Carolina Baez-Giangreco Email & Phone Number

Boston, MA, US

• Isolation and identification of Vibrio cholerae strains from clinical samples.
• Preparation and Characterization of Vibrio phages.
• Establishing host range coverage of vibrio phage cocktail ProphaLytic-Vc™ using isolates from cholera clinical samples
• Optimizing the ratios of phages in ProphaLytic-Vc™ to achieve maximum clinicalefficacy

Boston, MA, US

• Isolated and identified Vibrio cholerae strains from clinical samples.
• Prepared Vibrio phage Master and Working stocks.
• Performed Quality Control of phage liquid preparations: phage purification (lipopolysaccharides extraction and quantification); determination of phage titers; sterility and stability.
• Performed Quality Control of different phage formulations (Drug Product): potency and stability testing.
• Established host range coverage of vibrio phage cocktail using isolates from cholera clinical samples.
• Optimized the ratios of phages in the vibrio phage cocktail to achieve maximum clinical efficacy.

Boston, MA, US

• Developed culturing techniques and media to isolate anaerobic bacteria and other diverse bacteria including many previously considered unculturable from human samples.
• Supported projects for the development and QC of product candidates to treat diseases.
• Responsible for building and maintenance of a large Microbial Library of diverse bacterial strains selected for defined properties.
• Performed molecular biology assays and analysis (e.g. DNA extraction, PCR, gel electrophoresis).
• Maintained and operated COY and BACTRON Anaerobic Chambers in which aseptic handling, processing, and culturing of anaerobic bacteria from human samples were performed.
• Collected and analyzed data using a variety of databases (e.g. FileMaker, Benchling, NCBI, EzBiocloud, LabCloud).

Boston, MA, US

• Analysis of bacterial DNA using Pulsed Field Gel Electrophoresis for use in epidemiological studies.
• Performed DNA extractions and PCR in a wide variety of organisms.
• Analysis of bacterial DNA using Diversilab system.
• Susceptibility testing of Clostridium difficile.
• National Susceptibility Survey of Bacteroides fragilis group which includes novel compounds and antibiotic susceptibility testing against Bacteroides fragilis group.
• Culture, identification, antimicrobial susceptibility testing of anaerobic and aerobic bacteria.