Phd Student
Inserm U1013
Université Paris V Descartes, Paris,Doctorate in Immunology, passed with distinction.Subject: IDE Knockout protects NOD mice from diabetes by attenuating the inflammatory process and delaying the autoaggressive T cell response. Throught my data we could gain a research grant from the Juvenile diabetes research foundation for 1 year over 93.000 Dollar, a research grant from the "agence-nationale-recherche (ANR)" over 250.000 Euro for 3 years, and a grant from the "Fondation pour la recherche médicale" over 30.000 Euro for one year. Cell culture Primary CD8+ culture, bone marrow derived dendritic cell culture, extraction of different cellular subsets by MACS sorting, HeLa cell culture Cell biology Organ cuts in paraffin, cryocuts, immunofluorescence staining, fluorescence microscopy, staining of cells for FACS analysis, ELISPOT analysis of activated T-cells, isolation of pancreatic islet cellsMolecular biology Cloning, cell transfection, bacterial culture, protein analysis by western blotting, extraction of DNA and RNA, quantitative und real time PCR, ELISA of mice sera and cellular supernatantsWork with miceDissection, glycosuria tests, irradiation, bone marrow transplantation Mentor: Professor Peter van Endert, M.D, Medical Faculty of University Paris-Descartes, Department of Immunology