Ardian Soca Wibowo, Ph.D. Email & Phone Number

Indianapolis, Indiana, United States

Indianapolis, Indiana, United States

Website: www.helixbiostructures.comThe typical drug development pipeline consists of several phases. Here at Helix BioStructures, we specialize in the early drug discovery phase and provide a dedicated focus in therapeutic discovery, physical characterization, protein synthesis, and related software development. The discovery phase of drug development centers on investigating small molecules (traditional pharmaceuticals or drugs) and protein (large molecules) based therapies such as antibodies. Our expertise encompasses drug design and development, chemical and structural analysis, protein production and purification, and computational modeling and design. This knowledge base allows us to assist you in several ways:1. A full suite of structural biology resources to analyze and determine the composition and configuration of therapeutic molecules2. In-house production facilities to synthesize proteins with a given specificity and purity3. Computational protein-ligand docking and analysis to aid in the elucidation and refinement of drug design

Greater Chicago Area

Shamrock Structures, LLC is a CRO located in the Greater Chicago Area that specializes in drug discovery research services for pharmaceutical researchers. Research services include: (1) CrystalEXPRESS, (2) StructureEXPRESS, (3) MedChemEXPRESS, (4) CellBioEXPRESS.

Indiana University, Bloomington, Indiana

As a Facilities Director of both the Macromolecular Crystallography Facility (MCF)/ Crystallography Automation Facility (CAF) and the Physical Biochemistry Instrumentation Facility (PBIF), I am an experienced research scientist in both chemical biology and biochemistry, with expertise in structural biology and X-ray crystallography techniques for soluble and membrane proteins. The MCF/CAF was founded to facilitate and encourage research endeavors in protein X-ray crystallography and structural biology. Managing this facility has allowed me to gain and strengthen skills in implementing advanced high-throughput automation robotics in crystallization experiments. In fact, one of my main roles as facilities director of the MCF/CAF is to maintain the integrity of robotic function and reliability. I have performed screening utilizing the ARI Phoenix DT/RE and performed optimization with the Rigaku Alchemist DT. With the ARI Crystal Gryphon LCP, I helped researchers implement experiments toward crystallization of membrane protein utilizing lipidic cubic phase. Automated imaging/inspection occurred with the Rigaku Minstrel HT and I aided researchers in analysis with the CrystalTrak application. Subsequently, I have experience utilizing a home source Rigaku RU-H2R R-Axis IV X-Ray generator as well as remote access to Beamline 4.2.2 at the Advanced Light Source (ALS) for screening and data collection My knowledge of the instruments and research allow me to aid and train users in order to provide a centralized resource for training and education in latest crystallographic and structural biology techniques. MCF Website: www.indiana.edu/~iumcfPBIF Website: www.indiana.edu/~physbio

Bloomington, Indiana Area

I developed the graduate course, Biophysical Analysis of Macromolecules, offered to junior graduate (1st/2nd year) students. The objective of the class is to foster an understanding of the theory, application, and limitations of available instrumentation housed in the PBIF. These instruments are commonly used to solve questions inherent to many biochemical/biophysical research endeavors. These are invaluable but underutilized approaches to simply and rigorously characterize macromolecular interactions, multimeric status of complexes, order of assembly of macromolecules and rates of important processes. Students taking the course benefited in having direct hands-on exposure to each instrument covered through practical experiments. In addition, having such experience will aid in familiarity to the PBIF (as well as the MCF) and knowledge of experimental capabilities inherent to instruments covered within the course. Experiences in the class through practical exposure may hopefully be extrapolated to usage and implementation of instruments by students in their research endeavors toward the completion of thesis projects.

Bloomington, Indiana Area

As an adjunct lecturer, I taught four semesters of Anatomy and Physiology to potential nursing students taking the course. Anatomy and Physiology is one of the most difficult classes to teach in a manner that aids students in the comprehension of the material. I was able to successfully teach the material in a means to help students enjoy the subject manner, comprehend the material, and prepare them for subsequent classes in the nursing degree. Through teaching the course, I have gained a better understanding in my teaching philosophy where the instructor must make every effort to provide any means necessary so that students have the advantage in learning the material of the course.

Bloomington, In

The work as a research associate involves analysis and characterization of human folate receptors (hFR) by taking the information gained from the solved/refined structural models and applying these insights to mutant studies of the receptor. Key interactive residues in ligand binding have been identified through my doctoral work to achieve atomic resolution structures of hFRs in states that mimic folate uptake and biological trafficking for both folates and antifolates. The mutant constructs were designed and expressed transiently and in mammalian Chinese hamster ovary (CHO) cell lines. These mutant hFR proteins were characterized through biophysical means such as ITC to evaluate binding affinity compared to wildtype protein. These mutants will also be structurally characterized in comparison to our available FR structural models in hopes of improving rational drug design based on structural information rather than strictly on empirical principles for increased efficacy in chemotherapeutics.

