Responsible for production, purification and extensive characterization of a large library of wild type and mutant surfactant protein D proteins from a variety of species. Production was done in RosettaBlue competent cells for recombinant proteins or in sterile mammalian cell culture for full length proteins. Extensive expertise in all aspects of protein purification and characterization including AKTA FPLC, SDS-PAGE, western blotting and Biacore SPR. Successfully contributed to twelve publications in eight years in this position.Previous Experience:UNIVERSITY OF PITTSBURG SCHOOL OF MEDICINEResearch Associate-Department of Molecular Genetics and BiochemistryExperienced in plasmid preparation, restriction enzyme characterization, gel-electrophoresis, Chromium-release cytotoxicity assays, thymidine proliferation assays, gel-filtration chromatography, SDS-PAGE, ELISA, spectrophotometry, animal handling, dissection, tissue culture.CIBA-CORNING DIAGNOSTICS CORPORATIONResearch Associate-Immunochemistry DepartmentPrepared, purified, and characterized protein-conjugate immunogens, acridinium-labeled antibodies, and protein/nucleic acid antigens as well as immobilized antibodies to various solid phases. Subsequently utilized these components to develop ELISA and chemiluminometric screening assays for hybridoma selection. Antibodies commercialized in context of rapid, clinical myocardial infarction testing.Applied small animal and sterile tissue culture techniques to produce monoclonal antibodies including immunizations, sera evaluation, dissection, cell fusions, cloning, microscopy, and ascites production.Extensive experience in antibody purification and characterization including HPLC, affinity chromatography, ion-exchange chromatography, chemiluminometric assays, ELISA, RIA, SDS-PAGE, total protein assays, and spectrophotometry.