Balaji Vasudevan, Ph.D. Email and Phone Number

Q: What is Balaji Vasudevan, Ph.D.'s email address?

Balaji Vasudevan, Ph.D.'s email address is vasudevanbalaji@gmail.com

Director of Programs at @ First Flight Venture Center

Chapel Hill, NC, US

Balaji Vasudevan, Ph.D.'s Location

Raleigh-Durham-Chapel Hill Area, United States, United States

Balaji Vasudevan, Ph.D.'s Contact Details

Balaji Vasudevan, Ph.D. personal email

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About Balaji Vasudevan, Ph.D.

Balaji Vasudevan, Ph.D.'s Current Company Details

First Flight Venture Center

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Director of Programs

Chapel Hill, NC, US

Website:: firstflightventure.org
Employees:: 39

Balaji Vasudevan, Ph.D. Work Experience Details

Director Of Programs

First Flight Venture Center

Chapel Hill, Nc, Us

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Agrifoodtech Freelance Expert

Foodtech Experts

Chapel Hill, Nc, Us

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Technical And Commercial Judge

Uae Foodtech Challenge Dec 2024 - Present

Abu Dhabi, Abu Dhabi, Ae

As a Technical & Commercial Judge in the UAE FoodTech Challenge, I am involved in critically evaluating innovative agri-food technology offerings developed by global early-stage agri-food tech start-ups with solutions related to the food-water-energy nexus.. Here’s a detailed description of my contributions:Evaluation: Assess technical proposals and projects based on criteria such as innovation, feasibility, scalability, and impact on the food industry.Scoring: Provide objective and detailed scores for each entry, ensuring a fair and balanced assessment process.Feedback: Offer constructive feedback to participants, helping them improve their solutions and presentations.Expert Guidance: Share your expertise in food technology to guide and support the development of cutting-edge solutions.Panel Deliberations: Participate in panel discussions to debate and select the most promising projects.Trend Analysis: Stay informed about the latest advancements in food technology to ensure that evaluations are current and relevant.Mentorship: Provide mentorship to participants, helping them navigate technical challenges and refine their innovations.Networking: Facilitate connections between participants and key industry stakeholders to foster collaboration and growth.About FoodTech ChallengeDrive forward the UAE’s food security. The competition is open to early-stage start-ups with tech-driven solutions poised to poised to transform food systems in arid, hot environments. Four winning teams will win a share of $2M equity-free cash and grants to incubate in the UAE and scale to underserved markets in the Global South 🌍Benefits:✈️ An expense-paid trip to the UAE to compete at the competition finale 💰 $2M in equity-free cash & grants for four winning teams consisting of$100K to accelerate your start-up $200K for local impact in the UAE $200K for global impact in the Global South 🌐 Access to a robust network of industry experts, accelerators, and partners

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Foodtech 500 Ambassador

Forward Fooding Nov 2024 - Present

London, London, Gb

Member of the FoodTech 500 Ambassador Program. FoodTech 500 is the world’s first Global FoodTech ranking and is a unique initiative that allows FoodTech businesses to showcase the amazing work they are doing on the global stage.AgriFoodTech startup nomination partner for the "FoodTech 500", an initiative by Forward Fooding.I nominate 10+ deserving AgriFoodTech startups from my global network of over 30K in the AgriFoodTech/Climate Ag/AgTech/Syn Bio/Regen Ag/Novel Foods/Crop Protection/Crop Improvement sector.FoodTech 500 has reached a global audience in 180+ countries, generated 2.5M+ social media impressions, and boasts a monthly readership base of 350M+ with 45+ media partners involved, and we have taken the list all over the world, through our 'Unveiling FoodTech 500' events gathering more than 2,000+ participants over the last few years.For more information about the initiative (and see the award-winnners from previous years):https://forwardfooding.com/foodtech500See the list of ambassadors here:https://forwardfooding.com/foodtech500/ambassadorsForward Fooding is the world’s first collaborative platform for the food & beverage industry fostering innovation via FoodTech Data Intelligence and corporate-startup collaboration.

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Mentor

Moonshot Pirates Oct 2024 - Present

Vienna, At

Empowering Tomorrow's Innovators in synthetic biology, agtech, agrifoodtech, novel foods, food and agriculture sector for sustainable food innovation and climate change mitigation. Excited to be a part of growing movement that develops young people between 15 and 20 to think big, learn, and act toward a better future. Mentoring to equip young innovators to create solutions to real-world problems.As a mentor at Moonshot Pirates, you inspire and guide young innovators and entrepreneurs to transform bold ideas into impactful ventures.Activities:Visionary Guidance: Provide strategic advice and insight to help mentees develop their ideas into viable projects or businesses.Skill Enhancement: Conduct workshops and training sessions to impart essential entrepreneurial skills such as leadership, pitching, and business planning.Network Building: Connect mentees with relevant industry experts, investors, and stakeholders to expand their network and resources.Project Evaluation: Offer constructive feedback on project plans, presentations, and prototypes, ensuring they meet high standards of innovation and feasibility.Inspirational Leadership: Motivate and encourage young entrepreneurs, fostering a mindset of resilience, creativity, and relentless pursuit of their goals.Resource Provision: Guide mentees in accessing and utilizing available resources and tools that can help accelerate their projects.Continuous Learning: Stay abreast of emerging trends and innovations to provide relevant and up-to-date mentorship.

