Research Scientist 1
Seattle, Washington, United States
Wet lab technician performing various next generation sequencing procedures, including: Metagenomic "shotgun" sequencing for whole genome recovery of high-target load specimens. Amplicon sequencing protocols implementing pre-existing and custom whole-genome tiling amplicon panels, and custom target region amplicon panels for specific species identification and mutation surveillance in regions of interest. Hybridization-capture protocols using custom hybridization probes and multi-target kits for whole genome recovery from low target concentration specimens.Workload included use of various laboratory machinery. Sequencing platforms used include Oxford Nanopore MinION, Ilumina NextSeq2000, NovaSeq6000 and MiSeq. Automated systems used in workflow included the PerkinElmer Sciclone G3, Roche MagNA Pure 96, and Thermo Scientific KingFisher. Other included technology includes QuantStudio 7, Applied Biosystems 7500, BioRad C1000 Touch, PerkinElmer Victor Nivo, and Agilent TapeStation.