Aprille (Matthews) Seidel Email & Phone Number

Homestead, Fl, Us

Miami, Fl, Us

Miami-Fort Lauderdale Area

Miami Fl

Running clinical trials for 510(k) clearance and PMA of medical devices. Writing study protocols, plans and procedures, monitoring sites, data labeling, data auditing, data analysis, post analytical reports

Designed and ran two clinical studies examining the immune responses to flu vaccination in stem cell transplant patients. CD4 and CD8 T cell responses measured by intracellular cytokine staining and flow cytometry. Interacted with CDC and WHO to obtain reagents needed for Influenza A and B hemagglutination inhibition assays. Maintained and managed the Flow Cytometry Core Facility for the Division of Translational Research. Work directly in BSL-2+ bio-containment facilities daily. Direct supervisor of two employees.; Completion of HIV and defensin graduate research. Responsible for establishment and maintenance of BSL-3 laboratory space. Trained incoming personnel to work safely in BSL-3 facility. Worked with institutional bio-safety officer to set up SOP for BSL-3. Worked in BSL-3 laboratory on a daily; Completed thesis on HIV-1 inhibition by alpha, beta and theta defensinsWorked in BSL-3 level facility on a daily basis; for Disease Control and Prevention, Laboratory for Retroviral Research, Research FellowWorked in BSL-3+ facility with a multitude of human and animal viruses. Work was focused on Porcine Endogenous Retrovirus, feline retroviruses, and simian retroviruses. Responsible for ordering and stocking supplies for the entire laboratory

Intermediate Cell Biology (Bio 303) Class size 25, Full teaching portfolio review and evaluation on file with the department

Completion of HIV and defensin graduate research. Responsible for establishment and management of BSL-3 laboratory space. Created HIV viral cultures from laboratory and clinical isolates. Worked directly with patient and healthy donor blood samples

Completed thesis work on HIV-1 inhibition by alpha, beta and theta defensins. Laboratory techniques utilized include: primary and transformed cell culture, flow cytometry, virus culture, isolation, purification, and real time quantitative PCR.Worked in BSL-3 level facility on a daily basis; Centers for Disease Control and Prevention, Laboratory for Retroviral Research, Research FellowWorked in BSL-3+ facility with a multitude of human and animal viruses. Work was focused on Porcine Endogenous Retrovirus (PERV), feline retroviruses, and simian retroviruses. Developed a western blot assay for the detection of PERV in human sera (see patents and publications). Culture and isolation of zoonotic viruses from individuals with positive antibody responses

Microbiology laboratory, wrote the supporting lab manual for the class, lab size 25 students

Worked on designing a western blot assay and screening samples for porcine endogenous retrovirus after xenotransplantation.

Research and development of commercial EIA kits for the detection of various antigens including auto immune markers and viral pathogens (measles, mumps, rubella, CMV, etc.

Studied the impact of cocaine use in HIV infection of brain microglia cells. Worked in BSL-3 on a daily basis. Cultured HIV from primary isolates. Cultured laboratory strains of HIV. Worked with controlled substances

Ph.D., Microbiology And Immunology

B.S., Microbiology And Immunology

Maiden name Matthews