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Agrifoodtech Thought Leader

Agrinext Conference Aug 2024 - Present

Greenbelt, Maryland, Us

As an AgriFoodTech Thought Leader at the AgriNext Conference, I play a pivotal role in shaping the future of agriculture and food technology through insightful discussions, innovative ideas, and thought-provoking presentations.Activities:Keynote Speaker: Deliver engaging and visionary keynote presentations that highlight the latest trends, challenges, and innovations in the AgriFoodTech sector.Panel Discussions: Participate in and moderate panel discussions with industry experts, fostering dynamic conversations on critical topics.Workshops and Seminars: Lead workshops and seminars to share deep insights and practical knowledge on cutting-edge technologies and sustainable practices.Networking Facilitator: Connect with fellow thought leaders, innovators, and stakeholders to build meaningful relationships and collaborations.Advisory Role: Provide strategic advice and guidance to startups, researchers, and industry professionals on the future direction of AgriFoodTech.Publication and Research: Contribute to whitepapers, reports, and articles that disseminate valuable knowledge and research findings to a broader audience.Mentorship: Mentor aspiring entrepreneurs and researchers, offering your expertise and support to help them succeed in the AgriFoodTech landscape

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Operations And Program Managers

Kickstart Venture Services Feb 2024 - Present

Working with Faculty Founders/Innovators in UNC Chapel Hill at North CarolinaIn my current role, I have the opportunity to work and collaborate with academic innovators and faculty founders in universities, non-profits, research institutes. I coach, mentor and support academic innovators to translate ideation to ventures, build startups by 1) Pitch deck help2) Connection to VCs and Investors3) Provide grant awards to de-risk POC and build MVP4) Coach innovators to be investor ready5) Data room build help6) Connection to grant writing vendors to submit SBIR/STTR proposals for securing non-dilution funding7) Expose innovators to advisory group in RTP area. The group members are experienced in startup development, regulatory, BD, startup operations, VCs, Consultants and other expertise.8) We also provide wet lab space of R&D9) Organize and provide opportunity for faculty innovators by organizing Venture Day and exposing startups to VCs and help pitch 10) Connection to scaling partners 11) Worked with other University partners to organize VC Day to help startups pitch to VCs in multiple verticals12) Help faculty innovators help fund raise (pre-seed/seed)13) Connect innovators with industry partners

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Venture Partner

16 Fund Jan 2024 - Present

New York, Ny, Us

Deal Sourcing and Evaluation: Utilize your network and industry knowledge to identify high-potential investment opportunities. Assess and evaluate potential investments based on Loading Fund's criteria.Due Diligence: Conduct thorough due diligence on potential investment targets, collaborating with our investment team to ensure comprehensive analysis.Portfolio Management: Work closely with portfolio companies, providing strategic guidance and leveraging your experience to help them achieve their full potential.Networking: Build and maintain strong relationships within the startup ecosystem, fostering connections that will benefit both Loading Fund and our portfolio companies.

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Mentor

California Life Sciences (Cls) Sep 2023 - Present

South San Francisco, Ca, Us

As a mentor at California Life Sciences, I guide and support emerging biotech and life sciences startups, fostering innovation and growth within the industry.Activities:Strategic Advisory: Provide high-level strategic advice to startups, helping them define their vision, mission, and business goals.Business Development: Assist startups in developing robust business models, identifying market opportunities, and crafting effective go-to-market strategies.Technical Guidance: Offer expertise in scientific and technical areas, ensuring startups can navigate complex challenges and make informed decisions.Networking and Connections: Leverage your extensive network to connect startups with potential investors, industry experts, and key stakeholders.Workshops and Training: Conduct workshops and training sessions to equip founders with essential entrepreneurial skills and knowledge.Feedback and Evaluation: Participate in pitch sessions, providing constructive feedback to refine business plans and presentations.Continuous Learning: Stay updated with industry trends and advancements to provide relevant and up-to-date mentorship.Emotional Support: Encourage and motivate founders, fostering a positive and resilient mindset through the ups and downs of startup life.

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Navigator/Mentor

First Flight Venture Center Aug 2023 - Present

Durham, North Carolina, Us

Strategic Guidance: Provide expert advice on business strategy, market positioning, and growth plans.Mentorship: Offer personalized mentorship to founders, helping them navigate challenges and make informed decisions.Networking: Connect startups with potential investors, partners, and industry experts to expand their network.Resource Access: Facilitate access to essential resources such as lab space, equipment, and funding opportunities.Workshops and Training: Conduct workshops and training sessions on key topics like fundraising, regulatory compliance, and product development.Feedback and Evaluation: Participate in pitch sessions, providing constructive feedback to refine business plans and presentations.Continuous Learning: Stay updated with industry trends and advancements to provide relevant and up-to-date guidance by providingEmotional Support: Encourage and motivate founders, fostering a positive and resilient mindset through the ups and downs of startup life.

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Mentor

Big Idea Ventures Jul 2023 - Present

New York, Ny, Us

As a mentor at Big Idea Ventures, I play a pivotal role in guiding and nurturing innovative startups within the food and agribusiness sectors.Activities:Expert Guidance: Provide strategic advice and insights based on your industry expertise to help startups navigate challenges and seize opportunities.Business Development: Assist startups in developing robust business models, identifying market opportunities, and crafting effective go-to-market strategies.Networking Support: Leverage your professional network to connect startups with potential partners, investors, and key stakeholders.Skill Development: Offer hands-on mentoring in critical areas such as finance, operations, marketing, and product development.Feedback and Evaluation: Participate in pitch sessions, provide constructive feedback, and help refine business plans and presentations.Continuous Learning: Stay updated with industry trends and emerging technologies to provide relevant and up-to-date guidance.Emotional Support: Encourage and motivate founders, fostering a positive and resilient mindset through the ups and downs of startup life.

