Proficient in conducting molecular diagnostic tests for UTI, STI, COVID, and RTI panels using advanced technology like TaqMan Array and Open Array.Ensured strict compliance with CLIA guidelines, leading comprehensive testing initiatives for multiple panels and achieving precise and reliable results.Demonstrated expertise in managing diverse testing panels, while employing Quant Studio 7 Flex and Kingfisher RNA/DNA Extraction instruments for proficient sample preparation and extraction.Maintained instrument proficiency, guaranteeing accurate sample processing, and enhancing overall laboratory efficiency.Spearheaded the implementation of robotic platforms using Lynx and Hamilton devices, enhancing the laboratory's high-throughput capabilities and diagnostic efficiency. Oversaw the setup and optimization of robotic systems, streamlining the testing process and improving overall efficiency. Successfully integrated robotics to boost the laboratory's testing capacity, significantly increasing the speed and accuracy of diagnostic procedures. Played a pivotal role in elevating the laboratory's operational efficiency, leading to improved diagnostic throughput and enhanced accuracy in test results.

Devised high throughput cloning techniques to address extraction challenges of lasso peptides from Streptomyces, leading to notable improvements in process efficiency. Constructed over 500 plasmid analogs to identify optimal LAS-103 analogs for mouse Endothelin B-receptor, leading to enhanced binding and process efficiency with positive outcomes.Successfully scaled and optimized cloning processes for diverse lasso peptide analogs targeting Integrin receptors.Developed cutting-edge high-throughput screening methods and analog libraries, including cloning of up to 100K member library, for efficient screening of large numbers of analogs.Achieved notable impact through strategic peptide engineering, creating E. coli variants with enhanced binding to αvβ6/αvβ8 receptors, showcasing expertise in molecular biology.Achieved enzyme evolution in lasso peptide machinery to improve analog binding, overcoming production limitations. Expanded analog screening options, paving the way for platform development, and showcasing expertise in enzyme evolution, protein purification, and phage display.Utilized LC-MS methods for extraction, isolation, and analysis of lasso peptides from small-scale bacterial fermentation broths, showcasing advanced analytical skills and playing a crucial role in the patent filing process for lasso peptide analogs.

Molecular cloning - Successfully designed and constructed more than 100+ complex plasmid builds utilizing plasmid extraction methods, RE digests, PCR/RT-PCR, Gibson/T4 ligation, Bacterial Transformation, DNA sequencing analysis, and quality assurance to produce the desired plasmid that will be ready for downstream C.elegans/ Zebrafish Microinjections. C. elegans transgenesis/phenotyping - Successfully completed more than 70+ projects in designing/constructing, and genotyping of C. elegans which was optimized to the client needs by several genome editing methods such as CRISPR/c as9, MosSCI, and Extrachromosomal array. In addition to that, worked in 5+ assay development projects, HRMA genotyping, RT-PCR, lifespan analysis, and worm husbandry. Zebrafish transgenesis - Professtionally Trained at the University of Oregon in several zebrafish husbandry processes, such as setting up fish crosses, fin-clipping, bleaching embryos, Fluorescence screening, and embryo injections. Involved in the transgenic molecular screening of 50+ projects CRISPR/c as9 edits, Tol2 edits, sgRNA designs, bioinformatics analysis, custom injection mix preparation, and DNA sequencing preparation/analysis. Successfully worked in setting up fish rack systems from scratch to a fully operational one. Marketing - Successfully wrote 5 technical blogs (few yet to be published) published on the company website, newly developed assay protocol, and completed around 12 technical company-wide presentations focussed on building the marketing content for the company website landing new projects, attracting new customers to improve the overall company visibility, and productivity.

Molecular cloning - Independently design complex plasmid builds, plasmid preparations, RE digests, PCR/RT-PCR, Gibson/T4 ligation, Bacterial Transformation, DNA sequencing analysis, and quality assurance to produce the desired plasmid that will be ready for downstream C.elegans/zebrafish Microinjections/Zebrafish microinjections. C. elegans - Involved in designing, constructing, and genetically editing C. elegans which was optimized to the client specifications by several genome editing methods such as CRISPR/cas9, MosSCI, and Extrachromosomal array. Transgenics were performed by deleting, mutating, or replacing endogenous content via CRISPR gene editing. In addition to that, worked in assay development projects, lifespan analysis and worm husbandry.

Completed extensive regulatory affairs training in the validation part of Pharmaceuticals and medical devices, analyzing the QA systems, regulatory writing, CMC sections of INDs, CMC documentation, and Database setup.

Completed a research proposal on Plant tissue culture which was selected among National level candidates by Indian Academy of Sciences and worked as a summer research fellow in the plant biotechnology laboratory.Utilized the full potential of various techniques, such as somatic embryogenesis, shoot bud induction, shoot proliferation, rooting, acclimatization, and micropropagation. Within three months, we can able to engineer the Dianthus plant species.