Bill (William) Graham Email & Phone Number

Washington, Dc, Us

The Cannabis Tissue Culture Master Class is a three day program that takes attendees from basic TC through meristem culture, advanced storage and DNA-based technologies available to small labs. The progran is conducted in the Plant Cell Technology lab near Dupont Circle and is comprised of:Introduction to TCReview lab safety and PPEPrepare TC media togetherUnpack, and allow to coolPrepare donor plantsSet up TC workstationsPrepare cleaning solutionsCut and clean TC cuttings, some in strong Clorox and some in weak DichlorIntroduce cuttings into TC using hood and clear storage tote-Stage 1 and 2Start Seed cleaningMake sterile coir germination tubesMake liquid media for bioreactorsMake meristem culture platesDiscuss cannabis tissue culturePlant seeds in sterile coco tubesDivide established plants-Stage 3Load coupled bioreactorsDissect meristems under microscopePlace meristems on platesDiscuss factors of TC establishmentAlternate micropropagationstrategies-microsupercropping, PhytoAx, dissect flower cluster and initiate calyx pairsTroubleshooting common issuesRooting and acclimationMake and sterilize hormone-free artificial seed mediaPrepare CaCl and make artificial seedsTake tissue samples, load PCR reagentsRun LAMP-PCREvaluate PCR resultsViroid PCR workshop featuring Agdia fluorometric qPCRGenetics testing and registrationLab planning-layout and items

Rohnert Park, California, Us

Our exclusive Microclone and Tissueponics tissue culture technologies began in 2005 with our Super Starts kits, online videos and weekly workshops. Since then, Microclone has introduced tissue culture to over sixteen thousand growers and lead the industry in new and shared methods.Microclone continues to be a leader in tissue culture technology for production and crop improvement, particularly needed research of cannabis. Please contact us if we can use our CA permitted facilities to conduct your tissue culture breeding and research.

Skyfarm Vineyard is located on Fountaingrove Hill above the intersection of the Cannabis Trail and The Wine road, 101 and Mark West Springs. Our small plot of vines is dry farmed and organically pest-free. Skyfarm Vineyard is a member of the Fountaingrove Winegrowers Association and Fountaingrove AVA.

Tangent Biosciences is currently searching for 2400sf of permitted space in the SF Bay area to operate a 2500 clone/day nursery and genetic testing facility. Please message if a TC clone facility would be a valuable addition to your property.

Millersville, Maryland, Us

Seeking speakers for the Workshop on Cannabis Best Practices and Regulation presentation at the 2017 In Vitro Biology Meeting being held from June 10-14, 2017 in Raleigh, North Carolina at the Raleigh Convention Center.

The cannabis environment we have today came to be from the efforts of patients and caregivers, activists and a broad public sentiment. They have all been supported by a small, tight industry of hydro stores, their manufacturers, and the technologies they developed to make indoor garden successful and popular. Most of the legal cultivation laws we have today would not have passed if there were not domestic cannabis production. Our select group is made of hydroponic industry veterans that have distributed millions of dollars of indoor grow equipment and taught thousands of growers to use it. Our past customers are the managers and growers of the most successful cannabis operations where legally allowed. Retail grow stores and owners have been the industry contacts for growers since grow lights and soilless garden systems came out forty years ago. As grower's needs evolved, so did our knowledge and technological development. We participated in creating the tools growers use for cultivation, harvesting, processing, and marketing.The OG Group offers growroom design, staffing, management, harvesting, marketing and police interaction consulting. There is very little we have not run into in the decades each of us have been providing grow information on a daily basis.

I now have a permitted location of my own to put all of the Microclone technology to work. We are producing clones from tissue culture and raising plants in our clean environments to be guaranteed disease free. We are using pathogen and virus indexes on select strains and offering services to growers of valuable strains.

