Project: Understandng the structure of Leishmania major PPTase and its interaction with its cognate ACPArea of work: Protein purification and crystallographyProject Supervisor: Prof Suman Kundu and Dr Ravindra MakdeTechniques learned:---------------------------W e t L a b :1. Protein purification a. Hydrophobic interaction chromatography b. Ion exchange chromatography c. Nickel sepharose column/beads d. Low-pressure chromatography using BioLogic LP2. Protein structure analysis under X-Ray beamline3. Automated crystallization using Oryx8 (crystallization robot)D r y L a b / B i o i n f o r m a t i c s T o o l s :1. CCP4i suite (coot)2. Phenix3. AutoDock

Project: Purification and crystallization of putative peptidases of m24 familyArea of work: Protein purification and crystallographyProject Supervisor: Dr Ravindra MakdeTechniques learned:---------------------------W e t L a b :1. Molecular Cloning2. Protein extraction and purification a. Size exclusion chromatography b. Fast protein liquid chromatography using ÄKTApurifier 10 c. SDS-PAGE3. Protein crystallization - Microbatch, Hanging drop, Sitting dropD r y L a b / B i o i n f o r m a t i c s T o o l s :1. Serial Cloner2. PyMol3. ChimeraExtensive knowledge of NCBI features like BLAST, PubMed, etc and working with other bioinformatics tools and databases like UniProt, FASTA, ExPASy-ProtParam, XtalPred, and many other tools

Principles and working knowledge of instruments: ---------------------------------------------------------------------------1.Biochemistry and Analytical Techniques:--------------------------------------------------------------HPLC, Flame photometer, ELISA multiwell photometer, Nanodrop spectrophotometer2.Molecular Biology:--------------------------------RT-PCR, Semi-dry blotting unit, PAGE unit, UV crosslinker 3.Microscopy & Others:------------------------------------Fluoroscence microscope,Industrial autoclave,Growth chamber,Multichannel autopippettes,Digital pH penHands on experience on techniques: -----------------------------------------------------------1.Biochemical Techniques:----------------------------------------Antioxidant assay (SOD,APX,GR&CAT),Histochemical staining (for cell death and ROS viz. superoxide radicals & H2O2),Liquid chromatography (detection of hormones through HPLC)2.Molecular Biology & Genetic Engineering:---------------------------------------------------------------RNA extraction and purification, Gene elution and purification from agarose gel,Nothern blotting, qRT-PCR, Gene amplification and cloning, gradient PCR, Horizontal & Vertical gel electrophoresis, Polyacrylamide gel electrophoresis,Transformation, colony PCR3.Bioinformatics:---------------------------Primer designing4.Others:----------------Dot blot ELISA, Fluoroscence staining, Acid digestion, Plant growth and maintainence, Phenotyping, Pilot level handling of projects and experiments,Softwares: LAMINA, ImageJ---------------