• Developed primary cells from biopsies synovium and investigated molecular targets of various differentially expressed proteins.• Identified various proteins and evaluated their role in disease pathogenicity and progression, including validation at the mRNA level.• Maintained a cell culture research facility and conducted molecular biology experiments, including ELISA, Western blot, immunoprecipitation, SDS-PAGE, LC-MS/MS, and SWATH analysis using cell lysate. • Interact with patients to obtain consent and conveyed information regarding their participation in the study. Additionally, assisted clinicians for case studies and GAIT analysis.

• Conducted synthetic drug screening to detect gene-level alterations and validated the findings through siRNA transfection using the H9C2 cell line as a model for cardiovascular disease.• Performed macroscopic analysis of cardiac cell hypertrophy using NIS element software and confocal microscopy.• Tested various candidate synthetic drugs for their toxicity in cardiomyocytes.• Evaluated apoptosis and autophagy markers through FACS and fluorescent microscopy on iCell beating cardiomyocytes to assess the efficacy of the candidate synthetic drugs in managing cardiac hypertrophy.• Investigated the effects of insulin on apoptosis and autophagy at the gene and protein levels using an immunoproteomics approach and RT-PCR.

• Conducted a differential proteome profiling of synovial fluid and plasma samples from patients and compared them with samples from healthy volunteers to identify disease-associated proteins using iTRAQ labelling and LC-MS/MS.• Carried out screening of highly differential proteins to identify potential diagnostic and prognostic markers.