Product and method development researcher working on early product clone selection mutation analysis by mutation enhanced HRM and confirmation sequencing. Automation and process development to decrease hands on time and sample to sample variability in variant analysis workflow and anger sequencing.Sequencing product DNA in support of genetic consistency section for BLA filings, developed CE method for Sanger sequencing of short amplicon fragments, automated cherry picking of variant samples, learned and implemented VBA to revise an excel macro used to translate HRM output data to automation commands.

Researcher in the PCR lab of the Protein Analytical Chemistry process research and development group. Validation of products going to Phase 1 trials to BLA and detection of mutations by PCR. Worked with quality and ICH guidlines to design, perform, and report on validations on clinical products. Supported BLA submissions with product characterization by DNA sequencing of identity. Developing methods to detect unknown variants at low levels (<1%) in product clones.Authored Protocols/Reports/SOPs, quality critical reagents for CRIM, assess 454 NGS for mutation detection, Safety Inspection Team leader for department, qualified qPCR instrumentation for next gen rodent parvovirus detection qPCR assay, Sequenced product DNA in support of genetic consistency section for BLA filings, Coordinated across four departments to write new PC/PV protocol for use in BLA sequencing.

QC associate in the PCR lab of the Protein Analytical Chemistry group of ADQC. Method validation of products going to Phase 1 trials to BLA filing. Coordinated the design and relocation move of the GMP PCR lab. GMP method validation, GMP training and safety compliance, Review QC logs and lab equipment compliance, reagent qualifications, assay troubleshooting of primer dimers from Taq and primer comparisons, characterization of PTP plasmid, record keeping, communicate with QC network and testing groups, 5S of the PCR lab, DMS's closed in a timely manner and kept to a minimum, authored and revised SOPs, planned and managed department off-sites.

QC associate in the PCR lab of the Protein Analytical Chemistry group of ADQC. Method validation of products going to Phase 1 trials to BLA filing.GMP method validation, GMP training and safety compliance, Review QC logs and lab equipment compliance, reagent qualifications, assay troubleshooting, record keeping

Functional analysis of a gene family by engineering inducible siRNA, transforming, and inducing to study the gene family. Previous work was using bioinformatics approaches to find gene family candidates and engineer working siRNA.PCR, RNAi, NA extraction and purifications, DNA sequencing, primer design, bacterial and plant transformation, molecular cloning, gene mutagenesis, recombinant DNA by RE, aseptic techniques, electrophoresis.

Instructor of intro biology lab teaching basic science and biology techniques and skills.Taught critical thinking and lab skills, scientific methodology, team-oriented problem solving, completed yearlong teacher partnership program in pedagogical studies,

Customer service and employee training.

Prepared stock solutions and media, organized lab space, cleaned and kept stock of glassware and equipment, supported molecular and intro biology skill training, and ran experiments that had time constraints within class time.

Analysis of gene products in bread mold due to different carbon sources. Grew mini and maxi preps for DNA, RNA, and protein extraction and purification for studies.

Gave primary care to big cats, ranging from hands on training to feeding to enclosure care.