• Develop and carry out analytical assays to measure critical quality attributes of Cellino’s products (iPSCs, iPSC-derived cells) following ICH, FDA, and EU guidelines.• Run analytical tests such as DNA/ RNA isolation, RNAseq library prep, WES library prep, qPCR, flow cytometry, endotoxin, and sterility (bacteriostasis/fungistasis)• Perform cell culture maintenance on iPSCs and CD34 cells• Sample management of donor samples and iPSCs• Maintain necessary documentation, including SOPs and records of QC assays outcomes• Write assay protocols • Perform Root Cause Analysis on contamination issues• Perform internal audits of ELNs and cell culture assays• Conduct GMP/GLP/GDP training to employees • Train new employees on cell culture and analytical QC assays• Supervise major QC projects and research associates involved in the projects• Stay up to date on the latest science and development in the QA/ QC space

-DNA and RNA purification/extration using the SenSATIVAx® method on hemp, flower and cannabis-infused products-Microbial safety testing using PathoSEEK® qPCR method-Microbiology culturing on various yeast, mold and bacteria-Data analysis from the Agilent Aria and BioRad instruments

-Performed QC tests such as qPCR,Immunocytochemistry, Flow Cytometry, sterility, and endotoxin detection for pre-clinical material-Developed and qualified qPCR assay to test for Mycoplasma-Maintained and stocked lab supplies -Validated and qualified QC assays-Performed IOP/Q of QC equipment-Authored assay protocols, reports, and forms-Grew cell banks for human dermal fibroblasts and embryonic stem cells

• Execute biochemical and bio assays for characterization of protein therapeutics – specifically with ELISA • Maintain cell lines and PBMCs • Maintain liquid handling robots such as Bio-Tek plate washer and plate reader • Run Western Blots and SDS-Page to identify and separate proteins • Order lab supplies and ensure lab is fully stocked

• Supported lentiviral vector platform• Performed analytical testing of lentiviral vectors using qPCR and p24 ELISA kit• Participated in mock inspections• Data verification and analysis of assays• Cell culture of human osteosarcoma cells

• Maintained multiple human cell lines at a time• Lysed human, mouse, and rat tissues• Performed QC on tissues by Western Blot• Cell and tissue treatments• Cell line transfections• Maintained laboratory information management systems (LIMS and JIRA)• Presented at group and company meetings

Monoclonal Development• Followed mammalian cell culture techniques.• Maintained and screen rabbit/mouse cells for multiple targets each week with a strict timeline.• Target validation via enzyme-linked immunosorbent assay (ELISA).• Cryopreservation of cells for further research.• Cloned selected cells.• Managed and maintained Rapid Plate Machines

Monoclonal DevelopmentPrepared rabbit and mouse monoclonal antibodies (mAb).Specific duties included preparing and performing ELISA, maintaining and troubleshooting ELISA equipment, making culture media for mAB development, and ordering supplies.

Provided circulation services to adults and children, including checking library materials in and out,reserved books, answered telephone inquiries and general reference questions, as well as assisted patrons in navigating the library catalog. Provided reference and reader’s advisory service, processed interlibrary loans, filed reserves and input library materials into computerized database.

Assisted the Pathologists' Assistant in gross examination and sectioning of organs and autopsies of amputated limbs. Performed hematoxylin and eosin staining procedures on human tissue and researched various stages of tumors. Presented my experience at the Undergraduate Research Symposium at Salem State University.