Project: In-silico Study of Phytoconstituents as an alternative for Anti-Cervical Cancer Drugs - Gathered information on phytoconstituents from various plant sources with documented anti-cancer properties. Compiled a diverse dataset, including molecular structures and known bioactivities of selected phytoconstituents. - Conducted an extensive review of existing literature on cervical cancer, molecular targets, and mechanisms of anti-cancer phytoconstituents. Identified relevant proteins and pathways associated with cervical cancer progression. - Retrieved three-dimensional (3D) structure of target protein involved in cervical cancer using Protein Data Bank (PDB). - Prepared ligand structures of phytoconstituents. Optimized ligand conformations and minimized energy to ensure accuracy in the docking simulations. - Employed molecular docking techniques to predict the binding affinity and modes of interaction between phytoconstituents and target proteins. Utilized software tool AutoDock for efficient ligand-protein docking simulations. - Selected lead phytoconstituents based on docking results for further investigation through molecular dynamics simulations. Conducted simulations to explore the dynamic behavior and stability of ligand-protein complexes over an extended period. - Analyzed the binding sites of target proteins to understand the specific interactions with phytoconstituents. Investigated the potential of the identified binding sites for drug development. - Assessed the Absorption, Distribution, Metabolism, Excretion, and Toxicity (ADMET) properties of lead phytoconstituents. - Analyzed docking scores, molecular dynamics trajectories, and ADMET predictions. - Selected phytoconstituents with favorable properties for further consideration as potential anti-cervical cancer drug candidates.

*Worked on diverse samples of Salix species, representing various geographical locations and environmental conditions. Ensured inclusion of both common and lesser-known species for a comprehensive analysis.*Employed standardized DNA extraction protocols to obtain high-quality genomic DNA from collected samples.*Utilized molecular markers such as microsatellites (SSRs) and single nucleotide polymorphisms (SNPs) to assess genetic diversity.*Conducted Polymerase Chain Reaction (PCR) amplification using selected molecular markers.*Analyzed PCR products using gel electrophoresis.

Focused on acquiring knowledge of diverse lab equipment and mastering various biochemical techniques, including SDS-PAGE, Western Blotting, Immunohistochemistry, and Flow CytometryActively involved in data collection for research purposes, showcasing a commitment to detail-oriented and systematic work.