Benjamin George Email and Phone Number

Team lead for all MSD/ELISA biomaker assays. Experienced in training new employees and directly supervise junior scientists executing biomarker and in-vitro cell based assays.Team Lead for in-vivo qPCR pipeline. Experienced in executing and training junior scientists in mouse tumor and organ tissue dissemination and processing for pre-IND and IND studies. Plan, develop and execute qPCR experiments for lead asset in IND pipeline. Experience in panel design and data analysis/presentation.Planned and executed Pharmacokinetic (PK) Evaluation of lead clinical asset using Quantitative Electrochemiluminescent Bridging Immunoassays. Utilized PK and ADA assays to support pre-IND and IND pipelines.Experienced in planning and executing multi-color Flow Cytometry panels/assays (Cytec Platform).

Drive biomarker studies of healthy, clinical and cancer donor samples to establish patient selection criteria based on primary asset in Phase I clinical trials.Planned and executed cell proliferation/cytotoxicity assays Drug Binding, Anti-Drug Antibody/Neutralizing Antibody Assays, Bi-specific Antibody (BiTE) Tumor-cell Cytotoxicity Assays, MSD (Single and Multiplex) /ELISA, qPCR, LUMICKS (cell avidity), Immuno-oncology assays and Real-Time Cell Metabolic Analysis (Seahorse) experiments.Experienced in isolating PBMC and T-cells from whole blood/leukopaks and primary T-cell and tumor cell culture.Experienced in planning and executing multi-color Flow Cytometry panels/assays (Cytec Platform). Collected, analyzed, and presented clinical data sets for MSD/ELISA, ADA/PK, qPCR, cytotoxicity live imaging (Incucyte) and LUMICKS (cell avidity) platforms.Experience in viral transduction and expansion of immune-cells for targeted avidity assays. Efficiently performed and documented all experimental procedures in accordance with company standards.Generated validated SOPs for company immunoassays, updated/edited existing SOPs, utilized Electronic Lab Notebooks for sample tracking, experimental data submission and SOPs. Driven team leader in a fast-paced environment, delivering study results on demand while completing daily tasks, and mentoring team of junior scientists.

Characterized secondary sensory brain areas active during whisker stimulation in a mouse stroke model. Experienced in training C57BL/6 mice to perform a yes/no goal directed detection task and utilized combined visual and live behavioral data to asses performance before and after induced cortical ischemic stroke.Performed in-vivo array recordings to identify subcortical brain areas that encode sensory information before and after cortical stroke in trained mice. Utilized in epiflorescence microscopy to confirm induced lesion size and progression in mouse model. In addition, experienced in using machine learning to track mouse whisker movements and facial structure changes.

Characterized hP2X2 receptor mutants associated with age-related and/or environment induced hearing loss. Utilized whole-cell patch-clamp technique on transiently transfected HEK 293 cells in combination with molecular biology to create ion channel mutants/chimeric constructs. Additionally, shadowed physicians and organized community STEM outreach opportunities for high school students.

Investigated the regulation of NMDA receptor unitary properties by intracellular residues on the GluN1 subunits & characterization of human transplanted OPCs in shiverer mouse pups. Learned in-slice whole-cell, inside-out and cell attached patch-clamp techniques and conducted patching experiments on transfected HEK 293 cells and whole-cell experiments on transplanted human oligodendrocyte precursor cells, learned bio-statistical analysis and cell culture.

Provided workstation and some networking support to roughly 1,500 end-users, installed and maintained a wide variety of software and hardware on University and students computers, troubleshooted various end-user problems, provided one-on-one end-user/new hire training in the use of applications and operating systems, generated support documentation for installation and maintenance activities and responded to and maintained end-user tickets in queue.

Investigated the role of Oligodendrocyte Precursor Cell (OPC) glutamate receptors in disease.Conducted in-slice whole-cell patch-clamping experiments on oligodendrocyte precursor cells, bio-statistical analysis, dissections, slicing, fixation and immunostaining of mouse pup brains.