Bernard Piñeiro Email & Phone Number

Lisbon, Portugal

Lisbon, Portugal

Developed and taught full stack web applications using various technologies. In my teaching role, I guide students in Java, Spring, JUnit, Hibernate, Mockito, MySQL, Maven, Ant, Tomcat, JavaScript, React, CSS, HTML, and jQuery. I ensure students grasp concepts and apply them through hands-on exercises and real-world examples.

Lisbon, Portugal

Developed and maintained websites and web apps with React, JavaScript, HTML, CSS (front-end), Scala, and Play (back-end), utilizing PostgreSQL for data storage. Worked closely with senior consultants to meet project deadlines. Played a key role in front-end design and back-end development. Attended refinement meetings and gave project updates. Also assisted with troubleshooting and debugging tasks across the stack.

Lisbon, Portugal

Responsible for offering guidance on optimal approaches, responding to client queries, supervising the creation of technical solutions, evaluating technical documentation and training CS teams.

Lisbon, Portugal

Providing assistance via phone and social media channels, I handled inquiries and address concerns from both existing customers and individuals seeking information about purchased products.

Lisbon, Portugal

Lisbon, Portugal

- Responsible for training an managing waitstaff- Responsible for managing customer concerns and complaints- Responsible for creating the most productive working environment whilst maintaining excellent level of service.

Lisbon, Portugal

Lisbon, Portugal

- Responsible for recruiting and training new staff- Assisting customers via e-mail and calls, providing information and resolving their complaints- Managing bookings via MS Project

London, England, United Kingdom

- Answering, screening and forwarding incoming e-mails and phone calls- Managing bookings via MyAllocator and MS Project.

Barcelona, Catalonia, Spain

- Answering, screening and forwarding incoming e-mails and phone calls- Managing bookings via Bananadesk- Responsible for increasing the ratings from different websites by delivering a great customer service

Rio De Janeiro, Brazil

Rio De Janeiro, Brazil

Rio De Janeiro E Região, Brasil

- Development of a protocol for mesenchymal stem cells quantification via Flow Cytometry. - Protocol optimization for mononuclear cells separation. - Growth factors and proteins quantification by ELISA: Analysis of the impact of Umbilical Cord Blood Plasma in human cell cultures according to growth factors and proteins contents. - Primary Cell cultures: Umbilical cord, Adipose tissue and Bone Marrow primary cell cultures and differentiation. - Safety and localization of Mesenchymal stromal cells derived from adipose tissue-associated hyaluronic acid. Testing the toxicity of a solution containing hyaluronic acid and adipose-derived stromal cells (ASCs) from the human adipose tissue and to assess the localization of the injected cells in rats. - Umbilical cord blood processing and storage.

Rio De Janeiro E Região, Brasil

- Optimization of NAAT diagnosis test for HIV, HBV and HCV: A nucleic acid amplification test where HIV, HBV and HCV are tested simultaneously. Working in the development of a new internal positive control by cloning a new sequence to it for probe annealing; Proven result by DNA sequencing (Sanger method); Optimization confirmed by qPCR. - HIV Primary Resistance in Brazil: Viral RNA isolation from human plasma; RT-qPCR; DNA sequencing (Sanger Method) and analysis.

Perth, Western Australia, Australia

Rio De Janeiro E Região, Brasil

- Working with HIV, HBV and HCV diagnostic kits (NAAT Kit) aiming at the improvement of viral detection in multiplex qPCR assays. - Testing new drugs targeting HIV, improving my knowledge in cell culture and virus particles handling.

Peq, Postal Code 21941-972 Rio De Janeiro - Rj

- Codon Optimization for Increased Protein Expression: Gene analysis for recombinant proteins production in different mammalian cells cultures. - Mycoplasma Detection: PCR testing and gel electrophoresis analysis for quality ensurance of all cell cultures in the laboratory. - Competent Bacteria cells preparation: bacteria cells culture; artificially competent cells method; competent bacteria cells transformation for lot analysis. - Plasmid DNA acquisition, amplification and storage: Transformation; Plasmid DNA extraction; Quantification; Restriction enzyme confirmation. - Analyses of discordant protein and mRNA expression: mRNA extraction; RT-qPCR; Protein concentration analysis by Dot-Blot and quantification by ELISA. - Mammalian cells culture: HEK-293, CHO-K1 and BHK-21 cultures for recombinant proteins production.

Computer Programming

// Programming in Java Introduction to Computer Science and Programming Version Control Systems Java Programming Language Object-Oriented.

Bachelor Of Biological Sciences, Genetics

Bachelor Of Biological Sciences, Genetics

Biotechnology Technician Degree, Biotechnology