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Mentor For Agtech Startups

Unsung Heroes Acceleration Programme Jun 2023 - Present

Warsaw, Mazowieckie, Pl

Unsung Heroes Acceleration Programme is an international space that connect humanitarian organizations and startups. There is one mission to stop gobal hunger using technology. There are 27 startups form 19 countries who are supported by mentors from 43 countries. Mostly WaterTech, AgroTech and HealthTech solutions. More information at unsung.tech. As a mentor, I provide Strategic Guidance: Provide comprehensive strategic advice on business models, market entry strategies, and scaling operations to help startups navigate the competitive Agtech landscape.Technical Expertise: Share your deep knowledge of agricultural technology and innovations, guiding startups in the development and implementation of cutting-edge solutions.Networking and Connections: Leverage your extensive network to connect startups with potential investors, strategic partners, and industry experts, facilitating valuable collaborations and funding opportunities.Educational Workshops: Lead workshops and training sessions on critical topics such as sustainable farming practices, regulatory compliance, and technological advancements in Agtech.Feedback and Evaluation: Participate in startup pitch sessions, offering detailed and constructive feedback to refine business plans, prototypes, and presentations.Resource Access: Help startups access essential resources, including funding, technology, and market insights, to accelerate their growth and development.Continuous Support: Provide ongoing mentorship and support, offering advice and encouragement to help founders overcome challenges and achieve their goals.Innovative Thinking: Encourage innovative thinking and problem-solving, inspiring startups to explore new ideas and approaches in the Agtech sector.Impact Assessment: Assist startups in measuring and communicating the social and environmental impact of their innovations, emphasizing the importance of sustainability and community engagement.

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Advisory Board Member

Absolute® May 2023 - Present

New Delhi, Delhi, In

As an Advisory Board member at Absolute, I provide strategic guidance and expert insights to help the organization achieve its goals by providingStrategic Guidance: Offer expert advice on business strategy, market positioning, and growth plans to help the organization navigate challenges and seize opportunities.Industry Insights: Provide valuable insights and trends from your extensive industry experience, helping the organization stay ahead of the curve.Risk Management: Identify potential risks and advise on mitigation strategies to ensure the organization’s long-term sustainability.Networking: Leverage your professional network to connect the organization with potential partners, investors, and industry experts.Innovation Support: Encourage and support innovative ideas and initiatives, fostering a culture of creativity and continuous improvement.Mentorship: Serve as a mentor to the leadership team, offering guidance and support to help them make informed decisions.Feedback and Evaluation: Participate in board meetings, providing constructive feedback on key decisions and initiatives.Advocacy: Advocate for the organization’s mission and vision, helping to build its reputation and credibility within the industry.

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Mentor

Climate First Mar 2023 - Present

London, England, Gb

Climate First is a climate tech accelerator that supports the most ambitious founders solvingthe world’s most pressing problems. As a Climate First mentor, I support a cohort of tenhighly promising companies that are developing sustainable and scalable products that willaccelerate the world’s transition to net zero emissions.As a mentor at Climate First, I provide guidance and support startups that are focused on climate solutions and sustainable innovations viaStrategic Advisory: Offer strategic advice on business models, market entry, and growth strategies to help startups effectively address climate challenges.Technical Guidance: Provide expert knowledge on sustainable technologies and practices, ensuring startups develop viable and impactful solutions.Networking and Connections: Leverage your network to connect startups with potential investors, partners, and industry leaders, facilitating collaborations and funding opportunities.Workshops and Training: Conduct workshops and training sessions on critical topics such as climate impact assessment, sustainable development, and regulatory compliance.Feedback and Evaluation: Participate in pitch sessions, offering detailed and constructive feedback to refine business plans, prototypes, and presentations.Resource Access: Help startups access essential resources, including funding, technology, and market insights, to accelerate their growth and impact.Continuous Support: Provide ongoing mentorship and support, offering advice and encouragement to help founders navigate challenges and achieve their goals.Innovative Thinking: Encourage innovative thinking and problem-solving, inspiring startups to explore new ideas and approaches in the climate tech sector.Impact Assessment: Assist startups in measuring and communicating the environmental and social impact of their innovations, emphasizing the importance of sustainability and community engagement.

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Mentor

Women In Food And Agriculture Mar 2023 - Present

London, Gb

As a mentor for Women in Food and Agriculture, I do the belowEmpower and Advocate: Promote gender equality and support women in the industry.Strategic Advice: Guide business development and market strategies.Technical Expertise: Share knowledge on agricultural practices and innovations.Networking: Connect women with investors and industry leaders.Workshops: Conduct training on leadership and business skills.Feedback: Provide constructive input on pitches and plans.Support: Offer ongoing mentorship and emotional encouragement.