My indoor growing career started with the Marijuana Growers Guide by Ed Rosenthal. The local head shop was one of the first places I visited after I got my own car, a brand new 1982 Nightrider Trans Am because of good grades wher I had gone to buy a bong and discovered all of the great grow books for sale, including Marijuana Botany and David Gold's books. Interestingly, I would make friends with all three and many other weed book authors over the years.I first made a seedling light box in my attic, then found a bare 1000-watt halide lampa and ballast without a reflector and monkeyed something together. The next years I would set up in a friend's closed motel that was my first indoor flowering. I ordered seeds from Nevil at #TheSeedBank from an ad in #HighTimes. The original lists were printed on smelly mimeograph paper and were marked by Nevil himself. I bought Big Bud, Skunk #1, Northern Lights, and other Dutch strains. I put out one more outdoor crop in what would be my best grow location, though small, rigth across the street from my Dad's house in a 150sf clearing only 20 ft from a popular trail.In college in 1985 I set up the seed light again in my dorm room and went to work starting plants for new outdoor locations and encountered my first close call with law enforcement who responded to the fire in my dorm room caused by a lighted bottle rocket going onto a nylon duffel full of fireworks. I grew in closets and bedrooms in several houses I rented and many of those rented by friends. Setting up so many temporary grow sites from equipment not so much from hydro stores as found, stolen, and modified taught me resurcefulness and efficiency. All were 50/50 partnerships and I brought cknow-how, clones, equipment. Renters managed day-to-day, and rent and utilities. I would build over 15 partner sites and two brothers are today big growers in Humboldt. In '88 I bought a mobile home and grew for three years and again miraculously missed getting c

Established and set up independent East Coast hydroponic distribution company. Featured Phresh Filters (then called Phat), Fantech and Elicent Fans, Coliseum vertical gardens, and Agrolux Grow Lights.

Founded Micro Hydroponics from the purchased Pure Food Hydroponics started in 1995 by Bob Edberg and Jin Choi. Micro introduced hundreds of new items to the indoor garden catalogs of hydro stores across the US. I built Micro Hydroponics distribution from all of the items I created and brought in from other industries to sell on the shelves of our retail store, Pure Food Hydroponics. Pure Food had good sales before we bought it and a so-so distribution with annual sales of just over $600k. We built our catalog out of the items we made and found for our customers but that the big distributors would not carry. I picked up our Hydrofarm and Diamond Lights orders every Wed for nearly two years, 1998 and 1999, before free delivery was introduced. Stores picking up orders were allowed to walk through the facilities and offices and every few weeks I would bring in items we made, such as adding large air-cooling to hoods and lighting flip-flops, and found, such as using the router speed controllers from Harbor Freight as fan speed controllers. At that time, they only carried 1/2" blue General Hydroponics tubing. Pure Food popularized Hydroton grow rocks, Pails and lid pots, big inline fans, lightweight Phat (now Phresh) carbon filters, vertical Coliseum gardens, and innovative Agrolux grow lights from the manufacturers of Diamond Lights. I built the catalog up to over 500 items, travelled to Canada, Australia and Europe to introduce new products, and established a sales territory that reached across the US but still mostly in CA and the West, and sales that sustained $6.5 million a year. Eventually the big distributors adapted my products into their catalogs and Micro didn't add the name brands Grodan, GH, and Hortilux needed to be the first order supplier stores preferred. We did make so many of the right moves and had great relationships with our 100+ retailers. In 2003 we formed a partnership with Agrolux to form Micro Hydroponics East in Asheville, NC.

Hahn's Lighting service started as a service for maintaining commercial lighting such as changing ballasts and lamps as well as electrical services related to lighting. The owners who bought the business in the early 90's were given two Diamond Lighting systems when they bought the service company. In the years leading up to 1998, they moved into a 2600 sf warehouse and opened a hydroponic retail area in about half that space with a dedicated closed grow room. Retail hydroponics earned about $27,000/month when I joined the team at which time I dressed up and organised the retail space, upgraded the grow room to demonstrate new technology, added networked computers, a procedure handbook and expanded the retail area. When I joined the Hahn's lighting onwers to create Micro Hydroponics in 2000, sales had reached a sustained average of $154k/ month, a 570% increase. Two of the feats at Hahn's lighting I am most pleased with were, 1) Enlarging the retail space a few inches every weekend by pushing the racking back into unused space in the back to allow the addition of an entire product aisle after a year, and 2) Designing a new company sign and logo that was unexpectedly produced and delivered to the store before I had shared its plan with the owners. They were surprised and uncertain but when they learned the signman was only asking $380 for sign and logo, it was paid and installed on the side of the building that afternoon and has been the logo for trucks, shirts, cards and website ever since.

