Techniques: ✓ iPSCs (induced pluripotent stem cells) culture✓ iPSCs differentiation into neural stem cells and mature neurons✓ Compartmented culture on microfluidic devices✓ Confocal microscopy & inverted microscopy✓ Comet assay (DNA breakage assay)✻ Skills: ✓ Development of the protocole for the differentiation of iPSCs into mature neurons.✓ Development of the protocole for the reconstitution of a cortico-striatal network on microfluidic devices using Huntington disease mice.✓ Supervision of an engineering trainee for her capstone project: development of the internship project, supervision at the bench and during the internship dissertation.✎Project 1: Reconstitution of cortico-striatal network on a microfluidic device to study synaptic structure and transmission in Huntington’s disease.✎Project 2: Study of DNA damage and DNA repair in neural stem cell and medium spiny neuron derived from human Huntington disease iPSC.

During my Ph.D thesis, we highlighted a protein as a new biomarker for ischemic stroke. This postoctoral contract gives the opportunity to pursue and complete this project, a patent and a paper is currently in process.✻ Techniques: ✓ primary culture (neuron)✓ cell line culture (HEK)✓ molecular biology (qPCR, immunoprecipitation, Westernblot)✓ ELISA✻ Skills:✓ Build and manage a research project with collaborators.✓ Conception and design experiments.

✎Thesis project: Modulation of neuronal autophagy by tissue-plasminogen activator (tPA) in ischemia. ✻ Techniques: ✓ primary culture (neuron, astrocyte, microglia, oligodendrocyte)✓ cell line culture (HEK), molecular biology (qPCR, immunoprecipitation, Westernblot)✓ microscopy (confocal, inversé, STED)✓ ELISA✓ gene knock-down via lentivirus expressing sh-RNASkills:✓ Autonomy (bibliography, project and experiments design, results presentation)✓ Congress: ➺7th day of Autophagy (Paris, 2018, Poster presentation) ➺Gordon Research Conference on plasminogen activation and extracellular proteolysis (Ventura, USA, 2019, Oral prensentation) ➺Society for Neuroscience (San Diego, USA, 2019, Poster presentation).✓ Supervision: one undergraduate student and three masters student.✓ Designing, delivering and assessing practical and laboratory- based sessions (Master student).

✻ Techniques: ✓ primary culture (neuron, astrocyte, oligodendrocyte, microglia) ✓ cell line culture (HEK, SH-SY5Y)✓ pharmacology (inhibitors, dose/response)✓ apoptosis and excitotoxicity model✓ cellular death test✓ molecular biology (PCR, qPCR, immunoprecipitation, Westernblot)✓ proximity Ligation Assay. ✎ Project 1: Study of the pathway involved in the neuroprotection mediated by tissue-plasminogen activator (tPA) following OGD-induced ER stress. ✎ Project 2 : Study of the effect of sc-tPA and tc-tPA on the crosstalk between MET and NMDA receptor by proximity ligation assay technique.

✻ Techniques: ✓ cryostat✓ microtome✓ HES coloration✓ immunohistochemistry

✻ Techniques: ✓ confocal microscopy,✓ neuronal primary culture✓ molecular biology✎Project: Caracterisation of antibodies directed against single chain (sc-tPA) and two chain (tc-tPA) plasminogen activator.

✻ Techniques: ✓ primary culture✓ microfluidic culture✓ molecular biology (PCR, Westernblot)✓ fluorescence microscopy✻ Skills: ✓ Establishment of a new model to be proposed to clients✓ Autonomy (bibliography, experiments design, results presentation).✎Subject 1: Development of a microfluidic neuronal culture to study synaptic transmission in Alzheimer disease.✎Subject 2: Phenotypic screening of a chemolibrary (more than 200 molecules tested) on an in vitro neurodegenerative disease model.

✻Techniques: ✓ cryostat✓ microtome✓ HES coloration✓ immunohistochemistry