Dann Research Group, Department Of Chemistry, Indiana University

Indiana University• Successfully completed cloning, overexpression, and purification of folate receptors (FR) alpha and beta by tissue culture involving both insect (sf9 and HiFive) and mammalian (CHO) cells• Crystallized, collected data, built and refined structures of FR proteins to obtain apo and complex states with natural folate ligands and various antifolate drugs• Synchrotron experience at the Advanced Photon Source (APS) at Argonne National Labs• Used remote robot access to screen and collect diffraction data at the Advanced Light Source (ALS), Molecular Biology Consortium (MSC) 4.2.2 beamline, Berkeley, CA• Performed biophysical binding characterization of Folate Receptors using Isothermal Titration Calorimetry (ITC), intrinsic and fluorescence anisotropy, and surface plasmon resonance to probe protein-ligand and protein-drug interactions• Experience with Microscale Thermophoresis• Experience with Phoenix Automated Crystallization robotics• Assisted in the training of graduate research students• Assigned and oversaw laboratory duties and maintenance of laboratory equipment• Frequently presented and discussed research progress in group as well as departmental settings• Worked collaboratively with members of other research groups

Bloomington, In

• Trained at least six undergraduate and a number of graduates• Taught students basic and advanced laboratory skills, including preparation and handling of reagents, synthetic reaction procedure, and troubleshooting, and TLC and column chromatography for monitoring and purification of reactions• Introduced students to molecular biology skills, such as cloning and DNA manipulation, bacterial and tissue culture, protein expression and purification, and gel electrophoresis• Explained techniques of protein crystallography including spares matrix screening, crystal growth optimization, freezing, X-Ray diffraction, data collection, model building, and structure refinement• Helped students develop technique in crystal manipulation and data collection while using X-Ray sources at both Argonne National Labs, Chicago, IL, and the Advanced Light Source, Berkeley, CA.

Bloomington, In

Courses: 1. Macromolecular Structure and Interaction (C581) (Fall 2008)• Graduate course, aid to instructor • Contributed to students' comprehension of material, including, inter-and intro-molecular interactions, structural stability of proteins and nucleic acids, and thermodynamic and kinetic analysis of complex binding2. Intermediate Inorganic Chemistry Laboratory (N330) (Summer 2008)• Provided instruction in the fundamental techniques of inorganic and organic chemistry, including electrochemistry, transition metal and coordination chemistry, organo-metallic complexes, and bioinorganic chemistry• Graded assignments, reports, and tests as well as aided students with overall comprehension and analysis of laboratory material and experimental procedures3. Elementary Chemistry Laboratory I (C121) (Spring 2008)• Provided introduction to techniques and reasoning of experimental chemistry• Graded assignments, reports, and tests as well as aided students with overall comprehension and analysis of laboratory material and experimental procedures4. Principles of Chemistry and Biochemistry II Laboratory (C118) (Fall 2005 and Fall 2006)• Provided instruction for the integration of basic chemistry and biochemistry principles within context of important social, political, economic and ethical issues, including polymers, drug design, nutrition, and genetic engineering• Graded assignments, reports, and tests as well as aided students with overall comprehension and analysis of laboratory material and experimental procedures5. Principles of Chemistry and Biochemistry I Laboratory (C117) • Taught multiple sections in the Spring and Fall of 2006 and 2007• Provided instruction for the integration of basic chemistry and biochemistry principles, including applications to physiological (biochemical) functions• Graded assignments, reports, and tests as well as aided students with overall comprehension and analysis of laboratory material and experimental procedures

Bloomington, Indiana Area

My doctoral work solved the atomic resolution structures of human FRs (hFR) in states that mimic folate uptake and trafficking for both folates and antifolates. Membrane bound folate receptors (FRs) are cell surface glycosyl-phosphatidylinositol (GPI)-anchored glycopeptides. Two membrane bound FRs have been identified and characterized to date, FRα and FRβ. FRα is a tumor marker overexpressed in several cancerous malignancies of epithelial origin. Consequently, FRα is widely recognized as a potential target for the selective delivery of a broad range of therapeutic agents to tumors. FRβ is upregulated in activated macrophages involved in inflammatory disease. Physiological traits have made FRs a relevant drug vector due to high affinity binding and the potential to transport a chemically diverse range of folate conjugates, antifolate drugs, and immunological agents. Design of drugs for FR-mediated therapy has been entirely empirical and efficacy is still limited due to transport via the ubiquitously expressed reduced folate carrier (RFC), causing dose limiting toxicity to normal tissue. The structural models of my doctoral work are imperative to improving rational drug design based on structural information rather than strictly on empirical principles for increased efficacy in chemotherapeutics.

Doctor Of Philosophy (Ph.D.), Chemical Biology

Activities and Societies: American Crystallographic Association (ACA), Chemistry Graduate Representative Committee (ChemGRC).

Bachelor Of Science, Biochemistry, Cum Ladae

Activities and Societies: Alpha Chi Sigma Professional Chemistry Fraternity