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Scientific Advisor

Viridian Seeds May 2022 - Present

Cambridge , Gb

Develop super-legumes that are climate resilient and ecofriendly Our mission is to create super-legumes to provide sustainable source of healthy, nutritious plant proteins. We plan to do this by creating new super-legumes using tilling and gene editing that will reduce carbon emission by more than 50%. Our products are non-GMO and consumer friendly.Fund Raising: Investor Scouting: Identify and research potential venture capitalists and investors who have a strategic interest in agritech and sustainable innovations.Networking: Leverage your professional network to connect Viridian Seeds with high-potential VCs and investors.Pitch Development: Assist in crafting compelling pitches and presentations Investor Relations: Facilitate introductions and meetings between Viridian Seeds and prospective investors, fostering strong relationships and building investor confidence.Due Diligence Support: Provide guidance on preparing comprehensive due diligence materials to meet investor requirements.Research and Development Guidance: Provide expert advice on developing innovative seed technologies and improving crop yields through cutting-edge research.Technical Expertise: Offer insights on the latest advancements in seed biology, genetics, and biotechnology to guide product development.Training and Mentorship: Educate and mentor the research and development team on best practices and emerging trends in the field.Innovation Advocacy: Promote a culture of innovation within the organization by encouraging the exploration of new ideas and technologies.Data Analysis: Analyze research data to identify trends, draw conclusions, and make recommendations for future projects.Industry Engagement: Represent Viridian Seeds at conferences, workshops, and industry events to share knowledge and build networks.

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Agrobioscience Chief Scientist

Um6P Ventures May 2022 - Present

Ben Guérir, Ma

As an Agribioscience Chief Scientist and consultant in my current consultant work for a VC firm, I performed the below listed activities and my focus was to help global startups realize the potential and help convert ideation into startup ventures. Essentially a startup hatcher and nurser. Evaluation and analysis of technical and subject area domains under agrobioscience vertical that is related to agriculture challenges in Morocco and greater Africa. Please see the below infographic for my VC activities in the agtech/agrifoodtech vertical and venture builder support services for startups.• Agtech/agrifootech/synbio/healthtech/lifesciences investment thesis• Investment strategy• Deal flow• Agtech investment domain market trends & drivers• Venture scout• Technology scouting• Startup scouting• Sourcing global agtech/agrifoodtech startups• Due-diligence• Investment memo/Investment team contribution• Helping startups at 360o• Venture builder support to startups• Technology licensing• Scaling• Connecting startups to scaling partners• Building startups• Mentor• Coaching startups• Panelist• White paper and blogs• Startup use case study• VC outreach• VC branding• Editor• Scientific Journal & Grant Reviewer• Judge in Agri-FoodTech Challenge Awards• Science Communications• OCM Member• Moderator• Scientific & Technical Due-Diligence• Global Networking• Regulatory• Market & Competitive Research• Science Pitch Deck• Licensing IP & Technology• Product Development |• Technology Sourcing & Commercialization

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Agri-Foodtech Sme For Um6P Ventures

Um6P Ventures Nov 2021 - May 2022

Ben Guérir, Ma

Science Advisor/Agri-FoodTech Subject Matter Expert For UM6P Agtech Ventureso Agtech/Agri-foodtech SME for UM6P ventures based in Moroccoo Identification and recommendation of agricultural problems and challenges relevant to African continento Scouting for agtech companies/startups working on innovations that can provide solutions to African small holder farmers agricultural issues and problemso Introductory call with potential agtech industry partners and introduction to UM6P venture teamo Scientific due-diligence analysis and written report on agtech clients to UM6P investment committeeo Recommendation on go/no-go decision on pre-seed/seed investment in agri-food tech companies

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Advisory Board Panel Member For The World Bioprotection Forum (Wbf)

The World Bioprotection Forum (Wbf) Jan 2022 - Present

The WBF is a non-profit organisation that encourages collaboration between the biocontrol industry and academia in the AgriTech sector. Its goal is to connect stakeholders, enabling them to work together to ensure the successful development and commercialisation of biocontrol products.The WBF brings together innovators, industry and academia through membership communications and specialist events featuring presentations from affiliated experts and stakeholders.It is also spearheading an industry-wide lobbying campaign for regulatory reform for bioprotection products.Encourage collaborations between academia and industry for research and commercialisationAdvance the development of next-generation formulation and delivery systems to improve efficacySupport innovators and entrepreneurs in their efforts to raise investment for product development and commercialisationEncourage different stakeholders to work together to address challenges and advance the biocontrol industry
Sab (Scientific Advisory Board) Member

Myoworks Jul 2021 - Present

Generating Scaffolds For Cultivated Meat Industry. As a Scientific Advisory Board Member for MyoWorks in India, I am involved in providing strategic guidance and scientific expertise to help the company innovate and grow. Here’s a detailed description of my contributions:Scientific Expertise: Offer cutting-edge knowledge and insights in biotechnology and related fields to guide MyoWorks’ research and development efforts.Strategic Advisory: Provide strategic advice on product development, market positioning, and long-term growth plans.Innovation Leadership: Promote a culture of innovation, encouraging the exploration of new technologies and methodologies in product development.Quality Assurance: Ensure that MyoWorks’ products meet high standards of quality, safety, and regulatory compliance.Collaborative Projects: Facilitate collaborations with academic institutions, research organizations, and industry partners to drive innovation and access new resources.Mentorship: Mentor the research and development team, sharing best practices and emerging trends in biotechnology and bioengineering.Networking and Outreach: Represent MyoWorks at industry conferences, workshops, and events to build networks and enhance the company’s visibility.Fundraising Support: Assist in identifying funding opportunities and engaging with potential investors to secure financial support for research initiatives.

