Babu Ram Prasad Saravanan Email & Phone Number

United States

 Maintenance, expansion and cryopreservation of human iPSCs, iPSC derived neurons, and patient derived fibroblast. Differentiating iPSCs into different neural cell types using transcriptional reprogramming/small molecule approach and use them as disease models. Studying gene expression and neuronal cell characterization using qPCR and Immuno cytochemistry (ICC). Expertise in dissociating, maintaining, cryobanking and performing quality control testing of mouse glia and Schwann cells. Hands-on-experience in 384 well plate culturing. Proficient in using Viaflo. Designing and generation of recombinant expression plasmids using molecular biology techniques Planned and executed project critical cell culture experiments followed by its characterization using RT-qPCR. Documented the execution of studies in compliance to company standards and generated summary report of study results along with data analysis.  Trained team members with molecular biology and tissue culture skills.  Lab Operations: Was in-charge of molecular biology reagents for the entire team, amongst other responsibilities.

Billerica

 Trained in high throughput antibody development using B-cell cloning technique. Experienced in various PCR techniques like RT PCR, QPCR and nested PCR. Confident in handling automated instruments for high throughput mini DNA preparation, purification, re-arraying and colony picking Performed various ELISA techniques for assay development

Boston

Dr. Loring’s Lab at Northeastern University, Boston, MA, USA. Studying transmembrane Protein 35 (TMEM 35)’s and resistance to inhibitors of cholinesterase 3 (RIC 3) roll in α7 receptor assembly and functioning. Trained to edit these genes by designing PCR primers, followed by digestion ligation and transformation. Expert in maintaining animal cell lines. Studied α7 expression in HEK cells using radioactive α-Bungarotoxin (with I125) in a binding assay. Performed analytical SDS-PAGE followed by western blot for antibody screening. Assisted in iodination of α-Bungarotoxin.

Marketing division, Urology at Alembic Pharmaceuticals, India. Responsible for the sales and marketing of urology drugs in my territory. Experience communicating the details of my product to most prominent urologists in my territory. Performed market analysis and survey to understand the product potential

Chennai Area, India

HBioinformatics department at Sankara Nethralaya, India. Studied the mutations in low-density lipoprotein receptor-related protein 5 (LRP5) resulting in familial exudative vitreoretinopathy (FEVR), a rare eye disease and tried to generate the 3D structure using homology modelling (using Modeller) and further structure refinement using Ramachandran Plot. Analyzed the effect of pont mutation from different perspectives like conservation analysis (ConSurf and Panther), Disorder prediction (DisEMBL, GlobPlot, Ronn and Disopred) and other tools like SIFT, POLYPHEN, PMut were also used. Molecular dynamic studies of refined LRP5 structure was simulated (using GROMACS, Maestro Schrodinger) followed by molecular docking to epidermal growth factor 5 (EGF 5) (using auto dock). Interested in automation studies

Master Of Science - Ms, Biotechnology, 3.76

MS in Biotechnology at Northeastern University , has enabled me to not just understand Biotechnology better but has given me an insight.

Bachelor'S Degree, Biotechnology, 7.8

Completed Bachelor's in Technology in Biotechnology that helped me build a strong foundation in the fundamental concepts of Biotechnology.