Anne Case Email & Phone Number

San Diego, California, Us

San Diego, Ca, Us

San Diego, Ca, Us

San Diego, Ca, Us

La Jolla, Ca, Us

• Conducted project to quantify structure-function relationships among matrix metalloproteinases (MMPs) by comparing their cleavage efficiencies toward phage peptide substrates• Orchestrated proteomics project to gain an understanding of how stress from tumor microenvironments and drug treatments can alter melanoma cell phenotype and lead to a drug resistant and invasive phenotype• Performed substrate phage selections to arrive at test substrates and utilized ELISAs to quantify cleavage of phage substrates• Proficiency with western blotting, SDS-PAGE, mass spectrometry, cell cultures (bacterial and mammalian), active site titrations, DNA library preparation for NGS, qPCR, AKTA protein purification, RNA/DNA extraction, Bradford protein assay, primer design, and phage display

• Provided leadership and encouragement to high school girls interested in pursuing engineering careers• Assisted course instructors and staff with projects, field sessions, tours, and other activities• Developed and led icebreakers and team-building activities

• Performed research of immunogenetics at the Antczak Laboratory investigating the polymorphism and gene structure of the equine Major Histocompatibility Complex (MHC)• Analyzed MHC class II genes across horse MHC haplotypes• Assessed alleles of horses in research population identifying intra-MHC microsatellite markers to define MHC haplotypes• Laboratory technique skills included polymerase chain reaction (PCR), gel electrophoresis, cloning, extraction/purification, restriction enzyme digestion, and analyzing sequences (next-generation and Sanger)• Utilized GeneMarker, PeakScanner, and Geneious software to analyze DNA • Developed comprehensive skillset in principles and procedures of laboratory operation and maintenance• Co-author of abstract submitted for the ISAG 2017 conference in Dublin titled "Studies of MHC Class II content in three common Arabian horse haplotypes"

New Haven, Ct, Us

• Tested hypothesis that β-cell death rates can identify those likely to develop type 1 diabetes• Utilized assays developed by the Herold Lab to measure levels of methylated and unmethylated INS DNA in serum to determine rates of β-cell death employing droplet digital PCR (ddPCR) and tested new assays for improved results• Conducted initial steps to sequence the INS gene products to determine whether there are methylation marks in the INS gene that identify β-cell stress• Orchestrated DNA extraction and bisulfite conversion, quantified DNA and assessed purity using NanoDrop, prepared ddPCR reactions, and performed droplet generation, PCR amplification, droplet reading, and ddPCR data analysis• Gained significant experience using QX200 ddPCR system and QuantaSoft software as well as the fluorescence-activated cell sorting (FACS) type of flow cytometry• Performed cloning reactions and transformations, picked colonies, and analyzed the sequences • Utilized GraphPad Prism and Microsoft Excel software to analyze data and documented laboratory work

Ithaca, Ny, Us

• Supported laboratory research projects and assisted in diagnosing diseased plant samples• Performed DNA extraction, PCR, and gel electrophoresis and prepared media for growing fungal cultures• Completed laboratory maintenance duties including autoclaving items, washing lab equipment, and instrument care