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Judge

Https://Www.Thesoychallengelive.Com/ Dec 2022 - Present

As a judge in the Soybean Innovation Challenge, I am involved in evaluating innovative ideas and projects focused on soybean cultivation, processing, and utilization. Here’s a detailed description of my contributions:Evaluation: Assess innovative proposals and projects based on criteria such as feasibility, innovation, impact, and sustainability.Feedback: Provide detailed and constructive feedback to participants, helping them refine their ideas and improve their presentations.Scoring: Score entries objectively and fairly, considering all aspects of the projects, from scientific merit to market potential.Mentorship: Offer mentorship and guidance to participants, sharing your expertise and insights to help them develop their innovations.Panel Discussions: Participate in panel discussions to deliberate on the merits of different projects and select the winners.Trend Analysis: Stay informed about the latest trends and advancements in soybean research and technology to ensure informed evaluations.Networking: Facilitate connections between participants and industry leaders, fostering collaboration and innovation.Promotion: Promote the challenge and its objectives, helping to raise awareness and attract high-quality entries.
Technical Judge

Uae Foodtech Challenge Jul 2022 - Jun 2023

Abu Dhabi, Abu Dhabi, Ae

As a Technical Judge in the UAE FoodTech Challenge, I am involved in critically evaluating innovative food technology solutions. Here’s a detailed description of my contributions:Evaluation: Assess technical proposals and projects based on criteria such as innovation, feasibility, scalability, and impact on the food industry.Scoring: Provide objective and detailed scores for each entry, ensuring a fair and balanced assessment process.Feedback: Offer constructive feedback to participants, helping them improve their solutions and presentations.Expert Guidance: Share your expertise in food technology to guide and support the development of cutting-edge solutions.Panel Deliberations: Participate in panel discussions to debate and select the most promising projects.Trend Analysis: Stay informed about the latest advancements in food technology to ensure that evaluations are current and relevant.Mentorship: Provide mentorship to participants, helping them navigate technical challenges and refine their innovations.Networking: Facilitate connections between participants and key industry stakeholders to foster collaboration and growth.

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Plant Biology/Plant Pathology Lead

Greenlight Biosciences, Inc. May 2018 - May 2021

Medford, Ma, Us

Development of exogenous RNA based solutions for crop protection• Literature/patent survey, identification of RNAi gene/mRNA targets, design of dsRNA for single target and hybrid dsRNA for multiple targets• Lead for method development of dsRNA delivery into crop plants • Demonstrated delivery of dsRNA into 7 plants for the first time in the company• Showed phenotypic and molecular evidence to demonstrate successful delivery of dsRNA trigger in plants• Developed and introduced stem-loop RT-PCR to the company to detect the 21 mer siRNA diced from input dsRNA• For the very first time in the company, showed siRNA processed from dsRNA trigger delivered into plants using SLRT-PCR• Showed systemic movement of dsRNA trigger and siRNA in whole plants• Using qRT-PCR, dsRNA mediated silencing of target gene expression in planta was shown for the first time in company• For the very first time, showed phenotype development in plants upon silencing of target marker genes via dsRNA trigger• Identified key targets for pathogen protection via exogenous dsRNA triggered RNAi• Developed TRV based VIGS silencing • Introduced to the company, phenotype plant markers for silencing other than PDS, such as PB7, RPS6, Chl H and Chl I, • Provided preliminary evidence for development of disease resistance trait in crops by dsRNA induced exogenous RNAi

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Agbiotech Advisor/Consultant For Plant Molecular Farming

Moolec Science Aug 2020 - Dec 2020

Luxembourg, Lu

Genetic engineering based molecular farming to express animal proteins in plants • Develop a strategy and science plan for plant molecular farming to express GOI/POI in plants• Ideation and method development for design, and construction of Agrobacterium based plant transformation vectors• Advise on use of best combination of regulatory and enhancer elements to achieve maximum POI expression in transgenic events• Develop a screening strategy based on Agrobacterium-mediated transient transformation to determine efficacy of vectors• Advise on selection and manipulation of Agrobacterium strains for enhanced transformation efficiency• Strategy to develop marker-free plants• Cloning strategy for vector development, molecular assays to screen and characterize transformants

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Principal Scientist, R&D

Edison Agrosciences, Inc. Jan 2016 - Apr 2018

St. Louis, Mo, Us

Enhancing plant genetic transformation by Agrobacterium. Development of an efficient transformation system for sunflower. Developing and commercializing innovative solutions for the production of plant-based industrial materials, with a primary focus on the development of alternative rubber crops (sunflower) Lead for sunflower transformation/tissue culture and molecular biology Set up tissue culture/plant transformation and molecular biology lab independently for Edison Agrosciences, Inc. Isolated, cloned and developed binary plant transformation constructs harboring rubber pathway target genes for sunflower and model plant transformation Generated >70 binary gene constructs, >70 Agrobacterium strains and >80 E. coli strains independently (just myself) Developed an efficient adventitious shoot bud based and direct regeneration system for sunflower Designed and cloned engineered key rubber pathway target gene fusions in binary plasmids and developed Agrobacterium strains for model plant transformation Generated and did molecular analysis on hundreds of transgenic events in model plants expressing key rubber pathway target genes for evaluation of rubber synthesis Preliminary data on rubber quantitative analysis revealed transgenic plants with a particular rubber target gene fusion showed rubber production in the model plant event Conceived, developed and established viral delivery mediated transient expression system for sunflower and model plant Generated multigene (stack of 5 genes) binary constructs for viral mediated transient expression Discovered 2 rubber target genes to confer significant increase in rubber production in sunflower upon viral mediated transient expression Co-technical lead and subject matter expertise in NSF funded STTR project on “Enhancing Agrobacterium-mediated plant transformation efficiency and quality of transgenic events using plant genes and chromosomal T-DNA launch”Training co-scientists on tomato transformation