Started the first hydroponic and indoor garden supply store within two hours of Gainesville with a cell phone, Bloomington catalog, my Jeep, and my job at Lowe's Gardern Center. Customers delivered orders to me at Lowe's that I then placed by cell phone and internet (that's right, 1995 phone and internet). Customers paid me in the aisle and I gave them my car keys to retreive orders from shaded end of parking lot. The business's name "Plant Science and Garden Supply" came from an unused sign delivered to Lowes to be hung in the Garden aisle. I saved the sign and hung it over the door of my first warhouse.Plant Science was the first Sunlight Supply dealer in the state of Florida and one of it's first fifty retailers in 1996.I took a day job at Oasis Lawn Pest Control when I first opened the business and spent the day time evaluating yards for fertilizing and pest control. The door to the warehouse had a sheet of tear-off phone numbers and quartes tapes to it so customers could use the phone at the end of the building to call my cell phone. I suggesteed they visit the Hogtown Herpetological shop in from of ours or wait at the bar across from the phone.

Assisted in the lab of Alice Chase researchinging Cytoplasmic Male Sterility in maize and beans, a Mitochindrial Inheritied Trait. Skills practiced included:Sequencing DNAOperated DNA Analyzer for purification, quantification and sequencing of DNA.Daily PCRPrepared primers for DNA by column filtration method using G75 and G50 beadsExtracted DNA by Qia Quick methodAmplified DNA by RCA and BDX methods

Prepared molecular biology compounds and solutions needed for experiments by incoming graduate students, gifted middle school students and instructors. Molecular biology applications included PCR, DNA ligation, bacterial transformation, cell culture, DNA extraction, DNA restriction digest and gel electrophoresis. Plasmid DNA and mRNA preparation, SDS-PAGE and cell fractionation be differential centrifugation.

It is funny and odd to post my early grow activity but I am asked about it all the time and LInkedin is drawing most of my connections and queries, bsides I am really happy to share how it started and how much work it took to pull it off, at least half or one-third of the time. About the time I was turning 15, I was buying the third bag of weed I would ever pay for and thought, "I can grow better stuff than this, and there are seeds in to do it in this bag." I grew up weeding, planting and starting seedlings with my mom and her fabulous flower beds and we had a great potting area on the side of the house, black soil and rich homemade compost, leftover nursery pots, and every nutrient and pest control you could ask for. And we were not a homestead out of town but in a regular suberban neighborhood surrounded by a golf course. It was not hard to germinate seeds but what to do with them as they grew and were clearly identifiable. It was time to venture into the woods t the end of the street that I had was very familiar with from a decade of exploring. My playplace was becoming my grow space. The first grow spots I planted in had great sun, access and cover but were sandy and needed to be watered every day until the roots could reach the water table a few feet down. I went on a seed collecting spree and even cleaned pounds for local weed distributors t get them. Ashtrays, couch cusions, carpets, everything and everywhere I could find genetics in the area. Before the end of the summer, I had several new locations, hundreds of new little plants, and my new best tool, post-hole diggers with the handles cut to fit into a backpack. While the little plants were germinating in a (nearly) safe location at home, I would scout new sites, at first nearby and then farther and farther, where I could safely move plants and materials out of my car without being seen or challenged, and leave the car wher it would not draw suspicion. Lower leaves cut and planted foot deep

Us

Term position as Manager of Agri-Starts IV to introduce into tissue culture and micropropagate Citronella "mosquito repelling" scented geraniums. I was trained in all aspects of the laboratory, greenhouse, management, and operations by Randy Strode. Randy brought me eight 8" pots of donor plants from which I took 96 introduction cuttings. Of the 86 that survived, we multiplied and rooted them into 50,000 six inch tall rooted clones every week. We delivered over 640,000 plants from those eight mothers in less than eight months.As part of my training, I participated in the construction of the greenhouse the plants would be moved into, running wire, digging irrigation, setting up benches, constructing the carts, and installing the boiler heaters. I also drove the delivery truck at least once every week, locally and to Tampa and to post-Hurricane Andrew Homestead, including the Acosta Nurseries.

Bachelor’S Degree, Horticultural Science And Chemistry

Honors, Health Science And Biology

Education record