Anne Hett Email & Phone Number

Edinburgh, Gb

Edinburgh, Gb

Edinburgh, Scotland, United Kingdom

Edinburgh, United Kingdom

⇒Development of assays appropriate for Characterisation an Safety testing of human embyonic stem cell lines for human application, ⇒Validation of new techniques and training others, ⇒Completion of all GMP documentation with the QC department including incident reports, Change controls and Risk assessments,⇒Collaboration with the Cell Therapy development team for new assays required to support methods that will ultimately be transferred to the GMP QC team⇒to oversee and train members of staff as applicable, validation of equipment and maintaining calibration and servicing records⇒devise, plan, execute, analyse and report experiments⇒routine QC batch releasing of cellular therapies manufactured to a GMP standard

Glasgow, United Kingdom

performing CDC, ADCC, ELISAs and cell culture to GMP standardsDevelopment of new protocolsDocumentation to GxP standardsData analysis and evaluationwriting of reports for clients

Edinburgh, United Kingdom

Investigating the phenotype in human cells upon splicing inhibition using different splicing inhibitors including antisense morpholinos and drugs like Pladienolide B. I successfully established RNA-FISH and Immunofluoresence in the lab to analyse the localisation of RNA transcripts and splicing factors upon splicing inhibition, Deconvolution Microscopy (image analysis with ImageJ and softWoRx), qPCR (real-time PCR) to monitor and analyse RNA stabilisation upon splicing inhibition, various cell culture techniques including RNAi, transfection of morpholinos and plasmids, manufacturing of stable cell lines

Edinburgh, United Kingdom

Investigating the role of two uncharacterised nucleoporins during mitosis in human cells (HeLa and HEK). Live-cell and fixed cell imaging of stable cell lines expressing either C-terminal or N-terminal GFP tagged cDNAs of the nucleoporins. Immunofluoresesence of myc-tagged wild type or phosphomutants nucleoporins and other Passenger Complex proteins.

Institute For Microbiology And Hygiene At The Charitè Berlin

Establishment and validation of an antibody based test (ELISA) for rapid detection of fungal infection in patients directly from patient material

Hamburg Area, Germany

Establishment of a method to trace YopE injected by Y. enterocolitica into human cells (primary HUVECs).

Doctor Of Philosophy (Ph.D.), Cell/Cellular And Molecular Biology

Master Of Science (M.Sc.), Biotechnology, Very Good

Diplom Ingenieur (Fh)/ Engineer Diploma, Biotechnology, Good