Anna Gibbard Email & Phone Number

10+ years of Upstream processing/troubleshooting experience in cell culture vial thaw thru operating bioreactors in a GMP compliant environment at 10L, 200L, 1000L and 2000L scales in both stainless and disposable formats. Extensive experience in authoring both SOP and Batch Records, as well as training new hires, consumables procurement, and.

Exton PA

5+ years of Upstream processing experience operating bioreactors in a GMP compliant environment at 10L, 200L, 1000L and 2000L in both stainless and disposable formats. GMP compliant experience in cell culture processing from vial thaw thru bioreactor inoculation, including flask steps and wave bioreactors. GMP compliant experience in Upstream process.

Upstream Operations

Responsible for supporting and executing the commissioning, startup, and routine operation of the laboratory and production equipment in a cGMP biopharmaceutical pilot plant

Lead Discovery

• Generate target-specific antibody-producing hybridomas using In Vitro Germinal Center reactions and single B cell culture
• Retrieval of antibody heavy and light chain sequences from hybridomas generated in IVGC by PCR amplification for multiple species, including human, mouse, rat, and rabbit, and transfer of DNA product to downstream.
• Analysis of multi-species biomarkers multi-color FACS
• Coordinated generation of human cDNA library of antibodies from SLE patients… Show more
• Coordinated generation of human cDNA library of antibodies from SLE patients using single cell PCR
• Dissect, isolate and culture lymphoid cells from rat

• Cell biologist for Cancer Stem Cell team within Oncology division.
• Generate and analyse siRNA knockdown data using RT-PCR and Western Blot
• Optimize gene signature of β-catenin pathway for cell-based compound screening
• Maintain a variety of cell lines for use in cell-based reporter assay experimentation
• Utilize cell-based compound screening viability assays using various robotics.

• Developed acute inflammation models for murine peritonitis and uveitis assays for the Pattern Recognition Receptor Department, in the Immuno-Inflammation Center for Excellence in Drug Discovery.
• Generated ELISA and Western Blot data for therapeutic area teams
• Coordinated RNA profiling activities in the Immuno-Inflammation division.
• Analyzed RNA integrity for acute immunity rodent models.
• Data analysis for gene expression studies and SOP documentation.
• Developed assays for… Show more

• Technology expert in the Taqman platform supporting numerous disease areas within the company including Oncology, Osteoarthritis, Immunology and Inflammation, and Supportive Care
• Highly skilled in all aspects of planning and performing experiments using Real-Time PCR Taqman gene expression analysis, including RNA isolation and quantification, quality control, cDNA conversion, and primer set.
• Expert in analysis of gene expression data by integrating the use of Spotfire… Show more
• Expert in analysis of gene expression data by integrating the use of Spotfire, Microsoft Excel, internal analysis tools and external literature to present results.
• Tests new technologies, including a novel method of analyzing activities of transcription factors within signal transduction pathways integrating expertise in cell culture, PCR amplification, RT-PCR and DNA sequencing..

Summers of 2002 and 2003 I worked in a research lab assisting with analysis of Chlamydia pneumoniae infection and Alzheimer's disease pathology in the human olfactory system.