Barbara Shields Email & Phone Number

Southampton, Pa, Us

Philadelphia

Creating a syllabus for pre- med chemistry course

Greater Philadelphia Area

The Rodent Breeding Division of Scot-Tech Inc. provides unique consulting services for breeding genetically engineered strains of mice and rats. It provides strategies for managing colonies in ways that protect valuable lines of Tg, Knock Out, Knock In, reduce costs/resources employed, enrich the research outcomes.- Counselled staff at a local University on resolving problems with their breeding colony, which had rendered it incapable of breeding rats to support their research.-Presented a proposal for a "Mouse Center" as a collaboration with a Vendor/Farm to eliminate shipping fees, lower the cost of mice and alleviate the rodents' 3-day period of acclimatization.

Greater Philadelphia Area

As a Certified Tutor in Level I and Advanced Level II, I am currently tutoring students in Biology, Anatomy, Chemistry & Microbiology. I find it rewarding to give students the opportunity to excel in Science..

Greater Philadelphia Area

I was brought on board U of Penn back in 1997. I spent 13 years working independently, producing animal models for breast cancer research, performing advanced transgenic techniques, managed and refined test protocols for breeding mice and rat colonies, directed work of technicians in compliance with animal care, biohazard and radiation safety regulations. KEY ACCOMPLISHMENTS INCLUDE:• Located qualified new source of PMS (NHPP) resulting in increased fertilization yields with concomitant reduced costs and was adopted by other transgenic groups.• Created new breeding program for in-bred strain of mice i.e. “Pregnancy on Demand”. It reduced cost by up to 1/3 of vender’s price and reduced associated space requirements, and, it provided higher accuracy in predicting DOB. The abstract was published in Transgenic Research Magazine October 2008 and was a poster exhibit at ISTT conference, Toronto.• Presented poster at the ISTT Conference in Berlin, on “A Method for Increasing the Production of Transgenic Rats by use of Nutritional Supplements” - see Transgenic Research Magazine, March 2010. • Established informational exchanges with other facilities both in the US and worldwide.• Trained lab colleagues on IP and SC Injection of rodents for transgenic procedures.• Recipient of 2009 Award for Enhancement of Animal Welfare at the Delaware Valley Branch.Research Specialist, Cancer Center (1997 to 2000)Techniques – Nuclear Injection, Embryo Implantation, knock out and knock in transgenic mice, DNA and RNA isolation, PCR, tissue culture, Southern blot analysis, restriction endonucleoses, mice breeding.• Successfully mated r2, 112, sp44, cg27, introduced new protocols for mice breeding, identified, and implemented correlation between age/weight and fertility.For detailed list of accomplishments, please contact me directly.

Philadelphia, Pa

I came on board Temple in 1993 and I held a series of research positions responsible for Nuclear transfer, Transgenic Techniques, ES cells injection, PCR, DNA agarose and polyacrylamide gel electrophoresis, tissue culture, Southern blot analysis, mice breeding, solution preparation. Basic techniques of Molecular Biology, PCR, Gel Agarose, mice breeding, collection, and culture of embryos, nuclear injection, making pipettes for microinjection.• Successfully bred Jak-3 knock out, MMTV Amyb, Alpha 12 and 13 with an 88% implantation rate, both firsts for this lab. • Conducted research on the Interaction between Fibrinogen and Platelets during formation and dissolution of blood clots.• Trained technicians and graduate students in molecular biology techniques.For detailed list of accomplishments, please request my resume.

Warsaw, Poland

Performed research on oxygen dependent mechanisms in adrenal gland and kidneys in the pathogenesis of primary hypertension.• Researched liposomes, tissue culture, and mast cell physiology.• Conducted seminars for students on digestive system, blood coagulation, endocrinology, human reproductive system, histology, biology, and chemistryFor detailed list of accomplishments, please request my resume.