Mithu Banerjee Email & Phone Number

Franklin Lakes, New Jersey, Us

• Performed staining of OneFlow tubes, installed assays, ran OneFlow tubes, and analyzed data in FACSuiteClinical, resulting in timely launch of OneFlow tubes with universal loader• Served as the validation lead for the OneFlow IVDD with universal loader in FACSlyrics• Prepared experimental plan to design different workflows and testing strategy to meet the DDS and Risk Analysis requirements and drafted protocol, guideline. Updated user-facing documents• Presented the design review and helped in writing the final validation report after reporting all findings in E log and storing all results within proper folder in Microsoft teams• Contributed in the validation of the new FACSuiteClinical 1.5 and FACSuite 1.5 Software• Proficient in creating and sending orders through FACSlink, and retrieving results through FACSlink

• Performed primary cell culture by isolating PBMC cells from whole blood (healthy and CFS patients) using different methods: viability check after setting up the cell culture lab• Planned protocols, panel design for the FACS machine, ran several samples, studied immune profile analyzed data and maintained the FACS machine • Separated T cells, PBMC cells using the fully automated Cell separator RoboSep• Designed the cytokine panel to study Lime disease, extensively used Magpix instrument to run clinical samples and analyzed the data to generate reports. Conversant with handling samples in the Luminex machine. • Isolated DNA/RNA from clinical specimens, followed by PCR amplification and purification and performed clinical tests of samples in Randox Investigator, conversant with nanodrop and quibit • Hands on experience in working in-vivo with mouse models to understand the mechanism of action of potential cancer drugs • Secluded RNA from control samples using well known reagent kits followed by running samples in the QTPCR machine with a proper positive control, completed data analysis • Corona Virus Testing - Planned the experiment and ran samples in Randox Investigator starting from viral RNA isolation, Multiplex PCR amplification, biochip hybridization, sample preparation for Covid-19 detection using real-time qPCR. • Hands on knowledge about single cell RNA sequencing using the inDrop fluidics platform: Isolated PBMC cells, counted cells using Countess II, made cell suspension, set up the InDrop Instrument, library preparation, quantification using Nanodrop, Bioanalyzer and Qubit, PCR Amplification, setting up for sequencing.

Santa Clara, California, Us

• Performed Bacterial cell culture, isolated plasmid using miniprep, transformed bacterial cells for the bioluminescence project• Ran agarose gel electrophoresis after performing restriction digest and PCR amplification to test the quality of the plasmid• Accomplished genetic engineering by designing primers for the Lux Operon using the Snap gene•Performed ligation of pBAD and Lux Operon to insert Lux Operon gene into the pBAD Plasmid backbone to generate persistent bioluminescence• Isolated DNA/RNA , amplified (PCR1), PCR1 purification, tested DNA/RNA quality control using Bioanalyzer, quantified using Nanodrop, PCR2 and purification. Final quantification using Qubit (Fluorometer). •Completed pooling libraries, library denaturation and thorough knowledge about loading samples in the illumina sequencing machine

Santa Clara, Ca, Us

• Solved, explained and analyzed problems from chemistry and biochemistry books• Provided step by-step textbook solutions and guidance to college students.

Chicago, Il, Us

• Teach chemistry and biochemistry to high school and undergrad students.• Help students to design and develop laboratory skills and experiments

Mumbai, Maharashtra, In

Expertise:• Cancer, normal and neuronal Cell Culture, several cell based assays• Plasmid Isolation, DNA gel• Stable and transient transfection and establishing stable cell line • Immunostaining, Apoptosis Assay using Annexin V. Microscopic imaging. FACS. • Microtubule dynamics study using Confocal Laser Scanning Microscope. • Protein Isolation and Purification. Western blot.• Biophysical study using Fluorimeter and Circular Dichroism Spectrophotometer. • Electron Microscopy• Ligand docking with GOLD and Ligand Fit in Discovery Studio.

In

Expertise:• Protein isolation, purification and conformational study• Chaperone and protein folding study • Biophysical study using Fluorimeter, Spectrophotometer, Stopped Flow and Isothermal Titration Calorimeter.• Homology Modeling • Docking of drug on receptor using GOLD and DOCK in insight II computer graphics

Ph.D From Bose Institute, Biochemistry

M.Sc, Chemistry

B.Sc In Chemistry