Astor Ankney Email & Phone Number

Durham, North Carolina, US

• Apply bio-analytical techniques such as HPLC/UPLC (IEX, SEC, RP), capillary electrophoresis, HIAC, and other biophysical characterization techniques on proteins to monitor physical and chemical stability.
• Participate in product development and QC efforts including testing to support method transfer, development, qualification, product characterization, and routine GMP and non-GMP analysis.
• Record information, facts, and measurements accurately, clearly, and consistently as required by cGxP.

Durham, NC, US

• Prepared plasma, fecal, and tissue samples for quantitative analysis of short-chain fatty acids and other metabolites
• Implemented a single injection method for the quantitation of an expanded panel of fatty acids and organic acids
• Operated a Waters Acquity liquid chromatography system and Waters Xevo TQ-S mass spectrometer in multi-reaction monitoring (MRM) mode
• Performed Quality Control (QC) tests to ensure data integrity and reproducibility

Research Triangle Park, NC, US

• Identified post-translational modification site variance between wild-type and mutant protein using affinity enrichment and liquid chromatography-mass spectrometry (LC-MS)
• Optimized protein expression in labeled media for use in nuclear magnetic resonance structural experiments
• Optimized purification conditions for a protein with atypical biophysical properties for analysis by cryo-electron microscopy

Chapel Hill, NC, US

• Optimized protein identification and quantitation by adjusting existing methods for label-free quantitative mass spectrometry
• Streamlined biomarker discover by developing a novel approach which links secreted protein data from human cell lines to existing clinical databases
• Analyzed large proteomic datasets using a variety of software tools including MaxQuant, Perseus, Ingenuity IPA, String-db, and FunRich
• Processed both cell line and tissue samples for mass spectrometric analysis
• Performed cotransfection, immunoprecipitation, and chemiprecipitation for the identification of novel interactors

Chapel Hill, NC, US

• Taught dry lab sections of introductory biochemistry (BIOC 107L & 108L) to classes of approximately 40 students each.

Greenville, NC, US

• Extracted medicinal compounds from Ban Lan Gen (Isatis indigotica root) using subcritical water
• Optimized HPLC separation of phenol mixtures
• Determined parabens solubility in subcritical water
• Cloned, expressed, and purified recombinant proteins using E coli systems
• Characterized interaction between HTLV-1 encoded protein HBZ and DNA helicase RecQL5 using common biochemistry techniques
• Assayed histone acetyltransferase activity of transcriptional coactivator p300 in vitro

Research Triangle Park, NC, US

• Mapped the epitope of a single chain variable fragment antibody against cockroach allergen Bla g 1 using molecular biology techniques including PCR and ELISA.

Seattle, WA, US

• Optimized efficient movement of >100 trucks between 12 mills and 9 nonfixed logging sites based on quotas and available resources
• Rapidly altered plans based on unexpected delays or breakdowns
• Communicated effectively with drivers and site managers from widely differing backgrounds

Columbia, Maryland, US

• Located truck transportation and negotiated rates to maximize profit margin for equipment moves across the US and Canada under strict deadlines
• Managed multiple projects simultaneously through excellent multi-tasking and workload planning

Doctor Of Philosophy (Phd), Biochemistry

Bachelor Of Science (Bs)/Bachelor Of Arts (Ba), Biochemistry/Chemistry

Bachelor Of Science (Bs), Political Science