Arlene Sutherland Email & Phone Number

San Diego, California, United States

Boston, Massachusetts, USA

• Led the technical portion of the DARPA “Friend or Foe” grant:
• Designed, executed, and analyzed experiments where the goal was to use the human Colon Chip to identify unknown pathogens
• Successfully co-cultured human intestinal cell lines with pathogenic and non-pathogenic bacteria On Chip with multiple readouts and endpoints
• Planned the strategy and timelines for how to analyze samples of unknown pathogenic bacteria with multiple PIs at Harvard Medical School to meet time-sensitive milestones
• Oversaw the set-up of a BL2+ facility and ensured proper protocols were in place and followed by all users
• Presenter and Wyss representative at the DARPA Friend or Foe PI Meeting in Arlington, VA (2019)Microbiology Lead on the Gates Foundation grant funding the Vaginal Microbiome Research Consortium (VMRC):

Boston, Massachusetts, USA

Mcmaster University, Hamilton, Ontario

• Laboratory technician for molecular biology and microbiology undergraduate laboratories in the Department of Biology at McMaster University:
• Extensive preparation and organization of laboratory experiments for all departmental laboratory courses in Molecular Genetics, Molecular Biology, Cell Biology, and Microbiology
• Collaborated with faculty, staff and students to design, adapt, and modify course programs
• Designed and tested experimental methods for new undergraduate laboratory exercises
• Trained and mentored teaching assistants to ensure successful laboratory outcomes
• Procured consumables, reagents, and equipment, and effectively minimized operational costs

InfectionControl.tips, Hamilton, Ontario

A global collective of experts and non-experts creating a discussion about infection control and prevention using the open-access online publication, www.InfectionControl.tips.

Hamilton, Ontario, Canada

• Successfully created a natural product extract library (>5000 extracts) of fermentation products from over 3500 unique bacteria (primarily Actinobacteria) for use in High-Throughput Screening
• Designed, developed, and optimized the production of the screening library using bioassays, HPLC, and mass spectrometry to find ideal conditions for bioactive small molecule production
• Assisted in chemical structure elucidation of potential screening hits through culture fermentation, bioassays, and large-scale compound production
• Conducted operational cost analysis of the project and ensured adherence to budget constraints
• Managed the organization, storage, and distribution of all compound library plates
• Collaborated with the McMaster Stem Cell and Cancer Research Institute (SCC-RI) as part of the Ontario Consortium for Regeneration Inducing Therapeutics (OCRit)

Ancaster, Ontario, Canada

Mcmaster University, Hamilton, Ontario, Canada

• Graduate research in the laboratory of Dr. Gerry Wright:
• Worked towards developing a well-curated library of cloned antibiotic resistance genes for use in High-Throughput Screening for novel antibiotics and inhibitors of antibiotic resistance
• Constructed a series of inducible reporter vectors in E. coli and B. subtilis for cell-based screening
• Conducted antimicrobial susceptibility assays for evaluation of resistance profiles
• Worked extensively with solid and liquid bacterial cell culture, and bacterial growth assays
• Designed PCR primers, programs, and methods to amplify bacterial genes, as well as manipulate bacterial DNA and introduce mutations

Mcmaster University, Hamilton, Ontario, Canada

• Held tutorials for a 3rd year biochemistry class, Metabolism and Regulation
• Guided 2nd year students through an intense biochemistry lab course, Inquiry in Biochemical Techniques, and taught students new theory and techniques
• Responsible for assessing students based on in-class performance and formal written reports

Mcmaster University, Hamilton, Ontario

• Research assistant, and undergraduate thesis student (BIOL 4C09), in the laboratory of Dr. John Hassell:
• Responsible for cell culture and maintenance of both primary cells and established cells lines, specifically mammary epithelial stem cells, while ensuring proper cell density and viability
• Isolated mammary glands and mammary gland tumors from viable mice, and characterized murine mammary tumor growth
• Compared the morphology and histology of serially passaged mouse mammary tumors using light microscopy
• Performed immunohistochemistry assays on sections of isolated murine mammary glands and mammary tumors
• Analyzed cell surface markers of mammary epithelial cells and mammary stem cells using fluorescence microscopy and imaging software.

Mcmaster University, Hamilton, Ontario

• Highschool co-op student in Dr. Patricia Chang’s laboratory:Supervisor: Dr. Pasquale Cirone
• Cultured cells, performed literature searches, and observed gels, 5 days a week for 3 hours per day with the help and guidance of a supervisor
• Attended weekly journal club meetings and lab meetings, and presented a journal article in one of the meetings
• Learned how to make solutions, use aseptic technique, set up and run ELISAs, culture mammalian cells, and use laboratory equipment such as centrifuges and pH meters

Hamilton, Ontario, Canada

Activation Laboratories, Ancaster, Ontario

• Prepared organic samples for GC/MS/MS analysis
• Organized, weighed and diluted soil samples

Master Of Science, Biochemistry And Biomedical Sciences

Thesis: Construction of Cell-Based Antibiotic Resistance Arrays Courses: Protein Crystallography, Mechanism of Enzyme Action

Honors Bachelor Of Science, Biology - Genetics Specialization

Undergraduate Thesis Supervisor: Dr. John Hassell, Director of the Centre for Functional Genomics Thesis: Comparison of the Cellular.

Math And Sciences

Activities and Societies: Chess clubOntario Scholar Award, 2002

Education record