Anne Case Email & Phone Number

San Diego, California, US

San Diego, CA, US

San Diego, CA, US

San Diego, CA, US

La Jolla, CA, US

• Conducted project to quantify structure-function relationships among matrix metalloproteinases (MMPs) by comparing their cleavage efficiencies toward phage peptide substrates
• Orchestrated proteomics project to gain an understanding of how stress from tumor microenvironments and drug treatments can alter melanoma cell phenotype and lead to a drug resistant and invasive phenotype
• Performed substrate phage selections to arrive at test substrates and utilized ELISAs to quantify cleavage of phage substrates
• Proficiency with western blotting, SDS-PAGE, mass spectrometry, cell cultures (bacterial and mammalian), active site titrations, DNA library preparation for NGS, qPCR, AKTA protein purification, RNA/DNA extraction.

• Provided leadership and encouragement to high school girls interested in pursuing engineering careers
• Assisted course instructors and staff with projects, field sessions, tours, and other activities
• Developed and led icebreakers and team-building activities

• Performed research of immunogenetics at the Antczak Laboratory investigating the polymorphism and gene structure of the equine Major Histocompatibility Complex (MHC)
• Analyzed MHC class II genes across horse MHC haplotypes
• Assessed alleles of horses in research population identifying intra-MHC microsatellite markers to define MHC haplotypes
• Laboratory technique skills included polymerase chain reaction (PCR), gel electrophoresis, cloning, extraction/purification, restriction enzyme digestion, and analyzing sequences (next-generation and Sanger)
• Utilized GeneMarker, PeakScanner, and Geneious software to analyze DNA
• Developed comprehensive skillset in principles and procedures of laboratory operation and maintenance

New Haven, CT, US

• Tested hypothesis that β-cell death rates can identify those likely to develop type 1 diabetes
• Utilized assays developed by the Herold Lab to measure levels of methylated and unmethylated INS DNA in serum to determine rates of β-cell death employing droplet digital PCR (ddPCR) and tested new assays for improved.
• Conducted initial steps to sequence the INS gene products to determine whether there are methylation marks in the INS gene that identify β-cell stress
• Orchestrated DNA extraction and bisulfite conversion, quantified DNA and assessed purity using NanoDrop, prepared ddPCR reactions, and performed droplet generation, PCR amplification, droplet reading, and ddPCR data.
• Gained significant experience using QX200 ddPCR system and QuantaSoft software as well as the fluorescence-activated cell sorting (FACS) type of flow cytometry
• Performed cloning reactions and transformations, picked colonies, and analyzed the sequences

Ithaca, NY, US

• Supported laboratory research projects and assisted in diagnosing diseased plant samples
• Performed DNA extraction, PCR, and gel electrophoresis and prepared media for growing fungal cultures
• Completed laboratory maintenance duties including autoclaving items, washing lab equipment, and instrument care