Ashley Rouleau Email and Phone Number

Associate scientist on the proteomics team at Johnson & Johnson Innovative Medicine (contracted through Kelly BPO).

Leveraged liquid handling automation to carry out daily DNA Synthesis operations, which included: amplification and error correction of constructs, enzymatic assembly of plasmids, molecular cloning of plasmids in competent E. coli DH5α cells, purification of plasmid DNA Ran team’s internal NGS pipeline (Illumina Miseq) to identify perfect sequences for customers Designed and conducted experiments to improve yield of challenging orders, identify sources of failure, and expand the limits of the DNA Synthesis pipelineTracked, catalogued, and created physical backups of all incoming DNA ordered by program teams from a third-party vendor, and worked with the vendor and internal software team to troubleshoot and improve the receiving systemTrained new operators across multiple foundry services and teamsCoordinated with the NGS operations team and executed their service pipelines (Illumina Novaseq) to maintain the service schedule and return to pre-pandemic capacity