Kv1.1 channel dependent song memorization in Zebra finch. Environmental dependent Kv1.1 differential expression in songbird caudal mesopallium.Lab stock and cleaning management. Help to the students. Lab trainer.Techniques: - Husbandry - AAV-shRNA/Cbh-GFP caudal mesopallium bilateral injection - Patch sequencing - Whole cell patch clamp - Paraformaldehyde intracardiac perfusion - Vibratome/cryostat/compresstome sectioning - Biocytin Dextran Amines (BDA) injection, fluorophore streptavidin conjugation and tracing - Immunohistochemistry - Standard and Airyscan Confocal Imaging - Image amalysis - DNA extraction - PCR - Electrophoresis.R and python basic use. Unsupervised and supervised machine learning with scikit-learn k-mean clustering and time related dataset modeling.

Inferior Olive slice immunohistochemistry on GFP+ sparse injected mouse. Mapping of VGLUT1, VGLUT2 and other inputs in the area. Airyscan confocal imaging of Inferior Olive neurons in different nuclei and sub nuclei.Techniques: paraformaldehyde intracardiac perfusion - brain dissection - vibratome slicing - Immunohistochemistry - tile scan/standard/airyscan confocal imaging - Image analysis - DATA analysis.

High school biology and geology teacher.

Central amygdala neuron and astrocyte patch clamp whole cell: electrical characterisation of oxytocin exposure. Hippocampus extracellular recording.Techniques: mouse handling – parenteral injection – dissection – slicing – patch-clamp whole cell recording – field potential hippocampus recording - DATA analysis.

Neuron culture or hippocampal slice patch clamp whole cell and extracellular recording. Disturbance of metabotropic glutamate receptor mediated long term depression of excitatory synaptic transmission in the rat hippocampus after prenatal immune challenge.Techniques: mouse handling – dissection – slicing – patch-clamp whole cell recording – field potential recording – MAE field potential recording.