Training, troubleshooting and consulting on Agilent Genomics solutions.

Head of department of Molecular Diagnostics, and coordinator for Serology, Medical Microbiology, Water Testing and Parasitology. Certified as an internal auditor in ISO 15189 and ISO 17025. Familiarity with the entire process of DNA extraction both automated and manual, as well as real Time PCR using different platforms. In charge of planning and performing verifications of CE-IVD workflows and writing Standard Operating Procedures.

Specialized in Illumina next-generation library preparation and sequencing (certified as an FAS in MiSeq, NextSeq, MiniSeq, amplicon library prep specialist by Illumina Inc, and experience in enrichment workflows) and other molecular biology applications. Familiarity with use, training and troubleshooting of molecular biology workflows. Familiarity with NA extraction, liquid handling applications, and liquid biopsy procedures. Routine use and troubleshooting of capillary electrophoresis, in conventional PCR and as quality control for library prep in NGS applications. Familiarity with real-time PCR detection and some service procedures.

Biological data mining of the Cancer Cell Line Encyclopedia for the discovery of cancer-related genes by epigenetic mechanisms other than methylation. Analysis of images by means of fluorescence microscopy videos of the NF-kB pathway, segmentation and tracking of individual cells. Lecture at the Latin American Congress of Biomedical Engineering (CLAIB 2014)

Determination of the in vitro effect of Rutaceae essential oils on cholesterol levels through the mevalonate metabolic route.Use of all-trans retinoic acid as chemopreventor of lesions induced by DNA alkylating agents and partial hepatectomy in rats. Standardization of an animal model of gastric cancer in rats, using n-methyl-n-nitrosoguanidine by intra-gastric cannulation, and detection of the lesions by histological techniques, both immunohistochemistry and conventional histology. Study of the proteomics of fish of the Scorpaenidae family, by means of gel digestion and mass spectrometry (MALDI-MS) (thesis project). Purification of the toxins present in the crude extract, by means of gel permeation chromatography, ion exchange chromatography, affinity chromatography and reversed phase HPLC (RP-HPLC), and determination of the cell death mechanism by flow cytometry.