Ashley Brianne Palete Email & Phone Number

•Independently test antibody and buffer products on human blood, mouse tissue, and cell lines in a BSL-2 laboratory•Isolate peripheral blood mononuclear cells (PBMCs) from human blood•Culture PBMCs, mouse tissue, and cell lines•Stain samples via direct staining, indirect staining, intracellular staining, and transcription factor staining protocols•Perform flow cytometry using BD CantoII, BD LSRII, and BD LSRFortessa•Perform data analysis using FCS Express… Show more •Independently test antibody and buffer products on human blood, mouse tissue, and cell lines in a BSL-2 laboratory•Isolate peripheral blood mononuclear cells (PBMCs) from human blood•Culture PBMCs, mouse tissue, and cell lines•Stain samples via direct staining, indirect staining, intracellular staining, and transcription factor staining protocols•Perform flow cytometry using BD CantoII, BD LSRII, and BD LSRFortessa•Perform data analysis using FCS Express software•Independently completed an individual project for the Stability Team in testing heat accelerated stability of antibodies compared to real-time antibodies Show less

•Assist in in vivo, ex vivo, and in vitro experiments studying T cell exhaustion in mouse melanoma, mesothelioma, and pancreatic cancer, and chronic LCMV infection in mouse models by utilizing techniques in genotyping, phenotyping, ELISA, focus forming assays, metabolic Seahorse assays, cell culturing, tissue processing, flow cytometry, antibody staining, directional cloning, and retroviral transfection and transduction. •Perform lab mouse handling techniques such as scruffing, tumor… Show more •Assist in in vivo, ex vivo, and in vitro experiments studying T cell exhaustion in mouse melanoma, mesothelioma, and pancreatic cancer, and chronic LCMV infection in mouse models by utilizing techniques in genotyping, phenotyping, ELISA, focus forming assays, metabolic Seahorse assays, cell culturing, tissue processing, flow cytometry, antibody staining, directional cloning, and retroviral transfection and transduction. •Perform lab mouse handling techniques such as scruffing, tumor measurements with a caliper, subcutaneous tumor injections, intravenous tail injections, intraperitoneal injections, retroorbital blood collection, and harvesting of tumors, spleens, and lymph nodes.•Assist in mouse colony maintenance with breeding setups, phenotyping, and genotyping.•Analyze, organize, and present data to peers using Microsoft Excel and Microsoft Powerpoint. •Develop experimental and inventory maintenance-related SOPs.•Contribute to a small team of scientists in a fast-paced environment utilizing record-keeping, time management skills, excellent communication, collaboration, and keen attention to detail. Show less

San Diego

•Assisted in experiments studying T cell exhaustion in cancer with mouse models by utilizing PCR, gel electrophoresis, cell culturing, and directional cloning techniques.•Independently generated YUMM1.5-OVA melanoma cell line.

San Diego

Worked as a cashier in a team-oriented environment in order to provide efficient service to customers.

Professional Science Master’S In Biotechnology

Bachelor Of Science - Bs, Biochemistry And Cell Biology

Activities and Societies: Asian Pacific Health Foundation, Pre-Pharmacy Society, Phi Sigma Pi National Honor Fraternity, Multi-Asian.

Education record

Activities and Societies: California Scholarship Federation, UNICEF, Acts of Random Kindness Club, Marching Band, Symphony Orchestra.