PROJECT: Role of MAP kinase MAPK-15 in ciliogenesis in C. elegans and human cells• Identify and characterize a novel role for MAP kinase MAPK-15 in primary cilia formation• Spearhead the effort of protein labeling using CRISPR/Cas9 system in the laboratory• Perform Confocal microscopy in live animals and fixed cells• Facilitate and manage collaborations with several academic institutions in the U. S. and Ireland• Train and supervise 1 undergraduate and 2 graduate students on 5 projects• Optimize and troubleshoot protein purification for common laboratory use• Maintain a large collection of C. elegans strains

• Supervise and teach 2 group sessions 25 students each• Prepare a short lecture on topics that were not covered during the class• Organize study sessions to help students prepare for the exams• Grade quizzes and exams• Help with homework assignments

PROJECT: Regulation of cell wall synthesis in S. cerevisiae by Rho1 GTPase• Identify a new mutation in Rho1 that interferes with cell wall synthesis pathway• Genetically engineer a large collection of S. cerevisiae strains to monitor protein localization• Monitor protein phosphorylation state using Western blot analysis

• Supervise a General Biology I Laboratory Course (25 students)• Prepare an introductory lecture to go over the experiments and background• Help with preparation of lab reports• Grade lab reports and exams

• Supervise a General Chemistry I Laboratory Course (17 students)• Grade lab reports• Prepare a small introductory lecture

PROJECT: Identification of proteins involved in NETO (new end take off) in S. Pombe cells• Perform basic genetics experiments in S. Pombe cells• Image and Analyze Confocal microscopy data• Purify proteins from S. Pombe cells to be used in binding assays

PROJECT: Purification and activity determination of recombinant human alpha-galactosidase A• Grow large volumes of Pichia Pastoris for recombinant protein production• Perform spectrophotometric enzyme assays• Analyze and plot data to determine alpha-galactosidase A enzyme activity• Present my results during an Undergraduate Honors Thesis Lecture Series• Submit my results as part of my Undergraduate Honor Thesis

PROJECT: Structure and Dynamics determination of the P7 Protein from the Bacteriophage phi12• Perform simple cloning experiments - generate His-tagged protein expression plasmids• Perform early steps in protein purification - buffer preparation, sonication