• Maintenance and characterization of disseminated AML PDX bank, target mining and identification, target validation • Discovered and developed a novel serum biomarker for efficacy assessment in AML PDX models• Screened novel linker drugs using cytotoxicity assays in AML cell lines. • Supported solid tumor indications by screening for heme tox concerns

• Detection and quantification of key protein expression levels with and without treatment with test compounds- Homogenized lung tissue and extracted protein lysates. - Ran ELISAs for detection of protein expression levels in the different study groups. - Analyzed data and presented the same at weekly group meetings• Cell based assay to monitor inhibitory drug potency- Cultured primary mouse alveolar epithelial cells. - Stimulated with cytokine of interest and pre-treated with inhibitory drugs to check potency levels of different drug candidates. - Analyzed data and presented the same at weekly group meetings

1) The Mouse Ear: A new model system to study hair cycle regulation:My main project during my time at the Plikus lab. I carried out: - performing procedures on mice under anaesthesia- tissue collection and imaging (Gross imaging and Fluorescent microscopy)- EdU pulse-chase experiments- EdU assay development and optimization2) In-vivo hair characterization studies using mouse models- breeding and handling in vivo mouse models- sample collection and characterization- sample imaging and measurement- data collection and analysis- presentation of analyzed data- collaboration with the Mathematics department for mathematical modeling and statistical analysis 3) Optimization of dermal and epidermal cell collection from Mouse back skinThis mini-project was carried out using a special LEF-RFP/K14-H2B-GFP mouse model> Mouse tissue collection (back skin)> Tissue processing, to obtain single cell suspension> FACS to separate out the dermal and epidermal components of skin4) Trained undergraduate students in the essential techniques required for the above projects

As a teaching assistant, I handled three different courses:• Two graduate level laboratory courses for Molecular Biology and Stem cell Biology• One undergraduate level lecture course on AIDSFor the graduate level courses, I was responsible for:• Training students in fundamental techniques of Molecular Biology and Stem Cell Biology, as well as stem cell culture maintenance• Preparing and planning for the lab, setting up experiments, organizing schedules, preparing and grading in-lab quizes and pre- and post- lab meetings• Coaching students on experimental data analysis, report writing and giving presentations Molecular biology:> DNA Digestion, Ligation> Plasmid vector preparation> DNA Gel Electrophoresis> Transformation> SDS-PAGE> Protein expression and purification (E. Coli)> HPLC (basics)> MS (basics) Stem cell culture maintenance:> mESC, hESC, iPSC> feeding, culturing, splitting, passaging> feeder and feeder-free culture methods> maintenance in undifferentiated state> differentiation> transfection, nucleofection> immunocytochemistry > imaging (bright-phase, fluorescent)For the Undergraduate course, I was responsible for:• holding office hours for the students• administering and grading tests

During my time at Allergan, I was mainly involved in the following three projects:1) Gene Expression Studies Testing Novel Ophthalmic Compound - Cell Culture of primary human RPE cells and stable transformed RPE cell line (RPE-19) - RNA extraction and cDNA preparation from cells treated with compound - qPCR to measure gene expression of cAMP responsive genes - qPCR data analysis and interpretation2) In vivo Experiments Using Wild-Type and Knockout Mice Testing Compounds For Photoreceptor Protection - Mouse model using bright light to induce photoreceptor damage - Measured damage induced by light both visually by Heildelberg Retinal Angiograph (HRA) for auto-fluorescence and Electro-Retinal Angiogram (ERG) for functional response. - Whole eye enucleation for histological analysis, posterior eye-cup preparation and retina dissection for other studies.3) Performed experiments Testing Novel Dermatology Drug - In vivo drug testing using Golden Syrian Hamsters for effect on the sebaceous gland - Prepared drug prior to treatment - Quantitative measurement of dermal thickness response after drug treatmentPresented the results and progress of these projects to the Vice-Presidents of Allergan and other team members at the monthly meeting

• Trained in and carried out the techniques of micropropagation: > Plant media preparation> Culture Initiation> Culture Establishment> Culture Multiplication> Culture Packaging for export• Trained in rigorous aseptic technique to prevent contamination

Trained in > understanding SOPs> Environmental monitoring> Clean room etiquette> Filter integrity testing (basics)> HPLC and its application in Extractable Analysis> Filtration(using the various Millipore equipment)> Protein estimation using BCA> SDS PAGE> Luminex (multi-ELISA technology)

• Assisted in teaching Bioinformatics• Prepared notes and study material for the class