Alan O'Neill Email & Phone Number

London, Gb

Oxfordshire, England, United Kingdom

o Establishing Dermatology as a new strategic focus area and translating existing capabilities into an integrated Dermatology platform to pitch new clients and partners. o Engaging directly with pharma and academic clients to develop and cost drug discovery programs, working in close collaboration with internal BD, pharmacology, chemistry, and DMPK colleagues across global Evotec sites.o Team Leader with direct reports and matrix team members - incorporating new technologies, improving… Show more o Establishing Dermatology as a new strategic focus area and translating existing capabilities into an integrated Dermatology platform to pitch new clients and partners. o Engaging directly with pharma and academic clients to develop and cost drug discovery programs, working in close collaboration with internal BD, pharmacology, chemistry, and DMPK colleagues across global Evotec sites.o Team Leader with direct reports and matrix team members - incorporating new technologies, improving automation of assays, and developing new complex skin models to attract new investment. o Biology Lead on several drug discovery programs - coordinating and supervising matrix work on various assays involving macrophage polarization, T-cell activation/proliferation, and compound profiling. Show less

o Led the design, execution and interpretation of a MoA study, utilizing single cell analyses (scRNA-Seq, flow cytometry and microscopy) of human acne patients, revealing how a subset of dermal preadipocytes contribute to the pathophysiology of acne and resolution during retinoid treatment. o Advanced the clinical development of a skin biotherapeutic drug. Formulated and manufactured the live biotherapeutic product (LBP) under CGMP conditions and co-wrote an Investigation New Drug… Show more o Led the design, execution and interpretation of a MoA study, utilizing single cell analyses (scRNA-Seq, flow cytometry and microscopy) of human acne patients, revealing how a subset of dermal preadipocytes contribute to the pathophysiology of acne and resolution during retinoid treatment. o Advanced the clinical development of a skin biotherapeutic drug. Formulated and manufactured the live biotherapeutic product (LBP) under CGMP conditions and co-wrote an Investigation New Drug application to the FDA to initiate Phase I clinical trial in humans. Show less

San Diego

o Discovered new bacterial-derived antimicrobial peptides (AMP) by LC/MS, demonstrated efficacy and tolerance of the AMPs in vitro and in vivo infection models and conducted successful target ID studies.o Conceived, executed, and analysed two high-throughput in vitro screens of the human and animal microbiome that discovered novel bacterial isolates with antimicrobial and anti-inflammatory activity. o Established a new acne mouse model that models pimple-like lesions in response to… Show more o Discovered new bacterial-derived antimicrobial peptides (AMP) by LC/MS, demonstrated efficacy and tolerance of the AMPs in vitro and in vivo infection models and conducted successful target ID studies.o Conceived, executed, and analysed two high-throughput in vitro screens of the human and animal microbiome that discovered novel bacterial isolates with antimicrobial and anti-inflammatory activity. o Established a new acne mouse model that models pimple-like lesions in response to infection and colonisation by the human skin bacterium Cutibacterium acnes (C. acnes). o Collected skin swabs from acne patients and healthy individuals and generated a large library of C. acnes isolates that were screened for pro-inflammatory activity in primary human keratinocyte cells. o Combined genomic, transcriptomic and metabolomic analyses of Cutibacterium acnes strains to uncover novel virulence mechanisms associated with acne vulgaris and their mechanism-of-action.o Published several manuscripts, wrote successful grant applications and supervised undergrads, postgrads and dermatology residents. Show less

10901 N. Torrey Pines Rd. La Jolla, San Diego

• Characterized important mediators of the antiviral immune response against HIV gp120 glycoprotein in the brain of various WT and TLR knockout transgenic mouse models• Optimized conditions for difficult assays on primary murine cerebrocortical cells for HIV infection • Applied my expertise in advanced confocal microscopy to image neuronal damage in mouse brain tissue

London, United Kingdom

• Marie Curie PhD scholarship to conduct original research project on virulence mechanisms of the skin pathogen Group A Streptococcus (GAS).• Developed advanced microscopy skills to quantify and report first direct evidence of intracellular survival and replication of GAS in the cytosol of human macrophages• Characterized several GAS mutant strains in the activation and/or escape of autophagy• Investigated the role of toxin-antitoxins in GAS persister formation to antibiotic-induced… Show more • Marie Curie PhD scholarship to conduct original research project on virulence mechanisms of the skin pathogen Group A Streptococcus (GAS).• Developed advanced microscopy skills to quantify and report first direct evidence of intracellular survival and replication of GAS in the cytosol of human macrophages• Characterized several GAS mutant strains in the activation and/or escape of autophagy• Investigated the role of toxin-antitoxins in GAS persister formation to antibiotic-induced stress.• Generated expertise in primary mammalian tissue culture, bacterial mutagenesis, molecular genetics, flow cytometry, microscopy and various infection and inflammatory in vitro assays• Obtained other transferable skills through the Imperial PhD Professional Development Programme and several Marie Curie workshops in Vaccinology, Leadership and scientific communication • Active on several collaborations that included a 2 month lab rotation at Karolinska Institutet, Sweden Show less

Dublin 4, County Dublin, Ireland

• Full time lab Research Masters in the Infection Biology Programme / Original research project involved finalizing the development and validation of a recombinant MUC5AC and MUC2 mucin model system in gastric and intestinal cells. • Conducted experiments to assess the intracellular trafficking and secretion of mucins, and the immunoprecipitation and purification of mucins.• Experimentally tested and validated binding of Helicobacter phylori and Campylobacter jejuni strains to the… Show more • Full time lab Research Masters in the Infection Biology Programme / Original research project involved finalizing the development and validation of a recombinant MUC5AC and MUC2 mucin model system in gastric and intestinal cells. • Conducted experiments to assess the intracellular trafficking and secretion of mucins, and the immunoprecipitation and purification of mucins.• Experimentally tested and validated binding of Helicobacter phylori and Campylobacter jejuni strains to the relevant MUC5AC and MUC2 mucins. Show less

Doctor Of Philosophy (Ph.D.), Microbiology And Immunology

Activities and Societies: Boxing, football and running

Master’S Degree, Mucosal Biology

Activities and Societies: Football, MicroSoc, Infection Biology journal club, tutor for undergraduate problem based learning (PBL) classes.

Bachelor’S Degree, Microbiology, General, 1St Class Honours (Gpa 3.9)

Activities and Societies: Football, Tennis