• Ran day to day operations of an RNA chemistry R&D and production lab• Developed TC RNA synthesis chemistry for robust, CRISPR sgRNA (100+ base) production• Helped plan and start a high-throughput oligonucleotide production program including designing lab space, equipment, and coordinating wit facilities and safety personnel• Trained and supervised activity of Ph.D. scientists• Developed novel plate-based affinity purification method for high throughput production now transferred to production facility• Developed protocols for liquid handling robot to automate production workflow• Adapted affinity process to HPLC for higher purity gRNA and work in a high throughput production environment with automated fraction collection and pooling to simplify production workflow • Developed and patented two new protecting groups to improve amidite properties • Established impurity profiles for oligo synthesis that led to improved synthesis cycles and development of QC methods and product specifications• Implemented new equipment for increased throughput including oligo synthesizers, high speed concentrator, HPLCs and mass specs• Synthesized modified RNA amidites to explore the potential of TC chemistry • Helped design and evaluate a new, high-throughput DNA/RNA synthesizer and software• Provided production cost analysis for business development• Helped develop database to optimize ordering, production and tracking of RNA oligos• Developed sgRNA synthesis scale-up including reagent, equipment, and time requirements for internal and therapeutic applications (AKTA scale)

Bioscience division, specializing in oligonucleiotides synthesis and RNA interference· Completed custom synthesis projects of novel compounds for business to business activities and optimized synthetic procedures for cost reduction and scale-up· Supervised personnel and managed projects through completion· Led collaborations to develop and evaluate new RNA modifications, including all experimental design, chemical and biological analysis· As a member of the drug development team, worked with therapeutic collaborators to develop siRNA drug candidates· Synthesized degenerate and other RNA analogs to be used as new products and potential therapeutics · Performed biological analysis of new analogs including experimental design, cell culture, transfection, mRNA and protein expression, and in vitro toxicity· Evaluated alternate RNA synthesis chemistries to determine threats and opportunities to core business· Explored passive uptake of oligonucleotides using novel analogs, conjugates, enhancers, and mechanism studies to improve transfection efficiency of oligonucleotides· Synthesized and evaluated siRNA-aptamer conjugates as potential in vivo cell targeting products· Managed setting up, maintaining and running the ESI-Mass spec for analyzing small molecules and oligonucleotide QC

Startup company, acquired by ThermoFisher, specializing in RNA synthesis· Synthesized siRNA conjugates for in vitro/in vivo passive delivery· Developed multiple RNA conjugation chemistries· Synthesized, optimized, and characterized nucleic acid analogs· Contributed to the development of large scale RNA synthesis and purification processes· Supported and solved problems in chemistry, biology, production, and customer technical support · Selected as spokesperson for the company at numerous conferences and trade shows

Chemical reagents for in vitro diagnostic testing procedures· Sole person responsible for the production and inventory of over 200 reagents and biological stains · Invented and developed EPA certified formaldehyde test kits used to quickly determine the formaldehyde concentration for proper disposal· Developed and implemented QC methods as new products and equipment were introduced· Provided technical support for customers and other departments· Hired, trained, and supervised technicians

Sterile drug manufacturing facility· Responsible for the fractionation of horse plasma into antibodies used as an intravenous immuno-suppressant after transplant surgery· Supervised and developed SOP’s used to bring a new production plant into GMP compliance· Performed sterile manufacturing of drug for intravenous injection· Established production and QC parameters for controlling all buffers