Anushka Gupta Email & Phone Number

Pleasanton, California, Us

Pleasanton, California, Us

Berkeley, Ca, Us

• Developed a microfluidic workflow, μCB-seq, for paired imaging and RNA-sequencing of single-cells. Optimized sequencing chemistry for improved gene detection sensitivity while reducing library preparation costs by 50x. Automated the entire operational workflow using laboratory robotics.• Implemented computational and statistical methods to reveal transcript enrichment and detection biases in single-nuclei RNA-seq as compared to single-cell RNA-seq. Developed a novel normalization framework to account for said detection biases while analyzing sequencing datasets.• Designed and performed multiplexed, time-course, snRNA-seq experiments (~ 100,000 nuclei) to study human adipogenesis. Developed a sample preparation workflow for efficient isolation of intact nuclei from fragile specimens. Established an end-to-end bioinformatic pipeline for the analysis of acquired high-dimensional snRNA-seq datasets.

Vancouver, British Columbia, Ca

• Designed and fabricated a digital microfluidic platform for on-demand patterning of cells using dielectrophoresis • Characterized the performance of this platform for cell trapping time, and viability as a function of feature size

Blackfriars, London, Gb

• Assessed the Juice Intensification & Coating technology for sensory enhancement of tea bags • Provided key inputs on tea manufacturing and processing line for Juice Intensification trials at Assam, India• Evaluated novel tea coating technology based on speed of infusion, spectral measurement, and taster’s score

Doctor Of Philosophy - Phd, Bioengineering

B.Tech. (Honours) And M.Tech. In Chemical Engineering, Chemical Engineering

High School, Physics, Chemistry And Mathematics

High School, Science