Astor Ankney Email & Phone Number

Durham, North Carolina, Us

• Apply bio-analytical techniques such as HPLC/UPLC (IEX, SEC, RP), capillary electrophoresis, HIAC, and other biophysical characterization techniques on proteins to monitor physical and chemical stability. • Participate in product development and QC efforts including testing to support method transfer, development, qualification, product characterization, and routine GMP and non-GMP analysis.• Record information, facts, and measurements accurately, clearly, and consistently as required by cGxP.

Durham, Nc, Us

• Prepared plasma, fecal, and tissue samples for quantitative analysis of short-chain fatty acids and other metabolites • Implemented a single injection method for the quantitation of an expanded panel of fatty acids and organic acids• Operated a Waters Acquity liquid chromatography system and Waters Xevo TQ-S mass spectrometer in multi-reaction monitoring (MRM) mode• Performed Quality Control (QC) tests to ensure data integrity and reproducibility

Research Triangle Park, Nc, Us

• Identified post-translational modification site variance between wild-type and mutant protein using affinity enrichment and liquid chromatography-mass spectrometry (LC-MS)• Optimized protein expression in labeled media for use in nuclear magnetic resonance structural experiments• Optimized purification conditions for a protein with atypical biophysical properties for analysis by cryo-electron microscopy

Chapel Hill, Nc, Us

• Optimized protein identification and quantitation by adjusting existing methods for label-free quantitative mass spectrometry • Streamlined biomarker discover by developing a novel approach which links secreted protein data from human cell lines to existing clinical databases • Analyzed large proteomic datasets using a variety of software tools including MaxQuant, Perseus, Ingenuity IPA, String-db, and FunRich • Processed both cell line and tissue samples for mass spectrometric analysis • Performed cotransfection, immunoprecipitation, and chemiprecipitation for the identification of novel interactors

Chapel Hill, Nc, Us

• Taught dry lab sections of introductory biochemistry (BIOC 107L & 108L) to classes of approximately 40 students each.

Greenville, Nc, Us

• Extracted medicinal compounds from Ban Lan Gen (Isatis indigotica root) using subcritical water • Optimized HPLC separation of phenol mixtures • Determined parabens solubility in subcritical water • Cloned, expressed, and purified recombinant proteins using E coli systems • Characterized interaction between HTLV-1 encoded protein HBZ and DNA helicase RecQL5 using common biochemistry techniques • Assayed histone acetyltransferase activity of transcriptional coactivator p300 in vitro

Research Triangle Park, Nc, Us

• Mapped the epitope of a single chain variable fragment antibody against cockroach allergen Bla g 1 using molecular biology techniques including PCR and ELISA.

Seattle, Wa, Us

• Optimized efficient movement of >100 trucks between 12 mills and 9 nonfixed logging sites based on quotas and available resources • Rapidly altered plans based on unexpected delays or breakdowns• Communicated effectively with drivers and site managers from widely differing backgrounds

Columbia, Maryland, Us

• Located truck transportation and negotiated rates to maximize profit margin for equipment moves across the US and Canada under strict deadlines• Managed multiple projects simultaneously through excellent multi-tasking and workload planning

Doctor Of Philosophy (Phd), Biochemistry

Bachelor Of Science (Bs)/Bachelor Of Arts (Ba), Biochemistry/Chemistry

Bachelor Of Science (Bs), Political Science