Barbara Paes Email & Phone Number

Montreal, Quebec, Canada

Montreal, Quebec, Canada

Support all aspects of research associated with the Viral Vectors and Vaccines Bioprocessing Lab.- Designing and executing production experiments with mammalian cell lines; including production in bioreactors, purification (downstream processing).- Implementing analytical protocols for recombinant proteins and viral vectors.- Writing protocols and reporting the data for technical reports to clients.- Organizing and analyzing the data for contribution to scientific publications.- Managing lab inventory, purchases, equipment installation and maintenance, ensuring all Environmental Health and Safety (EHS) regulations are implemented.

Ribeirão Preto E Região, Brasil

• Investigated protocols for hematopoietic differentiation of iPS cells.• Assisted in establishing and maintaining an biorepository of iPS cells from sickle cell anemia patients.• Carried out the culture of peripheral blood mononuclear cells and human pluripotent stem cells, and a variety of assays.• Evaluated the expression of pluripotency and hematopoietic markers along the differentiation through immunofluorescence analysis via flow cytometry.• Collaborated with peers on academic research projects and published scientific articles.• Assisted with laboratory and equipment cleaning and maintenance, material sterilization, organization and preparation of reagents and supplies.• Co-organized and supervised an annual summer course at the university level; planned and taught workshops on Pluripotent Stem Cells and Hematopoietic Differentiation for undergraduate students.

Toronto E Região, Canadá

• Summer Intern at The Shoichet Lab, Institute of Biomaterials and Biomedical Engineering.• Carried out the culture and differentiation of H9 human embryonic stem cell line into retinal progenitor cells.• Evaluated photoreceptor markers to characterize hES derived retinal progenitors cells along differentiation through RT-qPCR.• Performed immunostaining and imaging acquisition for analysis of retinal lineage specific molecular marker using confocal microscope.

Toronto E Região, Canadá

Internship at TCP Transgenic Core on Manipulating Mouse Embryonic Stem (ES) Cells. Hands-on experience included mouse embryonic stem cell culture, thawing, freezing, and expansion; harvesting of oocytes; preparation of cells for aggregation and microinjection.

Scholarship - Undergraduate Research

Assis E Região, Brasil

• Intern at Laboratory of Plant Biotechnology, Department of Biological Sciences.• Molecular characterization of the limonoid udp-glucosyltransferase gene from commercial varieties of sweet orange and tangerine.• Conducted molecular biology analysis of plant materials, DNA extraction from citrus plant samples, PCR and agarose gel electrophoresis to study polymorphism.• Collaborated on the development of research projects and presented scientific research posters.

Master’S Degree, Clinical Oncology, Stem Cells And Cell Therapy

Activities and Societies: Dissertation title: Generation of hematopoietic stem/progenitor cells and erythroid progenitor cells.

Bachelor’S Degree, Biotechnology Engineering

Activities and Societies: Laboratory of Plant Biotechnology. Scientific Research title: Molecular characterization of limonoid.

Visiting Student, Biotechnology

Activities and Societies: Scholarship - Science without Borders program, Visiting Undergraduate Student.