-Generated murine melanoma tumor model to study mechanisms of resistance to immunotherapy-Investigating mechanisms tumors engage to evade immunotherapy induced drug responses -Investigating combination therapy to increase the efficacy of checkpoint blockade immunotherapy-Performing preclinical characterization of new immunotherapy drug targets as a part of the drug development team at MD Anderson’s ORBIT program-Identified mechanisms of checkpoint blockade immunotherapy induced adverse effects

-Observed and studied adverse effects of immunotherapy on cancer patients in clinical trials-Learned preclinical trial designing for oncoimmunology drugs

-Supported the development, qualification and validation of methods to characterize a drug product for patients with Hemophilia A (Recombinant Porcine FVIII)-Performed analytical testing in support of process development studies for drug substance/drug product and stability programs-Used SEC-HPLC to determine the concentration, aggregation and degradation of drug substance/drug product-Characterized the different charge variants of rp-FVIII using AEX-HPLC-Purified protein using AKTA Avant and UNICORN software-Packed affinity column to purify and characterize impurities -Determined product potency with One Stage Coagulation Assay (OSCA) and Chromogenic Assay (CHROM)-Prepared technical documents and write reports for internal distribution and inclusion in regulatory filings

- Developed and optimized multiplex RT-PCR assays - DNA and RNA isolation and purification - Maintained bacterial culture - Designed and conducted experiments RT-PCR, qPCR- Worked in BL-1 & BL-2 laboratories in GLP settings- Analyzed results and generate conclusions

Supervisor- Dr. Deana Molinari(Fellowship from Rural Nurse Residency Program)-Analyzed research data using statistical software, -Designed experiments, -Prepared and edited research reportsPublications:Deana L Molinari, Ashvin Jaiswal, Tamara Hollinger (2010). Rural Nurses: Lifestyle Preferences and Education Perceptions. Online Journal of Rural Nursing and Health Care. D. L. Molinari, A.R. Jaiswal, T. Peterson (2011). Rural Nurse Perceptions and Organizational Culture. Rural Nurse Transition to Practice.

Laboratory- Dr. James C. K. Lai - Executed daily operations of maintaining neuronal cell culture.- Designed and conducted research experiments.- Total DNA/RNA extraction and purification, RT-PCR, SDS- PAGE, flow cytometer and enzymology.- Managed laboratory inventory, and monitored the quality of a team's work.- Mentored undergraduate students.- Analyzed research data using statistical software.- Prepared and presented research data at national and international meetings.PostersAshvin Jaiswal, Alok Bhushan, Christopher K. Daniels, James C.K. Lai. A Cell Culture Model for Pathophysiologic Studies of Diabetic Neuropathy, 2010 Peripheral Nerve Society Satellite Meeting. Peripheral Nervous System Satellite Meeting, July, 2010, Sydney, Australia. http://onlinelibrary.wiley.com/doi/10.1111/j.1529-027.2010.00277.x/abstract Ashvin Jaiswal, Yin Yin W. Wong. Vikas V. Dukhande, and James C.K. Lai. Neuroprotective Factors Secreted by U-87 Astrocytoma (Astrocytes-Like) Cells and Their Associated Metabolic Adaptation. Experimental Biology FASEB, April, 2010, Anaheim, CA. http://www.fasebj.org/cgi/content/meeting_abstract/24/1_MeetingAbstracts/763.6OralsAshvin Jaiswal, Yin Yin W. Wong, Alok Bhushan, Christopher K. Daniels and James C.K. Lai. A Non-contact Co-Culture Model of Peripheral Neural Cells for Nanotoxicity, Tissue Engineering and pathophysi- ological Studies. NSTI Nanotech, June, 2010, Anaheim, CA. http://www.nsti.org/procs/Nanotech2010v3/8/X32.402Ashvin Jaiswal, Alok Bhushan, Christopher K. Daniels and James C.K. Lai. The Cell Culture Model and Pathophysiology of Diabetic Neuropathy. Idaho Academy of Science Symposium, March, 2010, Twin Falls, Idaho. (Third Prize)