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Scientific Consultant

Edison Agrosciences, Inc. Feb 2015 - Dec 2015

St. Louis, Mo, Us

Project: Developing and commercializing innovative solutions for the production of plant-based industrial materials, with a primary focus on the development of alternative rubber crops. • Consulting on a NSF funded STTR Phase I project “Enhancing Plant Genetic Transformation by Agrobacterium” to increase the transformation efficiency and quality of transgenic events in recalcitrant crops by using transformation enhancing plant genes and launching T-DNA from Agrobacterium chromosome. Technology will be applied to express rubber production in sunflower. • Scientific assistance for a project aimed at “Developing a sunflower rubber crop to diversify global rubber production and provide an alternate rubber source for USA”• Responsible for genetic engineering and molecular biology lab set up, identification of vendors for molecular biology equipment and supplies for genomic DNA, total RNA isolation and analysis by end-point PCR and qPCR.• Method validation and development of SOPs (Standard Operating Procedure) for nucleic isolation, PCR, qPCR, Southern & Northern blotting for sunflower and Arabidopsis• Development of an Agrobacterium-mediated transformation method for sunflower• Molecular characterization of several transgenic tomato and Arabidopsis lines to test the effect of novel Agrobacterium strains on the frequency of generation of single-copy events without extraneous DNA sequences (vector backbone) flanking the T-DNA• Selected as the principal investigator (PI) for NSF-STTR Phase-I grant proposal on “Targeted Agrobacterium T-DNA integration for predictable and stable plant transgene expression”• Developing and validating SOPs (Standard Operating Procedure) for sunflower transient and stable transformation, virus (TRV)-mediated transient gene expression for sunflower & model plant, nucleic acid isolation, cloning, PCR, qPCR, qRT-PCR, Southern blotting, • Training co-scientists in CO-PI lab (Stanton Gelvin, Purdue University) on tomato transformation

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Senior Research Scientist

Cibus Global Aug 2012 - Jul 2014

San Diego, California, Us

Project: Development of gene-edited potato resistant/tolerant to a devastating fungal disease and to test the effect of allelic variation of target genes on plant defense hormone accumulation and disease resistance to bacteria in Arabidopsis•Identification of disease resistance/tolerance gene target leads for genome editing and validation in model plants•Develop and devise strategy for genome editing via RTDS (Rapid Trait Development System) using GRON (Gene Repair Oligo Nucleotide) directed DSB (Double-Stranded Break) repair• Constructed Agrobacterium binary plasmid vectors and completed molecular characterization of over 200 transgenic potato lines containing variants of the 3 target genes. Determined gene copy number and expression by qPCR and qRT-PCR, respectively • Performed detached leaf infection assay for a fungal pathogen and identified disease-tolerant potato lines • Identified by TAIL-PCR the T-DNA integration site in the disease-tolerant potato transgenic event • Developed a highly efficient Agrobacterium-floral dip transformation system for Arabidopsis T-DNA insertion mutant for plant defense and development-related hormone to transfer target alleles containing desired modifications • Estimated transgene copy number and gene expression in over 200 complemented T1 Arabidopsis lines by qPCR and qRT-PCR. Identified low copy transgenic lines (1-2 T-DNA copies) with high level of gene expression for functional studies• Determined zygosity by qPCR in 140 T2 progeny of 14 T1 lines (10 progeny per line) and identified homozygous lines. • Developed leaf infiltration based bacterial infection assay for Arabidopsis-Erwinia host-pathosystem• Identified transgenic lines resistant to bacterial infection and necrotic disease• Demonstrated the SNP (single amino acid change) in target gene conferred high level of tolerance to pathogen infection in both crop and model plants and dramatic reduction in disease symptoms• Developed drought assay

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Agrobacterium Researcher (Post-Doctoral Associate)

