Antu Thomas Email & Phone Number

Jülich, North Rhine-Westphalia, Germany

Institute Of Bioprocess And Biosystems Engineering, TUHH, Hamburg, Germany.

• Master Thesis title: Construction of a non-natural metabolic pathway for the production of 1,2,4 butanetriol, a high-value compound, from L-homoserine in Escherichia coli
• Thermodynamic feasibility study of the de novo pathway using the Group Contribution method.
• Cloned heterologous genes via restriction digestion/ligation or In-Fusion cloning.
• Site-directed mutagenesis, protein purification and its analysis (SDS-PAGE, concentration assay) and enzyme activity assay.
• Enzyme-coding genes co-expressed in E. coli strain which was genomically edited using CRISPR/Cas9.
• Fermentation using 1.5 L Dasgip® bioreactor and shake flasks to validate the de novo pathway.

Institute For Solids Process Engineering And Particle Technology, TUHH, Hamburg

• Produced lignin powders via spray drying and milling processes.
• Analyzed the particle size distribution of lignin powders produced.

Institute Of Technical Biocatalysis, TUHH, Hamburg, Germany.

• Title: Development and validation of a sampling technique combining offline and inline analytics in reactive distillation
• Kinetic resolution of racemic ethyl 3-hydroxybutyrate achieved using immobilized CalB enzyme in a reactive distillation column.
• Simultaneous inline ATR-FTIR and offline GC measurements were made in the reflux liquid collected at various column heights.
• ATR-FTIR coupled with the chemometric model continuously deduced the concentrations at different column heights.
• The correctness of the deduced concentrations was evaluated using offline GC measurements.

Thiruvananthapuram Area, India

Responsible for testing the input/output and processing unit of Xelerate ™ - the pricing and billing software from SunTec™ Business Solutions* Identification of test scenarios based on client requirements and developing test cases.* Peer review of test scenarios identified by the team members.* Execution of test cases, identifying and reporting of the.

Master'S Degree, Chemical And Bioprocess Engineering

Bachelor Of Technology - Btech, Biotechnology