R&D DivisionMultiOmics Data Analysis GroupCore Labs - Bioinformatics Hub

As members of a dynamic social network, each cell receives, interprets, and redistributes biological information to define and maintain tissue homeostasis. My dissertation seeks to characterize the factors that perturb cell state and function in disease, injury, and aging, by integrating high-throughput sequencing assays with a combination of experimental techniques, including novel cell lines, animal models, and micro-engineered devices. Pursuing a dual degree in Biomedical Engineering and Scientific Computing. Completed "Microfluidics in Biomedical Sciences" graduate certificate program.Advisor: Carlos Aguilar, PhDTechnical Skills:• Data Analysis: R; Python; Matlab; bash; FIJI/ImageJ • NGS/Bioinformatics: Bulk & single-cell RNA-seq & ATAC-seq; ChIP-seq; CUT&RUN / CUT&TAG; Nanostring; Hi-C; Genome-scale metabolic modeling• Molecular Biology: Cell culture (primary & cell lines); DNA & RNA isolation; RT-PCR/PCR; Immuno-staining (cells & tissues); Lentiviral transductions; Transfections; Flow cytometry; Western blots• Imaging: Confocal and epifluorescence microscopy• Microfluidics: PDMS device design and fabrication (e.g. droplet encapsulation, micro-wells, cell culture & manipulation) Funding: • Rackham Predoctoral Fellowship• NIH T32 Predoctoral Trainee (Microfluidics in Biomedical Sciences Training Grant)• Rackham Travel & Research Grantshttps://scholar.google.com/citations?hl=en&user=n5BJiQYAAAAJhttps://nobel.bme.umich.edu/https://biointerfaces.umich.edu/http://umich.edu/~ufluids/

• Contributed to the MicroC3 (microfluidic cis-coculture) platform, an ex vivo companion diagnostic (CDx) that integrates with clinical workflows to rapidly identify effective treatments for blood cancer patients using a patient's own tumor cells• Wrote specific aims and contributed data to 2 SBIR grants

• Consumables Assay & Reagents Group• Evaluated materials and biochemical assay conditions for an automated library prep platform• Optimized assay formulations for a nucleic acid detection and quantitation module • Communicated concisely in weekly updates and delivered an exit seminar to technical and public audiencesManagers: Rigo Pantoja, Al Eckhardt, Eric Vermaas

• Developed an innovative microfluidic point-of-care diagnostic for the treatment of sepsis• Constructed a COMSOL model to simulate heat transfer and fluid dynamics• Integrated digital droplet PCR, fluorescence imaging, and high resolution melts (HRM) for DNA fingerprinting • Presented at the Summer Research Conference with the support of the UC Scholars Program• US Patent Application PCT/US2017/068313PI: Stephanie Fraley, PhD

• Promoted undergraduate involvement in biomedical research and entrepreneurship• Established research projects in 5 faculty labs for 6 undergraduate student teams across 3 departments• Planned and co-hosted workshop series covering engineering leadership and biomedical entrepreneurship in collaboration with the von Liebig Entrepreneurism Center and the Gordon Engineering Leadership Center• Developed an outreach program to introduce high school students to bioengineering• Facilitated the entry of 4 student teams into the NSF I-Corps Program• Recruited over 30 committee members to assist with project team management and faculty communication• Mentored underclassmen through course selection and research opportunities2015-2016 Co-Chair: Sumit Dan2016-2017 Co-Chair: Shoun Matsuka

• Observed surgical procedures in pediatric orthopedic and plastic surgery departments• Collaborated with mentors to identify problems with engineering solutions in the clinic• Presented solutions to clinical problems as a case study to clinicians and students

• Studied the mechanical-epigenetic behavior of glioblastoma cells in varying mechanical microenvironments and treatments with chemotherapeutic drugs• Performed confocal, fluorescent, and 3D traction force microscopy protocols• Analyzed confocal and FRET data to compute ECM-cell, intracellular, and intercellular force in MATLAB• Prepared polyacrylamide gels of varying stiffness for 3D traction force microscopy• Applied cell culture techniques for passaging and changing media• Presented poster at Faculty Mentor Program (FMP) Research SymposiumPI: Shu Chien, MD, PhDMentor: Sung-Sik Hur, PhD

• Wrote monthly articles covering developments in biomedical research• Made complex research topics accessible to the general public

• Developed a prototype light source in bandage for enhanced wound healing• Explored material and design upgrades to enhance durability, comfort, and manufacturability• Wrote a questionnaire to gauge end-user response to prototype• Assembled new versions of prototype

• Developed a Micro-Manager plug-in to facilitate the acquisition of cardiac videos of Drosophila melanogaster for high-throughput gene screens• Optimized a microscopy protocol to identify candidates for screens of genetic adaptations to hypoxia• Co-authored a methods paper on system for publication in JoVE (Journal of Visualized Experiments)• Partnered in recruitment and training of 3 new interns• Anesthetized flies and performed cardiac screensPI: Rolf Bodmer, PhDMentor: Rachel Zarndt, PhD

• Lab-intensive course that simulated a first-year medical student’s clinical anatomy class• Learned human anatomy and physiology through lectures involving cadavers and prosections• Worked with laparoscopic instruments and robotic surgery simulators

• Studied human anatomy, physiology, and developments in cardiothoracic surgery• Practiced dissection, suturing, tying, cannulation, and valve replacement on porcine hearts• Engineered new, innovative solution for atrial septal defect with two team members