Samuel Roberts Noble Foundation, Usa Jun 2010 - Jul 2012

Ardmore, Ok, Us

Project: Identification of plant genes involved in Agrobacterium-mediated genetic transformation using Arabidopsis and Nicotiana benthamiana as models. The specific objective is to unravel the role of host DNA-repair machinery proteins in Agrobacterium-mediated plant transformation. Identification of plant genes involved in Agrobacterium-mediated plant transformation will help us to increase the transformation efficiency of transformation recalcitrant crops like Soybean and Maize. • In a collaborative project with Prof. Stanton B Gelvin (Purdue University, Indiana), I Identified Arabidopsis Non-Homologous End-Joining (NHEJ) gene mutants Ku70, Ku80, Artemis and DNA Ligase 6 to increase the Agrobacterium-mediated stable transformation efficiency in Arabidopsis root segments using tumor assay.• NHEJ genes Ku70, Ku80, Artemis and DNA Ligase 6 also found to increase stable transformation efficiency in N. benthamiana upon down-regulation by VIGS-mediated gene silencing• As a co-investigator, showed that Agrobacterium targets NHEJ protein XRCC4 and delays NHEJ-mediated double strand DNA break repair to favor T-DNA integration and increase transformation • Demonstrated Bacterial type III effector to increase Agrobacterium-mediated stable transformation Publications:1. Agrobacterium may delay plant non-homologous end joining double strand DNA repair via XRCC4 to favor T-DNA integrationZarir Vaghchhipawala, Balaji Vasudevan, Seonghee Lee, Mustafa Morsy and Kirankumar S MysorePublished in PLANT CELL , Oct 20122. "Agrobacterium T-DNA integration into the plant genome can occur without the activity of key non-homologous end-joining proteins"So-Yon Park , Zarir Vaghchhipawala , Balaji Vasudevan , Wanda Waterworth , Christopher West , Stanton Gelvin (corr-auth) , Kirankumar MysorePublished in PLANT JOURNAL , Feb 2015SYP, ZV and BV equally contributed to this work

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Post Doctoral Associate

Cornell University, Nysaes, Usa May 2008 - May 2010

Ithaca, Ny, Us

Project: Generation of transgenic tomato resistant to wilt and canker disease caused by a Gram-positive bacterium Clavibacter michiganensis subsp. michiganensis (Cmm) • Identified 6/160 genes in a VIGS screen on N. benthamiana to play a role in host basal defense to wilt and canker disease caused by Cmm. • Cloned and over-expressed two potential candidate genes snakin-2 and extensin-like protein (ELP) in transgenic tomato plants that conferred tolerance to Cmm disease • Published the findings in peer-reviewed international journals (Transgenic Research and Phytopathology). • In a collaborative project with Prof. Tomas Burr laboratory at Cornell University, over-expressed truncated VirE2 of Agrobacterium tumefaciens in transgenic grape vine and showed resistance to an economically important crown gall disease. Results published in the premier journal Transgenic Research (2010) • Supervised graduate students (MS and Ph.D)Publications:1) Manuscript entitled ““Over-expression of snakin-2 and extensin-like protein genes restricts pathogen invasiveness and enhances tolerance to Clavibacter michiganensis subsp. michiganensis in transgenic tomato (Solanum lycopersicum)” Transgenic Research (2011), Published OnlineVasudevan Balaji and Christine D Smart2) Silencing of Host Basal Defense Response-Related Gene Expression Increases Susceptibility of Nicotiana benthamiana to Clavibacter michiganensis subsp. michiganensis (2011). Phytopathology, Vol 101:349-357. (http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-05-10-0132)Vasudevan Balaji, Guido Sessa and Christine D SmartEditor's pick for the month of March 2011(3) Resistance to crown gall disease in transgenic grapevine rootstocks containing truncated virE2 of Agrobacterium (2010). Transgenic Research, 19: 949-958 (http://www.springerlink.com/content/l38213445p436p16/fulltext.pdf)Stoyanka V. Krastanova, Vasudevan Balaji, Michele R. Holden, Mary Sekiya, Baodi Xue, Esengul A. Momol, and Thomas J. Burr

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Senior Scientist

Metahelix Life Sciences Pvt. Ltd., India Mar 2007 - Mar 2008

Projects: To develop transgenic tomato, cotton and okra resistant to Tomato Leaf Curl Virus (ToLCV), Cotton Leaf Curl Virus (CLCuV) and Okra Yellow Vein Mosaic Virus (OYVMV), respectively using RNA interference (RNAi)• Scientific manager for 3 projects and supervised scientists and research assocaites• Devised scientific strategy, formulated elaborate time and science plan, managed all the 3 projects and achieved set targets • Successfully cloned and sequenced all the proposed 5 viral targets for RNAi from virus-infected tomato, cotton and okra leaf samples exhibiting typical disease symptoms• Developed 15 Agrobacterium tumefaciens strains (LBA4404, EHA105 and GV3101) containing RNAi plasmid pKANNIBAL with viral targets in sense and anti-sense • Generated 60 clones in 7 months with the assistance of only one working hand. Completed the gene and vector development part of the project well in advance of the set time schedule and transferred all the Agro-binary constructs to tissue culture division for the generation of transgenic plants
Post-Doctoral Fellow

