Benjamin Perrin Email & Phone Number

Cambridge, Cambridgeshire, GB

• Designed and wrote protocols for bacterial (at a large scale, up to 24 L) and mammalian (up to 2 L) expression systems in order to adapt protein manufacturing pipelines to a new site as a part of multiple tech.
• Optimized and troubleshot these systems over time as the targets being expressed had little or no prior data, using past experiences and literature review. Rescued 3 proteins so far that did not initially express.
• Owner of tissue culture lab, designed and set up the equipment and materials and workflow in the lab from scratch. Revived, grew, split, and transfected various suspension and adherent cell lines
• Dotted line manager for 2 manufacturing associates, not a direct report but set their daily work and helped them with any issues they had, taught them the systems I designed and any necessary techniques, and acted as a.
• Designed experiments and set work for a summer intern to assist on optimization efforts
• Ran FPLC IMAC purification on AKTA and HPLC characterization. Trained on LCMS and TFF

Cambridge, Cambridgeshire, GB

• In charge of nanobody production for the phage display pipeline. Developed own high-throughput nanobody expression system in E. coli and ran it successfully with 4 fold improvement in expression over standard system in.
• Building expression side of a protein manufacturing pipeline from the ground up, including ordering materials, setting up the lab space, writing protocols, and running bacterial and mammalian protein expression and.
• Ran small (50 mL) scale antibody production in mammalian cells, Octet BLI, and FPLC purification on AKTA to provide samples for final QC for a pipeline developing new rabbit monoclonal antibodies. Coordinated with.
• Manufactured library antibodies at 1 L scale and conducted Western Blot and ELISA QC and data analysis, as well as dealing with logistics and shipping of the product. Coordinated with at least six different teams in UK.
• Regularly interpreted and presented data in team, site, and global meetings
• Bacterial cloning and DNA purification, molecular biology, SDS-PAGE gels. Ran high-throughput PCR cleanup, transformation, and plasmid purification

Farmington, Connecticut, US

• Developed new drug targets for Borrelia burgdorferi
• Techniques used include protein expression and purification, solubility, and crystallography, mouse and Ixodes tick handling, Borrelia transformation and puregening of genomic DNA, immunofluorescence assays, E. coli.

New Haven, CT, US

• Assisted in several malaria research projects looking into new drug targets
• Collected data using giemsa staining of blood smears and parasite counting, handling of mice, PCR, gel electrophoresis, and various other lab techniques including molecular genetic techniques and occasionally assisting.
• Designed and conducted various yeast growth assays, conducted in-depth data analysis on GraphPad Prism

New Orleans, Louisiana, US

• Parasite counting of blood smears, flow cytometry, and other skills similar to those used at Yale
• Handled mice and rats, assisted in upkeep of mosquito colony
• Trained 3 more junior peers on basic molecular biology techniques

Master Of Public Health In Tropical Medicine (Mph/Tm)

Bachelor Of Arts (B.A.)