 Heading industrial and academic projects in mammalian and stem cell culture e.g., In vitro cytotoxicity and DMPK studies, cell isolation and expansion, Caco-2 and Blood brain barrier models, skin irritation assay on 3D tissue models like SkinEthic RHE (Episkin) adhering to GLP, OECD and ISO 10993 guidelines. Purifiying antibodies and proteins like IgG, hyaluronic acid, albumin, asparaginase, and peptides (Jelleins). o Performing upstream processes such as mammalian cell and microbial fermentation for protein production at lab scale (5L to 50L), and carrying out transient and stable gene expression in CHO & HEK293 cell lines. o Downstream processes such as fractionation and purification of proteins by depth filtration, ultrafiltration, tangential flow filtration (Pellicon, Merck Millipore) and chromatography (Bio-Rad NGC systems).  Flow cytometry assay and panel design, execution, and data analysis for research assays and clinical tests adhering to ICCS & CLSI guidelines. Gene expression analysis by RT-qPCR, protein analysis and detection by SDS-PAGE, western blotting, and protein quantification by ELISA, BCA, and Bradford method. Bioinformatics analysis in RStudio & Galaxy e.g., RNAseq data analysis (MultiQC, STAR, Salmon, DESeq2, GO, KEGG, data visualization), Microbiome R package & QIIME2 metagenomic analysis of microbiome.  Analysis and interpretation of analytical data from GC/LC-MS, ICP-MS, and Spectrometry. Developed COVID-19 Viral Transport Medium (IVD). Headed the production & scale up during the COVID-19 pandemic.  Ensuring sterility of clean rooms, equipment calibration, cGMP compliance, and QMS (ALCOA+, ISO 9001:2015 & ISO 13485:2016). Statistical data analysis using GraphPad prism, Minitab, and RStudio. Collaborating with research heads from various companies and institutions.  Management responsibilities including work distribution, team monitoring, training, development and evaluation of employees.

 Animal Cell Culture based assays Flow cytometry protocol optimization & data analysis qPCR, Nucleic acid isolation & quantification Conducting training & workshops in the above mentioned fields