Anna Nummy Email & Phone Number

Ann Arbor, Michigan, United States

Ann Arbor, Michigan, United States

Howell Michigan

We provide customer opportunities to local, established, businesses so they can focus on what they love doing most. We also help our clients understand how to turn a customer opportunity into a paying client. We use both long-term growth strategies (SEO) and paid advertising (Google, Facebook, Instagram) to generate immediate customer opportunities. We offer a wide variety of services including mobile friendly website design and social media marketing. We specialize in Facebook ads for many industries, including chiropractic, salon, roofers, personal trainers, home & auto insurance, dealerships, dog walkers and trainers, and more.

I design and hand make jewelry using metalsmithing techniques.

Ann Arbor Michigan

Assay Development- Responsible for developing new assays and writing Standard Operations Procedures (SOP’s) and protocols including feasibility, characterization, qualification/validation.Process Development support- Participates in several studies and developing new methods for growth of autologous stem cells for therapy for clinical trials.Technical transfer of procedures and assays to QC lab.Assists in trouble shooting and investigations related to assays performed in the QC group.Keep abreast of requirements for compliance (cGMP, cGCP, FDA Guidance for Industry Method Validation).Familiar with principles of statistical analysis and JMP software.

Ann Arbor Mi

Ann Arbor, Michigan

While in Molecular Biology, cloned various proteins for expression in E.coli and Baculovirus (BV) systems using signal sequences, affinity tags, and truncations to produce soluble active proteins. Used ABI 3130 sequencer for in-house sequencing of clones. Experienced cell culturist; insect and mammalian. Responsible for cell maintenance/passage and infection, plaque assays, and MOI studies, and cryopreservation. While in Protein Core, performed expression studies, ran SDS-PAGE, and Western Blots. AKTA FPLC protein purification's include gel filtration, and ion exchange chromatography, and affinity chromatography. Characterizing pure proteins using known techniques as well as developing assays for both in-house use and commercial sale (Malachite Green Assay, sEH Inhibitor Screen). Assay characterization included determining IC50, Km, and determining specific activity for proteins. Independent interpretation of assay data and troubleshooting was a routine part of my job.

Molecular Biology group- Independently performed cloning experiments including PCR, gene recombination, gel electrophoresis, SDS PAGE, bacterial transformation/selection, and DNA purification among others. Experience with T7 bacterial expression system and expression in Yeast. Pilot Plant: Product runs/small scale fermentation.

Lansing, Michigan Area

Worked in the Blood Derivatives Procurement lab as a Plasma Technician. Duties includedpreparing plasma for fractionation, preparing and working in a Class 3 Clean Room, sampling plasma lots for QC testing, and processing plasma recalls. Followed Standard Operating Procedures (SOP) using current Good Manufacturing Practices (cGMP).