• Assay development (planning, writing, and execution) for fast-paced client projects:o ddPCR for GOI confirmation and quantification, as well as for quantification of residual plasmid DNAo qPCR (bespoke and kit-based) for quantification of viral product genome and residual host-cell DNAo TCID50 for determination of AAV infectious titer via HeLa cell infection, lysis, and ddPCRo ELISA for product titer and residual host-cell protein measuremento Transgene expression by ELISA-based titration of protein of interest in supernatant from HEK293 cellso BacPAK for baculovirus infectious titer via insect cell inoculation and kit-based immunoassay• Sample testing of drug substance and in-process samples from process development and manufacturing labs• Analyzing and organizing data for efficient and effective science-driven communication• Leading project discussions and data presentations in virtual and onsite meetings with clients• Coordinating teams of analysts to complete sample testing priorities on rapid timelines• Improved proficiency with high-throughput workflows, utilizing 96-well assay plates• Writing and implementing qualification protocols and test methods for method qualification• Independently completed verification of a kit-based mycoplasma detection assay used for material entry testing

Teaching Specialist: Jan. 2022 – May 2022Graduate Teaching Assistant: Aug. 2021 – Dec. 2021• Instructed and supervised senior-level biochemistry students in execution and analysis of techniques, including:o Real-time (quantitative) PCRo Enzyme purification: ammonium sulfate precipitation; size exclusion and ion exchange chromatographyo Western Blots and ELISAo Enzyme binding and kinetics assayso UV-Vis and fluorescence spectroscopy• Successfully executed each experiment as planned by the professor, while adapting a new lab space for the course and troubleshooting unforeseen obstacles with minimal oversight• Developed skills in science communication: planned and presented detailed briefings of procedures and analysis before each exercise, in addition to one-on-one discussions in-person or via virtual meetings and email

• Developed a strategy to preferentially capture yeast proteins with nonspecific RNA-binding activity via affinity purification, and identified candidates for RNA chaperone activity by mass spectrometry of recovered proteins• Demonstrated the ability of yeast Lhp1 to unwind RNA duplexes and promote refolding of stable, misfolded RNA structures to native conformations via in vitro kinetic assays with recombinant protein• Determined equilibrium constants and kinetic parameters for nonspecific binding interactions between Lhp1 and radiolabeled RNA substrates via electrophoretic mobility shift and filter binding assays• Designed, cloned, transcribed, and validated constructs of the Tetrahymena ribozyme with combined mutations to maximally inhibit cis- and trans-cleavage activity while preserving native RNA structure

Aug. 2016 – May 2019; Jan. 2021 – May 2021 (7 semesters total)• Instructed and supervised senior-level microbiology students in execution and analysis of techniques, including:o Animal cell culture: BHK-21, NIH-3T3, and primary cell culture of chicken fibroblastso Cryopreservation and cell viability assessment via hemocytometry with vital staino Purification of virus by sucrose gradient centrifugationo Metabolic labeling and radiometric quantitation for transcription of viral RNA in cell cultureo Hemagglutination and hemagglutination-inhibition assays for virus particle and antibody titrationo Immunofluorescent staining of viral proteins to determine cellular localization and infectious viral titero Optical and fluorescence microscopy• Honed aseptic technique suitable for work with animal viruses and cell culture in a laminar flow hood

• Systematically assayed water samples to detect bacterial contamination• Organized data into a Laboratory Information Management System• Performed after-hours testing in response to emergencies• Maintained “Superior” rating for City of Houston drinking water

• Cloned Borrelia burgdorferi surface protein gene for recombinant expression• Purified recombinant protein for biochemical assays• Crystallized B. burgdorferi surface protein