Anna Knize Email & Phone Number

San Diego, Ca, Us

California, United States

San Diego, California, United States

Greater San Diego Area

San Diego

• Biomarker Assay for Phase 1 Clinical Trialo Responsible for development, validation, and execution of an LC/MS based substrate hydrolysis biomarker assay in human patient peripheral blood mononuclear cells • Human Whole Blood Functional Assays Leado Established and executed hWB assays for ex vivo analysis of small molecule IC50’s, time dependent activity, reversibility, and enzyme recovery o Determined risk profile of small molecules on COX pathways via eicosanoid analysis by LC/MS and inflammatory markers by ELISA in stimulated hWB and primary human cells• Metabolomics, LC/MSo Profiled lipid metabolites treated with small molecule enzyme inhibitors in CNS and peripheral tissues for multiple programso Managed and trained staff in lipid metabolite extraction techniques and analysis on Agilent triple quadrupole LC/MS system• Disease Patient sample profilingo Lead the characterization of inflammatory cytokine expression and lipid metabolites in human CSF from Alzheimer’s Disease patients o Evaluated serine hydrolase enzymes in Inflammatory Bowel Disease patient samples and treated cell lines to establish new enzyme targets

San Diego, California

3545 John Hopkins Ct. #250

• Evaluated a murine bispecific biologic inhibitor as a potential treatment for autoimmune disease. Bispecific inhibitor was tested in a sheep red blood cell challenge model and NZB/NZW lupus model with endpoint analysis by FACs and ELISA. Determined dual target blocking activities in human B and T cell assays by 3H incorporation. • Developed assays for target validation studies and compound screening of small molecules utilizing human T and B cells and murine B cells for Ig class switch analysis• Evaluated T and B Cell regulation in human tonsil, spleen, lymph node, and PBMCs• Responsible for comparing the effects of antibody candidates through cytokine, immunoglobulin, and drug serum levels by ELISA and MSD

• Led the co-culturing of stem cells and organotypic hippocampal slice cultures to study the integration of grafted cells with host tissue by way of immunofluorescent cell labeling• Facilitated the implantation, replacement, and maintenance of subcutaneous osmotic pumps in mice for a collaborative study of the efficacy of a drug treatment candidate• Responsible for evaluating electrophysiological properties of neuronally differentiated human bone marrow stem cells by whole cell patch clamp • Isolation of rat organotypic hippocampal slice culture and primary neurons to study neuroprotection and synaptic plasticity• Responsible for sectioning, staining, treatment, and analysis of fresh frozen rat brain for in situ analysis of neuroprotective treatments

• Participated in development and optimization of cell sorting and culturing techniques of human bone marrow for adult pluripotent stem cells using FACS analysis and antibody recognition techniques• Developed and conducted molecular analysis for cell characterization of pluripotent stem cells• Responsible for presentation of research data at weekly meetings to executive management