• Isolation and identification of Vibrio cholerae strains from clinical samples.• Preparation and Characterization of Vibrio phages.• Establishing host range coverage of vibrio phage cocktail ProphaLytic-Vc™ using isolates from cholera clinical samples• Optimizing the ratios of phages in ProphaLytic-Vc™ to achieve maximum clinicalefficacy

•Isolated and identified Vibrio cholerae strains from clinical samples.•Prepared Vibrio phage Master and Working stocks.•Performed Quality Control of phage liquid preparations: phage purification (lipopolysaccharides extraction and quantification); determination of phage titers; sterility and stability.•Performed Quality Control of different phage formulations (Drug Product): potency and stability testing.•Established host range coverage of vibrio phage cocktail using isolates from cholera clinical samples.•Optimized the ratios of phages in the vibrio phage cocktail to achieve maximum clinical efficacy.•Performed ex-vivo work with tissue samples obtained from animal studies of various identified ratios of phage-cocktail and relevant clinical isolates.•In vitro testing of the protective effects of co-administration of the oral cholera vaccine with vibrio phage cocktail.•Developed and validated sterilization methods of tools and supplies to be used while working with phage and bacteria.•Write SOP of new and established methods for technical transfer.•Collected and analyzed data using a variety of databases (e.g., Benchling, Graph pad PRISM, etc).•Mentored new hires and supervised lab techniques such as Dribble Plaque Assay, Six well Plate Assay, BacterialCulturing and Isolation, Aseptic techniques

• Developed culturing techniques and media to isolate anaerobic bacteria and other diverse bacteria including many previously considered unculturable from human samples. • Supported projects for the development and QC of product candidates to treat diseases.• Responsible for building and maintenance of a large Microbial Library of diverse bacterial strains selected for defined properties.• Performed molecular biology assays and analysis (e.g. DNA extraction, PCR, gel electrophoresis).• Maintained and operated COY and BACTRON Anaerobic Chambers in which aseptic handling, processing, and culturing of anaerobic bacteria from human samples were performed. • Collected and analyzed data using a variety of databases (e.g. FileMaker, Benchling, NCBI, EzBiocloud, LabCloud).

• Analysis of bacterial DNA using Pulsed Field Gel Electrophoresis for use in epidemiological studies.• Performed DNA extractions and PCR in a wide variety of organisms.• Analysis of bacterial DNA using Diversilab system.• Susceptibility testing of Clostridium difficile.• National Susceptibility Survey of Bacteroides fragilis group which includes novel compounds and antibiotic susceptibility testing against Bacteroides fragilis group. • Culture, identification, antimicrobial susceptibility testing of anaerobic and aerobic bacteria.• Clinical Studies involving Probiotics: Lactobacillus rhamnosus GG (LGG) and Bifidobacterium animalis subspecies lactis (BB12)