Tel Aviv University, Israel Nov 2003 - Feb 2007

Tel Aviv, Il

Projects: Transcriptome analysis and identification of genes involved in resistance response of tomato to leaf spot disease caused by Xanthomonas campestris pv. Vesicatoria (Xcv). Molecular dissection of wilt and canker bacterium Clavibacter michiganensis subsp. michiganensis (Cmm) interaction with tomato• Identified by microarray analysis, novel genes involved in tomato resistance response to Xcv• Unveiled the transcriptome changes in tomato during Cmm attack and wilt disease progress• Identified ethylene as a key signal regulating susceptible response of tomato to Cmm• First researcher to use Affymetrix tomato chip for any kind of microarray analysis previously reported• Published 4 articles in peer reviewed international journals of repute with high impact factor and citations (Plant Physiology, MPMI, FIG, PSB)• Deposited microarray data in Tel Aviv University link and Tomato Expression Database (TED) of Cornell University, USA• Standardized Virus-Induced Gene Silencing (VIGS) system in tomato and Nicotiana benthamiana• Supervised graduate students (master and Ph.D)Publications:1) (2008). Plant Signaling and Behaviour, 3: 839-84, (http://www.landesbioscience.com/journals/psb/BalajiPSB3-10.pdf) Vasudevan Balaji and Guido Sessa2) (2008). Plant Physiology, 146: 1797-1809(http://www.plantphysiol.org/cgi/reprint/146/4/1797)Vasudevan Balaji, Ofra Sherf, Maya Mayrose, Jasmine Jacob-Hirsh, Rudolf Eichenlaub, Naim Iraki, Shulamit Manulis-Sasson, Gideon Rechavi, Isaac Barash and Guido Sessa3) (2007). Functional and Integrative Genomics, 7: 305-316, (http://www.springerlink.com/content/fjn2703256507742/)Vasudevan Balaji, Avi Gibly, Paul Debbie and Guido Sessa4) (2004). Molecular Plant-Microbe Interactions, 17: 1212-1222, (http://apsjournals.apsnet.org/doi/pdf/10.1094/MPMI.2004.17.11.1212)Avi Gibly, Arale Bonshtien, Vasudevan Balaji, Paul Debbie, Gregory B. Martin and Guido Sessa

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Ph.D Graduate Student

Madurai Kamaraj University Jul 1996 - May 2003

Madurai, Tamil Nadu, In

• Ph.D thesis title: Development of a transformation system for Vigna mungo and analysis of the DNA B clone (KA27) of Mungbean yellow mosaic virus-Vigna.• Demonstrated the application of extra copies of virG gene in increasing Agrobacterium-mediated transformation efficiency for a highly recalcitrant pulse crop urd bean alias black gram (Vigna mungo).• Successfully cloned and manually sequenced one DNA B component of the bipartite geminivirus Mungbean Yellow Mosaic Virus-Vigna (MYMV-Vig), causative agent of yellow mosaic disease in black gram.• Demonstrated the use of replicase gene of MYMV-Vig to confer resistance to virus in transgenic tobacco.Publications:1) Expression of full-length and truncated Rep genes from Mungbean yellow mosaic virus-Vigna inhibits viral replication in transgenic tobacco (2006). Virus Genes, 33: 365-374P. V. Shivaprasad, P. Thillaichidambaram, V. Balaji and K. Veluthambi2) Infectivity analysis of two variable DNA B components of Mungbean yellow mosaic virus-Vigna (MYMV-Vig) in Vigna mungo and Vigna radiata (2004). Journal of Biosciences, 29: 297-308V. Balaji, R. Vanitharani, A. S. Karthikeyan and K. Veluthambi3) Analysis of an isolate of Mungbean yellow mosaic virus (MYMV) with a highly variable DNA B component (2004). Archives of virology, 149: 1643-1652A. S. Karthikeyan, R. Vanitharani, V. Balaji, S. Anuradha, P. Thillaichidambaram, P. V. Shivaprasad, C. Parameswari, V. Balamani, M. Saminathan and K. Veluthambi4) Agrobacterium-mediated transformation efficiency in blackgram and rice enhanced by multiple copies of pTiBo542 virB and VirG (2003). Indian Journal of Biotechnology, 2: 138-146V. Balaji, P. Rajamuni, G. Sridevi and K. Veluthambi5) Efficient vir gene induction in Agrobacterium tumefaciens requires virA, virG and vir box from the same Ti plasmid (2001). Journal of Bacteriology, 183: 4079-4089A. Krishnamohan, V. Balaji and K. Veluthambi

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Balaji Vasudevan, Ph.D. Skills

Molecular Biology Pcr Dna Life Sciences Genomics Qpcr Rt Pcr Bioinformatics Tissue Culture Protein Chemistry Genetics Plant Transformation Sequence Analysis Cell Western Blotting Elisa Agrobacterium Biology Transgenic Event Selection Genetic Engineering Molecular Scientific Writing Real Time Polymerase Chain Reaction Adventitious Dna Testing Southern Blot Tail Pcr To Identify T Dna/transgene Integration Site In Gm Plants Molecular Plant Pathology Cloning By Conventional And Gateway Gus Histochemical And Mug Assay Assay Development For Molecular Plant Microbe Interaction Research Trait Discovery Zygosity Analysis Transgenic Event Characterization Protein Over Expression Protein Purification Using Affinity Tags Northern Blot Qrt Pcr Yeast Two Hybrid Bifc Virus Induced Gene Silencing Pathogen Bio Assays Marker Free Plants Site Directed Mutagenesis Microarray Analysis Suppression Subtractive Hybridization Scientific Presentation Develop And Manage Scientific Research Projects Training And Development Of Skilled Researchers

Balaji Vasudevan, Ph.D. Education Details

Madurai Kamaraj University

Plant Molecular Biology And Biotechnology
University Of Madras

Plant Science
University Of Madras

Botany

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Balaji Vasudevan, Ph.D. attended Madurai Kamaraj University, University Of Madras, University Of Madras.

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Balaji Vasudevan, Ph.D. has skills like Molecular Biology, Pcr, Dna, Life Sciences, Genomics, Qpcr, Rt Pcr, Bioinformatics, Tissue Culture, Protein Chemistry, Genetics, Plant Transformation.

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Balaji Vasudevan, Ph.D.'s colleagues are Catherine Fulton, Jan Mccormick.

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