Ann Devine Email & Phone Number

Philadelphia, Pa

March of Dimes grant that focuses on placental function. To study the elevation of acylcarnitines and increased prostaglandins in sPTB placentas relative to term birth placentas 1. Trophoblast isolation – Proficiency in isolation of trophoblasts from third, second and first trimester placenta 2. Developed second trimester vilous explant model 3. Migration to LabVantage inventory system from the previous data baseMarch of Dimes grant that focuses on mitochondrial mutation. To test the hypothesis that there are high levels of mtDNA mutation present in sPTB infant, mother or reproductive tissues compared to controls. Determine if there is a change in predominant sequence associated with sPTB 1. mtDNA isolation from maternal blood, cord blood and placental biopsies2. mtDNA amplification and mtDNA repair for amplicon submission for sequencing 3. Sample submission into MOD data baseMarch of Dimes grant that focuses on the vaginal microbiome, host immune response and cervical remodeling.Study the mechanisms by which changes in the cervicovaginal microbiota may promote disruption of the cervical epithelial barrier and premature cervical remodeling. Investigate how microRNAs regulate the cervical epithelial barrier and how they might be involved in premature cervical remodeling. Perform message rna profiling in both ecto and endocervical cells to identify the downstream targets altered by overexpression of miRNAs and regulation of these mrna in inflammation-induced remodeling and PTB.

Huntingdon Valley, Pa 19006

Director of Cellular Research: Dr. Mark ConnellySupervisor: Manager of CellSearch R&D: Dr.Chandra RaoDevelopment of an FDA approved reagent assay kit and methods for the detection of circulating tumor cells from blood*Create novel marker reagents and detection of rare cells using the Veridex CellSearch System*Adapt known biomarkers for analysis of CTC’s in model systems and cancer patient samples*Conduct assay development and product development to written and established Design Control Specifications*Develop raw material, in process and final release of the QC assay to assure product performance and compliance to product requirements as set down in Product Requirements Documentation*Manage all aspects of the laboratory operations, instrument QC, supply ordering, coordination of resources shared across groups and routine operator maintenance.

West Chester, Pa (Contracted By Aerotec 4/2006 Hired By Cephalon 1/2007

Vice President of Research: Dr. Jeff VaughtDirector: Dr. Bruce RuggeriSupervisor: Dr. Susan Jones-Bolin, Dr. Matt SeaveyContracted to perform in vitro, cell based assays and in vivo assays for the Bendamustine project and to advance lead compounds into drug development.*Determination of bendamustine concentration in various cell lines using the MTS assay*Performed clonogenicity cell survival studies using the dose of bendamustine determined from the MTS assays*Developed a research protocol for in vivo glucose tolerance testing *Developed a lung carcinoma model in mice for radiation studies*Developed a breast cancer metastatic mouse mode that metastasized to the lymph nodes from a cell isolate that I developed*Performed pk studies as needed*Proficient in multiple I.V. dosing*Determination of Antibody binding to various cancer cell lines by flow cytometry

Spring House, Pa

Team Leader: Dr. Dana JohnsonSupervisor: Dr Jan Sechler*Performed in vitro, cell based and in vivo assays for the c-Met project to advance lead compound into drug development Primary screen:c-Met in vitro kinase autophosphorylation assay. Secondary c-Met Cell Elisa assay*Developed assays for the assessment of in vivo activity S114 Mouse Tumor Xenograph PD assay Metastatic model for the orthotopic injection of MDA-MB- 435S into mammary fat pad*Supported additional team projects for PDGF-R and Tubulin programs: Cell proliferation Abl kinase assay PDGF-R kinase and autophosphorylation assay

Spring House, Pa

Director: Dr. Charlie BowdenSupervisor: Dr. Dave End, Dr. Bob Tuman*Responsibilities for the Farnesyltransferase project were directed to the advancement of Zarnestra for clinical developement and FDA approval Cell Proliferation Support clinical work for Zarnestra with apoposis studies using flow cytometry Immunoblotting for different ras mutations Develped a mouse model for cachexia in collaboration with Centocor

Spring House, Pa Robert Wood Johnson Research Institute

Director: Dr. Jeffery VaughtSupervisor: Dr. Barry DubinskyResponsibilities for the Anxiolytic project were a variety of in vivo assays to determmine bendodiazepine binding, behavioral pharmacology and safety of novel compounds Metrazol induced seizure Conflict test Ethanol Induce narcosis Precipitated withdrawal Cloniding induced madriasis Apomorphine induced climbing CNS test to obtain a broad spectrum classification of CNS effects of compounds based upon direct observation of drug induced responses in mice

Great Valley, Pa

Purification and processing of antibodies for medical diagnostic kits and running quality assurance tests for antibody purity

Philadelphia, Pa

Supervisor: Dr. Michael C. PhillipsSeparation and electrophoresis of lipoprotein fractions. Support work for the graduate students with electronmicroscopy and chromatofocusing.

B.S., Biology

Education record