-chemical principles, and the fact that was possible is an important result. The inter-calibration curve allows to choose the method that better fits exosome preparation. I provide the first steps in quantification of rex. -
Stage Extracurriculare Post-LaureaIzsler - Istituto Zooprofilattico Sperimentale Della Lombardia E Dell'Emilia Romagna May 2015 - Oct 2015Brescia, ItaliaPost Bacherlor's degree Internship of 5 months. Cloning techniques for viral antigens' expression in bacteria and higher organisms in order to produce diagnostic kit based on monoclonal antibodies. Primers and restriction enzymes choosing, PCR, purificaion and quantification (Nanoquant) of protein insert. Plasmid digestion, dephosphorilation and quantification. Plasmid-insert ligation, dialysis, transformation in bacteria, colony PCR, pre-inoculum, MINI sequencing and quantification. -
Stage Curriculare Di 250 OreIstituto Di Medicina Molecolare Angelo Nocivelli Feb 2013 - May 2013Brescia, ItaliaBachelor's degree Internship: Molecular biology techniques for Agammaglobulinemia's study, such as DNA extraction, PCR, electrophoresis, sequencing. I worked also with DC: isolation, activation, RNA extraction and quantification, RNA reverse transcription in DNA, western blot and Real time PCR.
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What company does Anna Castelli work for?
Anna Castelli works for Izsler - Istituto Zooprofilattico Sperimentale Della Lombardia E Dell'emilia Romagna
What is Anna Castelli's role at the current company?
Anna Castelli's current role is RIcercatore sanitario.
What is Anna Castelli's email address?
Anna Castelli's email address is an****@****sler.it
What schools did Anna Castelli attend?
Anna Castelli attended Università Degli Studi Di Brescia, Università Degli Studi Di Milano-Bicocca.
What are some of Anna Castelli's interests?
Anna Castelli has interest in Da Ormai 13 Anni Gioco A Pallavolo, La Vittoria, Animal Welfare.
What skills is Anna Castelli known for?
Anna Castelli has skills like Biochimica, Biologia Molecolare, Pcr, Microbiologia, Biotecnologia, Clonazione Molecolare, Immunologia, Estrazione Di Dna, Isolamento Di Rna, Management, Western Blot, Sistema Operativo Real Time.
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